FOURIER TRANSFORM INFRARED SPECTROSCOPY AND CHEMOMETRICS FOR AUTHENTICATING CHILI POWDER FROM RHODAMINE B, ERYTHROSINE B, AND PARARED
DOI:
https://doi.org/10.22159/ijap.2024.v16s6.52785Keywords:
Authentication, Chemometrics, Chili powder, FTIR spectroscopy, Synthetic dyesAbstract
Objective: The purpose of this study was to identify chili powder adulteration with synthetic dyes of rhodamine B, erythrosine B, and parared using Fourier Transform Infrared (FTIR) spectroscopy and chemometrics.
Methods: The analysis included two types of red chilies, curly red and big red chilies. FTIR and chemometric spectroscopy combination were used to detect synthetic dyes in the chili powders. Principal Component Analysis (PCA), Partial Least Square-Discriminant Analysis (PLS-DA), and Partial Least Square-Regression (PLS-R) methods were applied.
Results: The FTIR spectrum in the range of 700-850, 1100-1700, and 2300-3500 cm-1 can detect the adulteration of chili powder with rhodamine B or erythrosine B. Meanwhile, the parared dye can be distinguished at wave numbers of 2000-3000 cm-1.
Conclusion: The combination of FTIR spectroscopy with chemometrics can be used to authenticate red chili powder from rhodamine B, erythrosine B, and parared
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References
A. Ullah et al., “Banned Sudan dyes in spices available at markets in Karachi, Pakistan,” Food Addit. Contam. Part B, pp. 1–8, Aug. 2022, doi: 10.1080/19393210.2022.2100489.
I. Ripaldy, A. Wijanarka, and N. D. Putriningtyas, “Analisis Kandungan Rhodamin B pada Cabai Merah Giling di Pasar Tradisional di Kabupaten Sleman, Daerah Istimewa Yogyakarta,” Ilmu Gizi Indones., vol. 1, no. 1, pp. 10–18, 2017.
C. Li, Y. L. Wu, and J. Z. Shen, “UPLC-ESI-MS/MS analysis of sudan dyes and Para Red in food,” Food Addit. Contam. - Part A, vol. 27, no. 9, pp. 1215–1220, 2010, doi: 10.1080/19440049.2010.483600.
Djarismawati, Sugiharti, and R. Nainggolan, “Pengetahuan dan Perilaku Pedagang Cabe Merah Giling dalam Penggunaan Rhodamine B di Pasar Tradisional di DKI Jakarta,” J. Ekol. Kesehat., vol. 3, no. 1, pp. 7–12, 2005, [Online]. Available: http://ejournal.litbang.kemkes.go.id/index.php/jek/article/view/1325/1067.
N. Yuliarti, Awas bahaya di balik lezatnya makanan. Yogyakarta: Andi, 2007.
E. F. S. A. (EFSA), “Opinion of the Scientific Panel on food additives, flavourings, processing aids and materials in contact with food (AFC) to review the toxicology of a number of dyes illegally present in food in the EU,” EFSA J., vol. 3, no. 9, p. 263, Sep. 2005, doi: https://doi.org/10.2903/j.efsa.2005.263.
K. Furumiya and T. Mizutani, “Inhibition of human CYP3A4, UGT1A6, and P-glycoprotein with halogenated xanthene food dyes and prevention by superoxide dismutase.,” J. Toxicol. Environ. Health. A, vol. 71, no. 19, pp. 1307–1313, 2008, doi: 10.1080/15287390802240751.
L. Ganesan, E. Margolles-Clark, Y. Song, and P. Buchwald, “The food colorant erythrosine is a promiscuous protein-protein interaction inhibitor.,” Biochem. Pharmacol., vol. 81, no. 6, pp. 810–818, Mar. 2011, doi: 10.1016/j.bcp.2010.12.020.
A. H. Abdel Aziz, S. A. Shouman, A. S. Attia, and S. F. Saad, “A study on the reproductive toxicity of erythrosine in male mice.,” Pharmacol. Res., vol. 35, no. 5, pp. 457–462, May 1997, doi: 10.1006/phrs.1997.0158.
A. M. Ekkawy, A. Assoud, and A. M. E. L.-Z. Awahry, “Mutagenic Effects of the Food Colour Erythrosine in Rats,” 2008.
M. Gandhi and R. Mashru, “Detection of Adulterants in Red Chili Powder with Special Emphasis on Qualitative and Quantitative Estimation of Sudan I Dye In Red Chili Powder,” Int. J. Res. Rev. Vol, vol. 6, no. December, p. 12, 2019, [Online]. Available: www.ijrrjournal.com.
E. Ertaş, H. Özer, and C. Alasalvar, “A rapid HPLC method for determination of Sudan dyes and Para Red in red chilli pepper,” Food Chem., vol. 105, no. 2, pp. 756–760, 2007, doi: https://doi.org/10.1016/j.foodchem.2007.01.010.
J. K. Adjei, V. Ahormegah, A. K. Boateng, H. K. Megbenu, and S. Owusu, “Fast, easy, cheap, robust and safe method of analysis of Sudan dyes in chilli pepper powder,” Heliyon, vol. 6, no. 10, p. e05243, 2020, doi: https://doi.org/10.1016/j.heliyon.2020.e05243.
C.-F. Tsai, C.-H. Kuo, and D. Y.-C. Shih, “Determination of 20 synthetic dyes in chili powders and syrup-preserved fruits by liquid chromatography/tandem mass spectrometry,” J. Food Drug Anal., vol. 23, no. 3, pp. 453–462, 2015, doi: https://doi.org/10.1016/j.jfda.2014.09.003.
J. Li et al., “Simultaneous determination of eight illegal dyes in chili products by liquid chromatography–tandem mass spectrometry,” J. Chromatogr. B, vol. 942–943, pp. 46–52, 2013, doi: https://doi.org/10.1016/j.jchromb.2013.10.010.
L. Wang, J. Zheng, Z. Zhang, T. Wang, and B. Che, “Determination of Eight Sudan Dyes in Chili Powder by UPLC-MS/MS,” Engineering, vol. 05, no. 10, pp. 154–157, 2013, doi: 10.4236/eng.2013.510b033.
C. Li, Y. L. Wu, and J. Z. Shen, “UPLC-ESI-MS/MS analysis of Sudan dyes and Para Red in food.,” Food Addit. Contam. Part A, Chem. Anal. Control. Expo. risk Assess., vol. 27, no. 9, pp. 1215–1220, Sep. 2010, doi: 10.1080/19440049.2010.483600.
D. Chen, Y. Zhao, H. Miao, and Y. Wu, “A novel cation exchange polymer as a reversed-dispersive solid phase extraction sorbent for the rapid determination of rhodamine B residue in chili powder and chili oil,” J. Chromatogr. A, vol. 1374, pp. 268–272, 2014, doi: https://doi.org/10.1016/j.chroma.2014.11.070.
A. González-Zamora et al., “Measurement of capsaicinoids in chiltepin hot pepper: A comparison study between spectrophotometric method and high performance liquid chromatography analysis,” J. Chem., vol. 2015, 2015, doi: 10.1155/2015/709150.
H. Sun, F. Wang, and L. Ai, “Determination of banned 10 azo-dyes in hot chili products by gel permeation chromatography–liquid chromatography–electrospray ionization-tandem mass spectrometry,” J. Chromatogr. A, vol. 1164, no. 1, pp. 120–128, 2007, doi: https://doi.org/10.1016/j.chroma.2007.06.075.
M. F. Islam, M. N. Uddin, A. A. Rana, and M. M. Karim, “Development of a chemometric method for the analysis of Sudan III-IV dyes adulteration in chili powder using UV-visible spectroscopy data,” J. Sci. Innov. Res., 2018.
E. Rohaeti, K. Muzayanah, D. A. Septaningsih, and M. Rafi, “Fast analytical method for authentication of chili powder from synthetic dyes using uv-vis spectroscopy in combination with chemometrics,” Indones. J. Chem., vol. 19, no. 3, pp. 668–674, 2019, doi: 10.22146/ijc.36297.
Z.-X. Liu and J. Tan, “Qualitative and quantitative detection of Sudan I and II adulterated in chili powders by front-face synchronous fluorescence spectroscopy:Aggregation-induced emission in solid food.” Research Square, 2022, doi: 10.21203/rs.3.rs-1539582/v1.
S. A. Haughey, P. Galvin-King, Y. C. Ho, S. E. J. Bell, and C. T. Elliott, “The feasibility of using near infrared and Raman spectroscopic techniques to detect fraudulent adulteration of chili powders with Sudan dye,” Food Control, vol. 48, pp. 75–83, 2015, doi: 10.1016/j.foodcont.2014.03.047.
A. A. Bunaciu, H. Y. Aboul-Enein, and S. Fleschin, “Recent Applications of Fourier Transform Infrared Spectrophotometry in Herbal Medicine Analysis,” Appl. Spectrosc. Rev., vol. 46, no. 4, pp. 251–260, Apr. 2011, doi: 10.1080/05704928.2011.565532.
M.-Y. Dong, H.-L. Wu, W.-J. Long, T. Wang, and R.-Q. Yu, “Simultaneous and rapid screening and determination of twelve azo dyes illegally added into food products by using chemometrics-assisted HPLC-DAD strategy,” Microchem. J., vol. 171, p. 106775, 2021, doi: https://doi.org/10.1016/j.microc.2021.106775.
E. D. Purwakusumah, M. Rafi, U. D. Safitri, W. Nurcholis, and M. A. Z. Adzkiya, “Identifikasi dan Autentikasi Jahe Merah Menggunakan Kombinasi Spektroskopi FTIR dan Kemometrik (Identification and Authentication of Jahe Merah Using Combination of FTIR Spectrocopy and Chemometrics),” J. Agritech, vol. 34, no. 01, pp. 82–87, 2014, [Online]. Available: http://dx.doi.org/10.22146/agritech.9526.
A. Rahmawati, B. Kuswandi, and Y. Retnaningtyas, “Deteksi Gelatin Babi pada Sampel Permen Lunak Jelly Menggunakan Metode Fourier Transform Infra Red (FTIR) dan Kemometrik,” J. Pustaka Kesehat., vol. 3, no. 2, pp. 278–283, 2015, [Online]. Available: https://jurnal.unej.ac.id/index.php/JPK/article/view/2586.
A. Rohman, Sismindari, Y. Erwanto, and Y. B. Che Man, “Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy,” Meat Sci., vol. 88, no. 1, pp. 91–95, 2011, doi: https://doi.org/10.1016/j.meatsci.2010.12.007.
A. Rohman, Y. B. Che Man, and F. M. Yusof, “The Use of FTIR Spectroscopy and Chemometrics for Rapid Authentication of Extra Virgin Olive Oil,” J. Am. Oil Chem. Soc., vol. 91, no. 2, pp. 207–213, 2014, doi: 10.1007/s11746-013-2370-5.
Q. Ye and X. Meng, “Highly efficient authentication of edible oils by FTIR spectroscopy coupled with chemometrics,” Food Chem., vol. 385, p. 132661, 2022, doi: https://doi.org/10.1016/j.foodchem.2022.132661.
M. Surek et al., “Propolis authentication of stingless bees by mid-infrared spectroscopy and chemometric analysis,” LWT, vol. 161, p. 113370, 2022, doi: https://doi.org/10.1016/j.lwt.2022.113370.
M. Shannon et al., “The detection and determination of adulterants in turmeric using fourier-transform infrared (FTIR) spectroscopy coupled to chemometric analysis and micro-FTIR imaging,” Food Control, vol. 139, p. 109093, 2022, doi: https://doi.org/10.1016/j.foodcont.2022.109093.
H. A. Gad, S. H. El-Ahmady, M. I. Abou-Shoer, and M. M. Al-Azizi, “Application of chemometrics in authentication of herbal medicines: a review.,” Phytochem. Anal., vol. 24, no. 1, pp. 1–24, 2013, doi: 10.1002/pca.2378.
H. Zou, G. Yang, Z. Qin, W. Jiang, A. Du, and H. Y. Aboul‐Enein, “Progress in Quality Control of Herbal Medicine with IR Fingerprint Spectra,” Anal. Lett., vol. 38, no. 9, pp. 1457–1475, Jul. 2005, doi: 10.1081/AL-200062153.
I. Domínguez-Martínez, O. G. Meza-Márquez, G. Osorio-Revilla, J. Proal-Nájera, and T. Gallardo-Velázquez, “Determination of capsaicin, ascorbic acid, total phenolic compounds and antioxidant activity of Capsicum annuum L. var. serrano by mid infrared spectroscopy (Mid-FTIR) and chemometric analysis,” J. Korean Soc. Appl. Biol. Chem., vol. 57, no. 1, pp. 133–142, 2014, doi: 10.1007/s13765-013-4295-y.
D. Chen, Z. M. Wu, and H. Zhang, “Study on extraction and purification process of capsicum red pigment,” Proc. Fiber Soc. 2009 Spring Conf., vol. 1, no. 2, pp. 1450–1452, 2009, doi: 10.5539/jas.v1n2p94.
A. González-Zamora et al., “Measurement of Capsaicinoids in Chiltepin Hot Pepper: A Comparison Study between Spectrophotometric Method and High Performance Liquid Chromatography Analysis,” J. Chem., vol. 2015, p. 709150, 2015, doi: 10.1155/2015/709150.
S. Wahyuningsih, V. Anjani, H. Munawaroh, and C. Purnawan, “Optimization of Rhodamine B Decolorization by Adsorption and Photoelectrodegradation Combination System,” ALCHEMY J. Penelit. Kim., vol. 14, no. 2, p. 276, 2018, doi: 10.20961/alchemy.14.2.16440.277-290.
H. M. Zeyada, H. M. El-Mallah, T. Atwee, and D. G. El-Damhogi, “Spectroscopic studies of UV irradiated erythrosine B thin films prepared by spin coating technique,” Spectrochim. Acta Part A Mol. Biomol. Spectrosc., vol. 179, pp. 120–124, 2017, doi: https://doi.org/10.1016/j.saa.2017.02.039.
L. A. A. R. Al-Rubaie and R. J. Mhessn, “Synthesis and characterization of Azo dye para red and new derivatives,” E-Journal Chem., vol. 9, no. 1, pp. 465–470, 2012, doi: 10.1155/2012/206076.
J. N. Miller and J. C. Miller, Statistics and Chemometrics for Analytical Chemistry, 6th ed. Essex: Pearson Education Limited, 2010.
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