UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND ACECLOFENAC IN BULK AND TABLET DOSAGE FORM
Keywords:
UV spectrophotometric method, Tramadol Hydrochloride, Aceclofenac, Simultaneous estimation, Tablet dosage form, ValidationAbstract
Objective: To develop a simple, economic and validated UV spectrophotometric method for the simultaneous estimation of aceclofenac and tramadol hydrochloride in bulk and tablet dosage form.
Methods: The UV spectrophotometric method for simultaneous estimation of aceclofenac and tramadol hydrochloride has been developed. Methanol and water in the ratio 60:40 was used as the solvent. Tramadol hydrochloride and aceclofenac showed maximum absorbance at wavelength 214.8 nm and 275.6 nm respectively.
Results: The developed method showed tramadol hydrochloride and aceclofenac to be linear in the concentration range of 5–30 µg/ml with a correlation coefficient of 0.998 and 0.999 respectively. The result of recovery studies for the tablet was found to be in the range of 98.125%-101.0% and 98.01%-98.36% for tramadol hydrochloride and aceclofenac respectively.
Conclusion: The results show that the developed UV spectrophotometric method is simple, economical, accurate, precise and repeatable.
Keywords: UV spectrophotometric method, Tramadol Hydrochloride, Aceclofenac, Simultaneous estimation, Tablet dosage form, Validation
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References
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