• Muchtaridi Muchtaridi Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl KM 21.5 Bandung-Sumedang, Jatinangor.
  • Devi Suryani
  • Warid Ali Qosim
  • Nyi Mekar Saptarini


Xanthone, Nil, HPLC, Acidic methanol, Validation method


Objective: The objective of this study was to determine the validity of analytical methods of α-mangostin using HPLC with UV detector and acidic methanol as mobile phase. Then, compare the level of α-mangostin and quality of mangosteen pericarp from four regions in West Java.

Methods: The determination was performed by HPLC with C-18 (octadecylsilane) as a column with methanol and 1% acetic acid in water (95:5) as mobile phase and a flow rate of 1 ml/min. UV detector was adjusted at 246 nm

Results: The calibration curves for the α-mangostin were linear over concentrations range from 5 to 200 µg/ml with a correlation coefficient (r) 0.9999. The coefï¬cient of variation obtained from α-mangostin was less than 2 %. LOD and LOQ of α-mangostin were 2.122 and 7.072 μg/ml, respectively. The levels of α-mangostin from four regions were 12.39 % for Subang, 8.30 % for Purwakarta, 6.34 % for Bogor and 5.70 % for Tasikmalaya.

Conclusion: The modified HPLC method meets the validation criteria. The highest a-mangostin level and the best pericarp quality was mangosteen from Subang.


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How to Cite

Muchtaridi, M., D. Suryani, W. A. Qosim, and N. M. Saptarini. “QUANTITATIVE ANALYSIS OF Α-MANGOSTIN IN MANGOSTEEN (GARCINIA MANGOSTANA L.) PERICARP EXTRACT FROM FOUR DISTRICT OF WEST JAVA BY HPLC METHOD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 8, Aug. 2016, pp. 232-6, https://journals.innovareacademics.in/index.php/ijpps/article/view/12297.



Original Article(s)