• Said-al Ahl Hah Medicinal and Aromatic Plants Researches Department, National Research Centre, 33 El-Bohouth St., (former El- Tahrir St.,) Dokki, Giza, Egypt. Postal Code: 12622
  • Sabra As
  • Alzuaibr Fma
  • Ramadan Mf
  • Gendy Ash


Objective: The objective of this research was to study the effects of water stress treatments (40, 60, and 80 % available soil moisture, ASM) and/or potassium application (0, 0.4 and 0.8 g/pot) on growth parameters, yield and fatty acids content and composition of Oenothera biennis under greenhouse conditions.

Methods: A two years pot experiment was conducted on Oenothera biennis under Egypt conditions in 2013/2014 and 2014/2015 seasons. Growth, seed yield (g/plant) and seed fixed oil content recorded at the first and second seasons. The fatty acid profile of total lipids extracted from Oenothera biennis was determined by Gas-liquid chromatography (GLC) analysis.

Results: Growth characteristics (plant height, the number of branches, the number of capsules/plant and dry weights of the whole plant, root, and straw), seed yield (g/plant) and oil yield in two seasons were significantly decreased with the rise in water stress levels. Oil % was stimulated in response to water stress. Application of potassium counteracted the adverse effects of water stress. The maximum growth, seed yield and oil yield were obtained from plants irrigated with 80 % available soil moisture (ASM) plus potassium (0.8 g/pot). On the contrary, supplying plants with a water level of 40 % ASM and with potassium (0.8 g/pot) or (0.4 g/pot) gave the best result for the oil percentage in the first and second seasons, respectively. In respect to fatty acids profile, the percentage of C16:0, C18:1n9 and C22:0 acids were increased with increasing water stress while a reverse response was observed in C18:0, C18:2n6, C20:0, C18:3n6 and C20:1n9 acids. K application attenuated oil composition, where it led to a slight increase in C18:2n6 and C20:0 acids while decreased the percentages of C16:0, C18:1n9, C22:0 acids C18:0, C18:3n6 and C20:1n9 acids. Potassium rates plus 60 % ASM increased C18:0 and C18:1n9 acids while K application with both of 60 % ASM and 40 % ASM increased C18:2n6 and C20:0 acids. The C22:0 acids increased under the interaction of all irrigation treatments with (0.4 g/pot) dose of K. However, C16:0 acids increased as a result of 80 % ASM treatment with the different potassium rates. This study demonstrated the beneficial effects of K application to alleviating the adverse effects of water stress on Oenothera plants.

Conclusion: Increasing irrigation levels increased the plant height, the number of branches, the number of capsules/plant, seed yield and dry weights of the whole plant, root, and straw of Oenothera biennis and the optimum irrigation level for the highest yields of these variables was 80 % ASM. Whereas, oil % decreased with increasing irrigation levels and the optimum irrigation level for the highest oil % was 40 % ASM. However, for the oil yield from plants that received 80 % ASM produced more oil yield than plants received 60 % or 40 % ASM. Application of potassium counteracted the adverse effects of water stress. Potassium fertilizer increased plant height, the number of branches, the number of capsules/plant, seed yield and dry weights of the whole plant, root, and straw of Oenothera biennis. Application of potassium could be a practical approach for enhancing the oil accumulation in Oenothera biennis. The current study provided important information about the qualitative and quantitative changes in the fatty acids profile of Oenothera biennis in relation to potassium application under water stress conditions.

Keywords: Fatty acids, Oenothera biennis, Potassium fertilizer, Water stress


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Sarker BC, Hara M, Uemura M. Proline synthesis, physiological responses and biomass, the yield of eggplants during and after repetitive soil moisture stress. Sci Hortic 2005;103:387-402.

Bouchereau A, Clossais-Besnard N, Bensaoud A, Leport L, Renard M. Water stress effects on rapeseed quality. Eur J Agron 1996;5:19-30.

Esmaeilian Y, Sirousmehr AR, Asghripour MR, Amiri E. Comparison of the sole and combined nutrient application on yield and biochemical composition of sunflower under water stress. Int J Appl Sci Technol 2012;2:2014-220.

Marschner H. Mineral nutrition of higher plants. 2nd Ed. Academic Press: San Diego, California, USA; 1995.

Ford CW, Wilson JR. Changes in levels of solutes during osmotic adjustment to water stress in leaves of four tropical pasture species. Aust J Plant Physiol 1981;8:77-91.

Andersen MN, Jensen CR, Losch R. The interaction effects of potassium and drought in field-grown barley. I. yield, water-use efficiency, and growth. Acta Agric Scandinavica Section B-Soil Plant Sci 1992;42:34-44.

Sharma KD, Nandwal AS, Kuhad MS. Potassium effects on CO2 exchange, ARA and yield of cluster bean cultivars under water stress. J Potassium Res 1996;12:412-23.

Tiwari HS, Agarwal RM, Bhatt RK. Photosynthesis, stomatal resistance and related characters as influenced by potassium under normal water supply and water stress conditions in rice (Oryza sativa L.). Indian J Plant Physiol 1998;3:314-6.

Egilla JN, Davies FT, Drew MC. Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. leprechaun: plant growth, leaf macro and micronutrient content and root longevity. Plant Soil 2001;229:213-24.

Premachandra G, Saneoka H, Ogata S. Cell membrane stability and leaf water relations as affected by potassium nutrition of water–stressed maize. J Exp Bot 1991;42:739-45.

Nandwal AS, Hooda A, Datta D. Effect of substrate moisture and potassium on water relations and C, N and K distribution in Vigna radiata. Biologia Planatarium 1998;41:149-53.

Carter JP, Gamma-linolenic acid as a nutrient. Food Technol 1988;4:72-82.

Johnson MM, Swan DD, Surette ME, Stegner J, Chilton T, Fonteh AN, et al. Dietary supplementation with γ-linolenic acid alters fatty acid content and eicosanoid production in healthy humans. J Nutr 1997;127:1435-44.

Murphy CL, McKenny CB, Auld DL, Hopper NW. Field production of texas native evening primrose (Oenothera spp.) as a source of gamma-linolenic acid. Acta Horticulturae 2004;629:283-8.

Horrobin DF. Nutritional and medicinal importance of γ-linolenic acid. Prog Lipid Res 1992;31:163-94.

Deng Y, Hua H, Li J, Lapinskas P. Study on evening primrose (Oenothera biennis) in China. Econ Bot 2001;55:83-92.

Levy A, Palevitch D, Raneen C. Increasing gamma linolenic acid in evening primrose grown under hot temperatures by breeding early cultivars. Acta Hortic 1993;330:219-25.

Honermeier B, Ghasemnezhad A, Beiten S. Effect of different autumn and spring sowing times on seed yield and seed quality of evening primrose (Oenothera biennis L.). J Med Spice Plants 2005;10:187-93.

Sikha S, Sunil P, Arti J, Sujata B, Navdeep D, Kranti T. Effect of salt stress on seedling growth and survival of Oenothera biennis L. Int Res J Environ Sci 2014;3:70-4.

Jackson ML. "Soil Chemical Analysis" Published by prentice Hall of Indian Private Limited. M.97, Connght Citrus, New Delhi-1; 1973.

Black CA. Methods of soil analysis. Part. I. American Society of Agronomy; 1965.

AOCS, Official methods and recommended practices of the American Oil Chemists Society, 4th ed, edited by AOCS. Champaign, IL Official Method Ai 2 75, reapproved; 1993.

ISO, International Standard 5509, Animal and vegetable fats and oils-preparation of methyl esters of fatty acids. ISO, Geneva; 2000.

Rezaei A, Yazdanpanah M, Khosrotaj Z, Bahreman Z, Poureidi S, Rashnavadi R, et al. Effect of drought stress on yield and yield components of pea hybrids. Tech J Eng Appl Sci 2014;4:324-7.

Shirani Rad AH, Zandi P. The effect of drought stress on qualitative and quantitative traits of spring rapeseed (Brassica napus L.) cultivars. Agriculture 2012;99:47-54.

Forcella F, Gesch RW, Isbell TA. Seed yield oil, and fatty acids of cuphea in the northwestern corn belt. Crop Sci 2005;45:2195-202.

Gunasekara CP, Martin LD, French RJ, Siddique KHM, Walton G. Genotype by environment interactions of Indian mustard (Brassica juncea L.) and canola (Brassica napus L.) in Mediterranean-type environments: I. crop growth and seed yield. Eur J Agron 2006;25:1-12.

Pascale SD, Paradiso R, Barbieri G. Recovery of physiological parameters in Gladiolus under water stress. Colture Protette 2001;30:65-9.

Silber A, Xu G, Wallach R. High irrigation frequency: the effect on plant growth and on the uptake of water and nutrients. Acta Horticulturae 2003;627:89-96.

Ashraf M, O'Leary JW. Effect of drought stress on growth, water relations and gas exchange of two lines of sunflower differing in degree of salt tolerance. Int J Plant Sci 1996;157:729-32.

Bradford KJ, Hsiao TC. Physiological responses to moderate water stress. In: Encyclopedia of plant physiology, new series, vol. 12B, Physiological plant ecology II, Water relations, and carbon assimilation. ed. OL Lange, PS Nobel, CB Osmond, H Ziegler. Springer-Verlag, Berlin; 1982.

Rambal S, Debussche G. Water balance of Mediterranean ecosystems under a changing climate. Global change and Mediterranean-type ecosystems. Ecological studies. Vol. 117. ed. by Jose M Moreno, Walter C Oechel. Springer-Verlag, New York; 1995.

Singh N, Chhokar V, Sharma KD, Kuhad MS. Effect of potassium on water relations, CO2 exchange and plant growth under quantified water stress in chickpea. Indian J Plant Physiol 1997;2:202-6.

Hussain F, Malik AU, Haji MA, Malghani AL. Growth and yield response of two cultivars of mungbean (Vigna radiata L.) to different potassium levels. J Animal Plant Sci 2011;21:622-5.

Lu L, He C, Jin Y, Xing l, Zhang Z, Wei J. Effects of the applications of phosphorus and potassium fertilizers at different growth stages on the root growth and bioactive compounds of Salvia miltiorrhiza Bunge. Aust J Crop Sci 2013;7:1533-43.

Das PC. Plant nutrients. In: Manures and Fertilizers. 2nd Edition. Kalyani Publishers: New Dehli, India; 1999.

Sahai VN. Mineral Nutrients. In: Fundamentals of Soil. 3rd Edition. Kalyani Publishers, New Dehli, India; 2004.

Asgar A, Nadeem MA, Tahir ATM, Hussain M. Effect of different potash levels on the growth, yield and protein contents of chickpea. Pakistan J Bot 2007;39:523-7.

Rahimi MM, Salahizadeh AA. Effect of different levels of irrigation and potassium on qualitative and quantitative characteristics of the beans in Yasooj, Iran. Eur Online J Nat Soc Sci 2015;4:50-6.

Sangakkara UR, Frehner M, Nosberger J. Influence of soil moisture and fertilizer potassium on the vegetative growth of mungbean (Vigna radiata L.) and cowpea (Vigna unguiculata L.). J Agron Crop Sci 2001;186:73-81.

Fanaeia HR, Galavia M, Kafib M, Bonjar AG. Amelioration of water stresses by potassium fertilizer in two oilseed species. Int J Plant Prod 2009;3:41-54.

Khan AH, Mujtaba SM, Khanzada B. Responses of growth, water relation and solute accumulation in wheat genotypes under water deficit. Pakistan J Bot 1999;31:461-8.

Maddonni GA, Otegui ME, Cirilo AG. Water stress and potassium effects plant population row spacing and canopy architecture in hybrid maize. Field Crops Res 2001;71:183-93.

Chow WS, Ball MC, Anderson JS. Growth and photosynthetic responses of spanish to salinity implication of K nutrition for salt tolerance. Aust J Plant Physiol 1990;17:563-78.

Reiner H, Ceylan A, Marquard R. Agronomix performance of evining primrose (Oenothera bieniss L.) in turkey and Germany. Eucarpia Congress Gottingen 1989;20:5.

Fieldsend AF, Morison JIL. Climatic conditions during seed growth significantly influence oil content and quality in winter and spring evening primrose crops (Oenothera spp.). Ind Crops Prod 2000;12:137-47.

Hudson BJF. Evening primrose (Oenothera spp.) oil and seed. J Am Oil Chem Soc 1984;61:540-3.

Eslami M, Dehghanzadeh H, Najaf F. The effect of drought stress on oil percent and yield and the type of sunflower (Helianthus annuus L.) fatty acids. Agric Sci Dev 2015;4:4-6.

Razi H, Asad MT. Evaluation of variation of agronomic traits and water stress tolerant in sunflower conditions. Agric Nat Resour Sci 1998;2:31-43.

Al-Palsan M, Boydak E, Hayta M, Gercek S, Simsek M. Effect of row space and irrigation on seed composition of Turkish sesame. J Am Oil Chem Soc 2001;78:933-5.

Afshar RK, Chaichi MR, Rezaei K, Asareh MH, Karimi M, Hashemi M. Irrigation regime and organic fertilizers influence on oil content and fatty acid composition of milk thistle seeds. Agron J 2015;107:187-94.

Navari-Izzo F, Vangioni N, Quartacci MF. Lipids of soybean and sunflower seedlings grown under drought conditions. Phytochemistry 1990;29:2119-23.

Chaiyadeea S, Jogloya S, Songsria P, Singkhama N, Vorasoota N, Sawatsitang P, et al. Soil moisture affects fatty acids and oil quality parameters in peanut. Int J Plant Prod 2013;7:81-96.

Wilson RF, Burke JJ, Quisenberry JE. Plant morphological and biochemical responses to field water deficits. II. Responses of leaf glycerolipid composition in cotton. Plant Physiol 1987;84:251-4.

Pham Thi AT, Borrel-Flood C, Vieira SJ, Justin AM, Mazliak P. Effects of drought on [1-14C]-oleic acid and [1-14C]-linoleic acid desaturation in cotton leaves. Physiol Plant 1987;69:147-50.

Pham Thi AT, Borrel-Flood C, Vieira SJ, Justin AM, Mazliak P. Effects of water stress on lipid metabolism in cotton leaves. Phytochemistry 1985;24:723-7.

Ozkan A, Kulak M. Effects of water stress on growth, oil yield, fatty acid composition and mineral content of Sesamum indicum. J Anim Plant Sci 2013;23:1686-90.

Ahmad R, Saeed M, Ullah E, Mahmood T. Effect of potassium on protein, oil and fatty acid contents in two autumns planted sunflower hybrids. Int J Agric Biol 1999;1:325-7.

Sawana ZM, Hafezb SA, Basyonyb AE, Alkassas AR. Nitrogen, potassium, and plant growth retardant effects on oil content and quality of cotton seed. Grasas Aceites 2007;58:243-51.

Dos Santos JI, da Silvaa TRB, Rogérioa F, Santos RF, Secco D. Yield response in crambe to potassium fertilizer. Ind Crops Prod 2013;43:297-300.

Yin X, Vyn TJ. Potassium placement effects on yield and seed composition of no-till soybean seeded in alternate row widths. Agron J 2003;95:126-32.

Montenegro OR, Magnitskiy S, Henao MCT. Effect of nitrogen and potassium fertilization on the production and quality of oil in Jatropha curcas L. under the dry and warm climate conditions of Colombia. Agronomía Colombiana 2014;32:255-65.

Berti MT, Fischer SU, Wilckens RL, Hevia MF, Johnson BL. Borage (Borago officinalis L.) response to N, P, K, and S fertilization in south-central Chile. Chilean J Agric Res 2010;70:228-36.

Severino LS, Ferreira GB, Moraes CRA, Gondim TMS, Freire WSA, Castro DA, et al, Growth and yield of castor bean fertilized with macronutrients and micronutrients. Pesqui Agropecu Bras 2006;41:563–8.

Khan HZ, Malik MA, Saleem MF, Aziz I. Effect of different potassium fertilization levels on growth, seed yield and oil contents of canola (Brassica napus L.). Int J Agric Biol 2004;6:557-9.

Pande M, Goli MB, Bellaloui N. Effect of foliar and soil application of potassium fertilizer on soybean seed protein, oil, fatty acids, and minerals. Am J Plant Sci 2014;5:541-8.

Bellaloui N, Yin X, Mengistu A, McClure AM, Tyler DD, Reddy KN. Soybean seed protein, oil, fatty acids, and isoflavones altered by potassium fertilizer rates in the Midsouth. Am J Plant Sci 2013;4:976-88.

Mahmood T, Saeed M, Ahmad R. Impact of water and potassium management on yield and quality of maize (Zea mays L.). Pakistan J Biol Sci 2000;3:531-3.



How to Cite

Hah, S.- al A., S. As, A. Fma, R. Mf, and G. Ash. “GROWTH, YIELD AND FATTY ACIDS RESPONSE OF OENOTHERA BIENNIS TO WATER STRESS AND POTASSIUM FERTILIZER APPLICATION”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 4, Apr. 2016, pp. 77-82,



Original Article(s)