A STUDY ON THE BIOACTIVE POTENTIAL OF FRESH AND DRIED SPROUTS OF COCOS NUCIFERA L.â€“AN IN VITRO AND IN-SILICO APPROACH
Keywords:Coconut sprouts, Phytochemical, Characterization, Antioxidant, Anti-inflammatory, In silico
Objective: The main objective of the study is to analyze the medicinal properties, by giving scientific validation to the existing bioactive compounds present in the coconut sprouts and recommending the coconut sprouts as a natural product for the treatment of bacterial infection leading to inflammations.
Methods: Phytochemical screening by qualitative and quantitative analysis of the primary and secondary constituents were carried out in aqueous and methanol extracts of the coconut sprouts (fresh and dried) using standard procedures. The phytochemicals were characterized using FT-IR, TLC and GC-MS analysis. Antibacterial activity was studied against several human pathogens by agar well diffusion method. Antioxidant activity using Reducing power, Hydrogen peroxide scavenging and DPPH radical scavenging methods and Anti-inflammatory activity through protein denaturation method were carried out. Further confirmation of the functional role of the phytoconstituent through in-silico docking was studied.
Results: The qualitative phytochemical screening revealed the presence of essential phytoconstituents. The quantitative analysis revealed the presence of maximum Carbohydrates (0.60Â±0.1 mg/g), Proteins (39Â±0.8 mg/ml), Flavonoids (0.28Â±0.1 mg QE/g) and Terpenoids (95Â±1.2 mg/g) in methanolic fresh coconut sprout extract with respective standards. Maximum zone of inhibitions were shown by Shigella flexneri, Salmonella typhi and Klebsiella pneumoniae. Antioxidant and anti-inflammatory studies proved the presence of therapeutic bioactive potential in the sample. Through in-silico analysis, docking studies were performed to confirm the functional role of the specific therapeutic phytochemical.
Conclusion: The fresh coconut sprouts are natural, economically potent food source for human health and can be a nutrient supplement with cost effective approach. The dried sprouts can also be recommended to the food industry for the large-scale production of nutrient-based foods with a quality check.
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