QUANTITATIVE ANALYSIS OF Î‘-MANGOSTIN IN MANGOSTEEN (GARCINIA MANGOSTANA L.) PERICARP EXTRACT FROM FOUR DISTRICT OF WEST JAVA BY HPLC METHOD
Keywords:Xanthone, Nil, HPLC, Acidic methanol, Validation method
Objective: The objective of this study was to determine the validity of analytical methods of Î±-mangostin using HPLC with UV detector and acidic methanol as mobile phase. Then, compare the level of Î±-mangostin and quality of mangosteen pericarp from four regions in West Java.
Methods: The determination was performed by HPLC with C-18 (octadecylsilane) as a column with methanol and 1% acetic acid in water (95:5) as mobile phase and a flow rate of 1 ml/min. UV detector was adjusted at 246 nm
Results: The calibration curves for the Î±-mangostin were linear over concentrations range from 5 to 200 Âµg/ml with a correlation coefficient (r) 0.9999. The coefï¬cient of variation obtained from Î±-mangostin was less than 2 %. LOD and LOQ of Î±-mangostin were 2.122 and 7.072 Î¼g/ml, respectively. The levels of Î±-mangostin from four regions were 12.39 % for Subang, 8.30 % for Purwakarta, 6.34 % for Bogor and 5.70 % for Tasikmalaya.
Conclusion: The modified HPLC method meets the validation criteria. The highest a-mangostin level and the best pericarp quality was mangosteen from Subang.
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