• SAHIDIN Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia
  • Nohong Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari 93232, Indonesia
  • Asrul Sani Faculty of Mathematics and Natural Sciences, Halu Oleo University, Kendari 93232, Indonesia
  • Marianti Anggrenimanggau Pharmacology, Faculty of Pharmacy, Hasanuddin University, Makassar 90245, Indonesia
  • Asep Sukohar Faculty of Medicine, Lampung University, Lampung 35145, Indonesia
  • Harto Widodo Medicinal Plants and Traditional Medicine Research and Development Centre (NIHRD), Jl. Raya LawuNo.10 Tawangmangu Central of Java 57792 Indonesia
  • Syarulnataqain Baharum Institute of Systems Biology, University Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia


P pulchrum Bl, Nil, 22-dien-3-one, Stigmasterol, Stigmasta-4, Ergosterol peroxide and DPPH


Objective: P. pulchrum grows abundantly in Kendari (Sulawesi Tenggara province, Indonesia).However, there is no report neither chemical contents nor biological activitiesof the plant. This project studiesthe isolation, structure elucidations, and radical scavenging activity evaluation of triterpene steroids from stems of P. pulchrum.

Methods: The isolation of the compounds was carried out by using chromatography method, i.e., vacuum liquid chromatography (VLC) and radial chromatography (RC) with silica gel as an adsorbent and various solvents as eluent. The compound structures were evaluated by spectroscopic data (FTIR and NMR data) and then the results were compared with the existing data from references. The antioxidant activity of these compounds was evaluated towards DPPH (1,1-diphenyl 2-picryl-hydrazyl).

Results: Four triterpene steroids; namely, (1) 6β-hydroxystigmasta-4,22-dien-3-one, (2) stigmasterol,(3) stigmasta-4,22-dien-3-one, and (4) ergosterol peroxide, were isolated and identified from stems of P. pulchrum Bl. The antioxidant activities of all compounds were indicated by IC50 value of the compounds. The values of IC50(µM) of6β-hydroxystigmasta-4,22-dien-3-one, stigmasterol, Stigmasta-4,22-dien-3-on,ergosterol peroxide, and vitamine C (standard) toward DPPH were obtained at233.4 ± 0.28; 372.3 ± 0.33; 144.80 ± 0.24; 1083.1 ± 0.38; and 68.9 ± 0.12, respectively.

Conclusions: We found that Stigmasta-4,22-dien-3-onewas the most active compound toward DPPH.


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Author Biography

SAHIDIN, Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia

Department of Pharmacy


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How to Cite

SAHIDIN, Nohong, A. Sani, M. Anggrenimanggau, A. Sukohar, H. Widodo, and S. Baharum. “RADICAL SCAVENGING ACTIVITY OF TRITERPENE STEROIDS FROM STEM OFPOLYGONUM PULCHRUM Bl”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 350-4, https://journals.innovareacademics.in/index.php/ijpps/article/view/1408.



Original Article(s)