FOURIER TRANSFORM INFRARED SPECTROSCOPY AND CHEMOMETRICS FOR AUTHENTICATING CHILI POWDER FROM RHODAMINE B, ERYTHROSINE B, AND PARARED

STEFFY TINDA ADISTI; WIRANTI SRI RAHAYU; PRI ISWATI UTAMI; ASMIYENTI DJALIASRIN DJALIL

doi:10.22159/ijap.2024.v16s6.TY2006

Authors

STEFFY TINDA ADISTI Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto-53182, Indonesia. Rumah Sakit Umum Daerah Dr. Soeselo, Jl. Dr. Sutomo No. 63, Slawi, Jawa Tengah-52419, Indonesia
WIRANTI SRI RAHAYU Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto-53182, Indonesia
PRI ISWATI UTAMI Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto-53182, Indonesia
ASMIYENTI DJALIASRIN DJALIL Department of Pharmacy, Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Purwokerto-53182, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s6.TY2006

Keywords:

Authentication, Chemometrics, Chili powder, FTIR spectroscopy, Synthetic dyes

Abstract

Objective: The purpose of this study was to identify chili powder adulteration with synthetic dyes of rhodamine B, erythrosine B, and parared using Fourier Transform Infrared (FTIR) spectroscopy and chemometrics.

Methods: The analysis included two types of red chilies, curly red and big red chilies. FTIR and chemometric spectroscopy combination were used to detect synthetic dyes in the chili powders. Principal Component Analysis (PCA), Partial Least Square-Discriminant Analysis (PLS-DA), and Partial Least Square-Regression (PLS-R) methods were applied.

Results: The FTIR spectrum in the range of 700-850, 1100-1700, and 2300-3500 cm^-1 can detect the adulteration of chili powder with rhodamine B or erythrosine B. Meanwhile, the parared dye can be distinguished at wave numbers of 2000-3000 cm^-1.

Conclusion: The combination of FTIR spectroscopy with chemometrics can be used to authenticate red chili powder from rhodamine B, erythrosine B, and parared.

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