• Saran Kumar Gupta Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, West Bengal, India
  • Najnin Islam Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, West Bengal, India
  • Chandrani Choudhuri Department of Botany, North Bengal St. Xavier's College, Rajganj 735134, Jalpaiguri, West Bengal, India
  • Palash Mandal Plant Physiology and Pharmacognosy Research Laboratory, Department of Botany, University of North Bengal, Siliguri 734013, West Bengal, India




Trigonella foenum-graecum, Antidiabetic activity, Antioxidant enzymes, Oxidative stress, Phenolic compounds, UV irradiation


Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase.

Methods: The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed.

Results: Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement in free radical scavenging as well as antidiabetic activity at the 48h stage of germination. Interestingly highest phenol and flavonol content were also stimulated at the same stage by UV 365 nm. The effect of UV irradiation was evident on the localisation of H2O2 and superoxide radical. The on gel assay revealed that the total activities of the antioxidant enzymes were extensively elevated by UV irradiation.

Conclusion: The results suggest that the UV irradiation technology can be implemented for the enhancement of nutraceutical properties of sprouts also UV exposure helps in the induction of the antioxidant enzyme system which may be beneficial for oxidative stress management in plants during germination phases.


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How to Cite

Gupta, S. K., N. Islam, C. Choudhuri, and P. Mandal. “ELICITATION OF THERAPEUTIC POTENTIAL AND OXIDATIVE STRESS ASSESSMENT OF FENUGREEK SPROUTS UNDER UV IRRADIATION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 9, no. 5, May 2017, pp. 91-99, doi:10.22159/ijpps.2017v9i5.15647.



Original Article(s)