NEWER OPHTHALMIC IN SITU GEL OF MOXIFLOXACIN HYDROCHLORIDE: OPTIMIZATION USING BOX BEHNKEN STATISTICAL DESIGN

Mansi Dholakia; Richa Dave; Vaishali Thakkar; Hardik Rana; Mukesh Gohel; Nirav Patel

doi:10.22159/ijpps.2018v10i12.26979

Authors

Mansi Dholakia Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India
Richa Dave Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India
Vaishali Thakkar Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India
Hardik Rana Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India
Mukesh Gohel Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India
Nirav Patel Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India

DOI:

https://doi.org/10.22159/ijpps.2018v10i12.26979

Keywords:

Moxifloxacin hydrochloride (MH), Box-Behnken Design, Gelrite, HPMC K4M, Tamarind gum

Abstract

Objective: The present research work aims at describing the formulation and evaluation of the ocular delivery system of moxifloxacin hydrochloride (MH) based on the concept of ion sensitive in situ gelations.

Methods: In situ gel was prepared by a hot method using 0.6% of gelrite, 0.25% hydroxypropylmethylcellulose (HPMC K₄M) and 0.023% tamarind gum as bioadhesive polymers for sustained drug release. Optimization was done by Box Behnken Design with different concentration of gelrite (X₁), HPMC K₄M (X₂) and tamarind gum (X₃) as independent variables. In situ gel was optimized based on mucoadhesion index (Y1), Gel strength (Y2) and in vitro drug release (Y3). Influence of the quantitative variable on the dependent variable was predicted by a polynomial equation.

Results: Infrared spectroscopy excluded any interaction between drug and excipients. The selected independent variables significantly influenced the responses and were able to sustain the drug release. The prepared gel with a pH of 6.8 to 7.4 exhibited non-newtonian flow with no ocular irritation. The formulation remained stable with no change in pH and viscosity after 30 d of stability study.

Conclusion: Thus, moxifloxacin hydrochloride (MH) in situ gel is a viable alternative to a conventional delivery system with the properties of sustained drug release, prolonged ocular retention, and improved corneal penetration.

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