MOLECULAR CHARACTERIZATION OF ENDANGERED MEDICINAL PLANT SPECIES HEDYCHIUM CORONARIUM FROM EASTERN INDIA
Keywords:Hedychium coronarium, ISSR, RAPD, Molecular characterization
Objective: Molecular characterization of Hedychium coronarium from 4 different populations of Odisha using 9 inter simple sequence repeats and 15 random amplified polymorphic DNA markers to indicate the closeness of species and hybrids quickly and efficiently.
Methods: A dendrogram was constructed through sequential agglomerative hierarchial and nested (SAHN) clustering and un-weighted pair group method with arithmetic mean (UPGMA) analysis using Jaccard's similarity coefficient of combined markers using this particular species.
Results: Two major clusters were found, i.e., cluster-I (Malkangiri-1, Phulabani-1, Phulabani-3, Malkangiri-2, Khurda-1, Khurda-2, Khurda-3, Angul-3, Angul-1, Angul-2 and Phulabani-2) and cluster-II (Malkangiri-3). The clustering pattern also revealed moreover the extent of genetic similarity between germplasms collected from four different regions population.
Conclusion: The potential of this technique would be further realised to the fullest extent for the identification and tagging of the important novel gene in different taxa, unexplored yet, thus facilitating the improvement of desired taxa of Zingiberaceae. The findings would be of immense enough significance for complementing the strategies of conservation and characterization of these important taxa of Zingiberaceae following modern biotechnological approach.
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