THE EFFECT OF KNOCKING OUT OF PVDP GENE IN THE VIRULENCE OF PSEUDOMONAS AERUGINOSA
DOI:
https://doi.org/10.22159/ijap.2024v16s5.52485Keywords:
Iron, P. aeruginosa, Pyoverdine, PvdP, Virulence factor, Biofilm, G. mellonellaAbstract
Objective: The aim of the study is to investigate the role of PvdP enzyme in the virulence of Pseudomonas aeruginosa both in vitro and in vivo.
Methods: In this study, we investigate the effect of deletion of pvdP gene on P. aeruginosa by observing its phenotypes. The observed phenotypes are the growth, pyoverdine production, motilities, 3-oxo-C12-Homo Serine Lactone (3-oxo-C12-HSL) accumulation and biofilm formation. The growth and pyoverdine production were investigated under a low iron condition while the motilities of the mutant were investigated in a semi solid media. The accumulation 3-oxo-C12-HSL was facilitated by a biosensor strain and biofilm formation was investigated using a spectrophotometer through a crystal violet staining method. The in vivo study was performed to Galleria mellonella larvae as an infection model.
Results: The deletion of the pvdP gene does not affect the growth of the P. aeruginosa but significantly reduces the production of pyoverdine. The motility properties of the bacteria were not affected by the deletion of the pvdP gene. The P. aeruginosa PvdP knock-out mutant also showed a reduction in the biofilm formation and the accumulation of 3-oxo-C12-HSL at low iron concentrations. In an in vivo experiment, the PvdP knock-out mutant caused a significantly reduced death rate of G. mellonella larvae infection model compared to the control group.
Conclusion: The findings underscore the major role of PvdP in pyoverdine production, its contribution to biofilm formation, and the motility of P. aeruginosa. Those results confirm the important role of PvdP in the virulence of P. aeruginosa in vitro and in vivo
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