BIOINFORMATIC CHARACTERIZATION OF SOD UNDER UV IN FENUGREEK (FAMILY: FABACEACE)
Abstract
Objective: To perform In silico analysis of superoxide dismutase (SOD) of the Fabaceae family along with the assessment of its physiological significance under UV irradiation in fenugreek belongs to the same family.
Methods: In silico study was carried out for the superoxide dismutase of Fabaceae and isozyme expression in the fenugreek was measured subjected to UV exposure during the post-germination phase.
Results: The expression of the enzyme was found constitutive in both the treated and control sets. On gel analysis exhibited a significant enhancement in the expression of enzymes under UV exposure especially at 365 nm. Computational analysis of SOD of Fabaceae revealed that the physicochemical properties and secondary structural features of the isoforms differ to some extent. It was also found that the motif sharing pattern and the conserved domain were different for each isoform, which may be attributed to the difference in cofactor involved.
Conclusion: The results suggest that the elevation of SOD activity might be responsible towards overcoming the stress imposed by UV irradiation. Thus, the role of SOD may be beneficial for oxidative stress management in plants during germination phases.
Keywords: In-silico Analysis, Superoxide dismutase, Trigonella foenum-graecum, UV irradiation
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References
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