IN VITRO ANTIBACTERIAL ACTIVITY ROSEMARY OIL AGAINST ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN SYRIA

Authors

  • LUBNA RABIE Department of Biochemistry and Microbiology, Faculty of Pharmacy, Ebla Private University, Aleppo, Syria.
  • NOURA BERAKDAR Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy, Ebla Private University, Aleppo, Syria.

DOI:

https://doi.org/10.22159/ijls.2022.v10i1.46501

Keywords:

Escherichia coli, Rosemary oil, Well diffusion testing

Abstract

Objective: The aim of this research is to determine the antibacterial activity of rosemary oil against Escherichia coli that causes the urinary tract infection) UTI).

Methods: An in vitro study was carried out using the following bacterial strains involved in UTI diseases using well diffusion (WD) testing: E. coli (ATCC 25922) and 25 strains were compiled from Aleppo Hospital. It was from people and has UTI. The antibacterial activity of rosemary oil was determined in the form of inhibition zone using agar WD testing.

Results: The obtained results indicated that rosemary oil has an inhibitory effect on E. coli (ATCC 25922) and 10 strains. This study showed that rosemary oil has antibacterial activity.

Conclusion: Rosemary oil has shown an antibacterial effect that causes UTIs, so we suggest using this oil in the treatment of UTIs.

References

Zagaglia C, Ammendolia MG, Maurizi L, Nicoletti M, Longhi C. Urinary tract infections caused by uropathogenic Escherichia coli strains-new strategies for an old pathogen. Microorganisms 2022;10:1425.

Hooton TM, Bradley SF, Cardenas DD, Colgan R, Geerlings SE, Rice JC, et al. Diagnosis, prevention, and treatment of catheterassociated urinary tract infection in adults 2009: International clinical practice guidelines from the infectious diseases society of America, urinary catheter guidelines. Clin Infect Dis 2010;50:625-63.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.

Olivas-Méndez P, Chávez-Martínez A, Santellano-Estrada E, Asorey LG, Sánchez-Vega R, Rentería-Monterrubio AL, et al, Antioxidant and antimicrobial activity of rosemary (Rosmarinus officinalis) and garlic (Allium sativum) essential oils and chipotle pepper oleoresin (Capsicum annum) on beef hamburgers. Foods 2022;11:2018.

Bradshaw LJ. Laboratory Microbiology. 4th ed. Fort Worth: Saunders College Publishing; 1992.

Alves TM, Silva AF, Brandão M, Grandi TS, Smânia E, Júnior AS. Biological screening of Brazilian medicinal plants. Mem Inst Oswaldo Cruz 2000;95:367-73.

Geetha RV, Roy A, Lakshmi T. Nature’s weapon against urinary tract infectons. IJDDR 2011;3:85-100.

Verma RS, Padalia RC, Chauhan A, Upadhyay RK, Singh VR. Productivity and essential oil composition of rosemary (Rosmarinus officinalis L.) harvested at different growth stages under the subtropical region of North India. J Essent Oil Res 2020;32:1-6.

Shokova EA, Kim J, Kovalev. Camphor and its derivatives. Unusual transformations and biological activity. Russian J Organ Chem 2016;52:459-88.

Aparicio S, Alcalde R, Dávila MJ, García B, Leal JA. Properties of 1,8-Cineole: A thermophysical and theoretical study. J Phys Chem B 2007;111:3167-77.

Neeman EM, Moreno JR, Huet TR. The gas phase structure of α-pinene, a main biogenic volatile organic compound. J Chem Phys 2017;147:214305.

Wang L, Zhang K, Zhang K, Zhang J, Fu J, Li J, et al. Antibacterial activity of Cinnamomum camphora essential oil on Escherichia coli during planktonic growth and biofilm formation. Front Microbiol 2020;11:561002.

Wu K, Lin Y, Chai X, Duan X, Zhao X, Chun C. Mechanisms of vapor-phase antibacterial action of essential oil from Cinnamomum camphora var. linaloofera Fujita against Escherichia coli. Food Sci Nutr 2019;7:2546-55.

Published

12-11-2022

How to Cite

RABIE, L., & BERAKDAR, N. (2022). IN VITRO ANTIBACTERIAL ACTIVITY ROSEMARY OIL AGAINST ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN SYRIA. Innovare Journal of Life Sciences, 10(1), 1–4. https://doi.org/10.22159/ijls.2022.v10i1.46501

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Original Article(s)