OPTIMIZATION OF BACOSIDE A LOADED SNEDDS USING D-OPTIMAL MIXTURE DESIGN FOR ENHANCEMENT INSOLUBILITY AND BIOAVAILABILITY

Mukesh Gohel; Ankita Purohit; Asha Patel; Lal Hingorani

doi:10.22159/ijpps.2016v8i12.13488

Authors

Mukesh Gohel Anand Pharmacy College, Anand, Gujarat, India
Ankita Purohit Anand Pharmacy College, Anand, Gujarat, India
Asha Patel Parul Institute of Pharmacy and Research, Vadodara, Gujarat, India
Lal Hingorani Pharmanza Herbal Pvt. Ltd, Dharmaj, Gujarat, India

DOI:

https://doi.org/10.22159/ijpps.2016v8i12.13488

Keywords:

Bacoside A, Solubility, D-optimal mixture design, Bioavailability

Abstract

Objective: The objective of present study is to enhance solubility and bioavailability of poorly soluble bacoside A present in Bacopa monnieri extract using self nano emulsifying drug delivery system (SNEDDS) for the treatment of Alzheimer's disease.

Methods: Solubility of the drug was assessed in various oils (edible as well as synthetic oil), surfactant and co-surfactant by saturation solubility study. Pseudo-ternary phase diagram was used to obtain appropriate concentration ranges of components include oil, surfactant and co-surfactant.

Results: From the result of saturated solubility study and phase diagram, oleic acid, tween 20 and ethanol was selected as oil, surfactant and co-surfactant. The D-Optimal mixture design was used to optimize the formulation on the basis of solubility of drug and dilution potential. In vitro dissolution, study showed 89% of drug release from optimized SNEDDS formulation compared to untreated drug extract with 24% of drug release in 60 min. Ex vivo diffusion study showed more than 90% of drug diffused from optimized SNEDDS formulation compared to pure extract.

Conclusion: In a nutshell, the developed SNEDDS formulation using the design of experimentation approach held great potential as a possible alternative to traditional oral formulations of poorly soluble Bacoside A to improve solubility and bioavailability.

Downloads

Download data is not yet available.

References

Das A, Shanker G, Nath C, Pal R, Singh. A comparative study in rodents of standardized extracts of Bacopa monnierii and Ginkgo Biloba: anticholinesterase and cognitive enhancing activities. Pharmacol Biochem Behav 2002;73:893-900.

Tripathi Y. B, Bacopa monnierii Linn. As an antioxidant: mechanism of action. Indian J Exp Biol 1996;34:523-6.

Martis G, Rao A. Neuropharmacological activity of Herpestis monnierii. Fitoterapia 1992;63:399-404.

Russo A, Borrelli F. Bacopa monnieri, a reputed nootropic plant: an overview Elsevier; 2005. p. 305â€“17.

Azeem A, Rizwan M. Nanoemulsion components screening and selection, a technical note. AAPS PharmSciTech 2009;10:1-6.

Khaled Tahami. Preparation, characterization, and in vitro release of ketoprofen loaded alginate microspheres. Int J Appl Pharm 2014;6:9-12.

Pankaj Laddha, Vrunda Suthar, Shital Butani. Development and optimization of self-are micro emulsifying drug delivery of Domperidone. Braz J Pharm Sci 2014;50:90-100.

Sapra K, Saprab A, Singha SK. Self-emulsifying drug delivery system, a tool in solubility enhancement of poorly soluble drugs. Indo Glob J Pharm Sci 2012;2:313-32.

Sachan R, Khatri K, Kasture S. Self emulsifying drug delivery system approach for enhancement of bioavailability. Int J PharmTech Res 2010;2:1738-45.

Neeta Choudhary, DS Rathore. A review on self-emulsifying drug delivery system (SEDDS). Asian J Biochem Pharm Res 2014;4:109-18.

Zhao Yi, Wang C, Chow A, Ke Ren, Gong T, Zhang Z, et al. Self nano-emulsifying drug delivery system for oral delivery of Zedoary essential oil, Formulation and bioavailability studies. Int J Pharm 2010;383:170â€“7.

Sierra A, Naveros B. Design, and optimization of self-nano emulsifying drug delivery systems for enhanced dissolution of gemfibrozil. Int J Pharm 2012;431:161â€“75.

Negi L, Tariq M, Talegaonkar S. Nanoscale self-emulsifying oil based carrier system for improved oral bioavailability of camptothecin derivative by P-Glycoprotein modulation. Colloids Surf B 2013;111:346â€“53.

Zhou H, Wan J, Wu L, Yi T, Liu W, Xu H, et al. A new strategy for enhancing the oral bioavailability of drugs with poor water solubility and low liposolubility based on phospholipid complex and supersaturated SEEDS. Int J Pharm Sci Res 2013;8:1-12.

Kawakami K, Yoshikawa T, Moroto Y, Kanaoka E, Takahashi K, Nishihara Y. Microemulsion formulation for enhanced absorption of poorly soluble drugs I. prescription design. J Controlled Release 2002;81:65-74.

Linjie L, Xiujuan P, Wei Z, Siling W. Formulation design and in vitro evaluation of silymarin-loaded self-micro emulsifying drug delivery systems. Asian J Pharm Sci 2007;2:150-60.

Latika M Ingle, Vikrant P Wankhade, Tapar K. New approaches for development and characterization of SNEDDS. Int J Pharm 2013;3:7-14.

Mu L, Feng SS. Fabrication, characterization and in vitro release of paclitaxel (Taxol) loaded poly (lactic-co-glycolic acid) microspheres prepared by spray drying technique with lipid/cholesterol emulsifiers. J Controlled Release 2001;76:239-54.

Shantha KTR, Chawla S, Nachaegari SK, Singh SK, Srinivas NR. Validated HPLC analytical method with programme wavelength UV detection for simultaneous determination of DRF 4367 and phenol red in rat in situ intestinal perfusion study. J Pharm Biomed Anal 2005;38:173â€“9.

Youenang Piemi MP, Korner D, Benita S, Marty JP. Positively and negatively charged submicron emulsions for enhanced topical delivery of antifungal drugs. J Controlled Release 1999;58:177â€“87.