GC-MS ANALYSIS AND EVALUATION OF BIOACTIVITIES OF KAEMPFERIA PARISHII - A NATURAL SOURCE OF TOTAROL
Keywords:
Kaempferia parishii, GC-MS analysis, Totarol, Phytol, Antioxidant activity, Antimicrobial activityAbstract
Objective: The present study was aimed for phytoconstituent analysis, in vitro antioxidant and antimicrobial activities of leaf and rhizome extracts of an unexplored plant, Kaempferia parishii (Zingiberaceae).
Methods: The extracts were analyzed by gas chromatography/mass spectrometry to determine volatile chemical constituents. Antioxidant activity of extracts was determined using DPPH assay whereas the antimicrobial effects were tested by inhibition zone diameter and minimum inhibitory concentration.
Results: GC/MS analysis revealed the presence of 7 and 8 identified components accounting for 92.1% and 82.86% of the leaf and rhizome extract of Kaempferia parishii respectively. In leaf extract phytol (72.55±0.5%), hexadecanoic acid methyl ester (4.94±0.2%), hexahydro farnesyl acetone (3.78±0.2%), dibutyl phthalate (3.31±0.2%) were found to be the major constituents and those of rhizome extract were totarol (74.96±0.86%), cembrene (2.83±0.2%), borneol (1.23±0.15). Both the extracts exhibited low to moderate antioxidant activity. They possess very weak activities against some tested microorganisms while the extracts had no activity against some microorganisms.
Conclusion: Totarol, an antimicrobial agent, was found to be the major constituent of Kaempferia parishii rhizome extract. Thus, Kaempferia parishii can be used as a natural source of totarol. This is the first report on the unexplored plant, Kaempferia parishii.
Keywords: Kaempferia parishii, GC-MS analysis, Totarol, Phytol, Antioxidant activity, Antimicrobial activity
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