• Saranya V. T. K. Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008
  • S. Uma Gowrie Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008



Casuariana equisetifolia, bark, Phytochemicals, Antioxidant, Antibacterial, Anti-inflammatory, GC-MS


Objective: Casuarina equisetifolia is an important multipurpose exotic forest tree species widely cultivated in the coastal regions of Tamil Nadu that serves as a warehouse of essential secondary metabolites. Identification of these bioactive compounds in this forest tree species might lead to the discovery and development of a new drug to treat various diseases.

Methods: The present study was carried out with an objective to analyse the phytochemicals qualitatively and quantitatively. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was performed to evaluate the presence of various volatile compounds. An in vitro antibacterial, antioxidant and anti-inflammatory properties of aqueous and organic solvents of C. equisetifolia bark was studied.

Results: The preliminary qualitative screening revealed the presence of alkaloids, glycosides, carbohydrates, proteins, flavonoids, phenols, terpenoids, and tannins. The quantitative analysis revealed the presence of maximum phenols (71.2±0.51 mg/g), flavonoids (35.12±0.34 mg/g), tannins (77.59±0.21) and terpenoids (6%) in methanolic root extract with respective standards. Several peaks were obtained in the GC-MS analysis which indicates the presence of different secondary metabolites. Antibacterial activity showed a maximum zone of inhibition against Escherichia coli (23±0.24 mm) and Proteus vulgaris (23±0.32 mm). The antioxidant potential of various extracts was compared with the standard ascorbic acid. Anti-inflammatory activity was compared with standard diclofenac sodium and the extract showed activities significantly in a dose-dependent manner.

Conclusion: From this study, it is revealed that C. equisetifolia bark extract possesses efficient antibacterial property, the potential in scavenging free radical, effective antioxidant, powerful anti-inflammatory source that can be employed in the development of a novel drug to treat various diseases.


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Author Biographies

Saranya V. T. K., Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008

associate Professor, Department of plant Biology and Plant biotechnology, Ethiraj College for Women, Chennai 600008.

S. Uma Gowrie, Department of Plant Biology and Plant Biotechnology, Ethiraj college for women, Chennai 600008

Research Scholar,

Department of Plant Biology and Plant Biotechnology,

Ethiraj College for Women, Chennai


Fransworth NR. In bioactive compounds from plants. John and Wiley Co; 1990.

Sukanya SL, Sudisha J, Hariprasad P, Niranjana SR, Prakash HS, Fathima SK. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. Afr J Biotechnol 2009;8:6677-82.

Turnbull JW. The use of Casuarina equisetifolia for protection forests in China. In: Midgley SJ, Turnbull JW, Johnston RD. eds. Casuarina Ecology, Management and Utilization. Proceedings of the. 1st International Casuarina Workshop. Melbourne, Australia: CSIRO; 1983. p. 55-7.

Midgley SJ, Byron RN, Chandler FC, Ha Huy Thinh, Tran Vo Hung Son, Hoang Hong Hanh, Do plants need passports? A socio-economic study of the role of the exotic tree and other plant species in Quang Tri province, Vietnam. Canberra, Australia: CSIRO; 1997.

Shafi Thompson T, Alen Paiva, Greeshma G, Mohan, Jincy Das, Arys Suresh, et al. Phytochemical studies on C. equisetifolia and investigation of its effect against the oral pathogen. Int J Pharma Sci Res 2012;90:24.

Kishore DV, Rahman R. Spasmolytic activity of Casuarina equisetifolia bark extract. Int J Pharm Sci Res 2012;3:1452-6.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;299:152-78.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colourimetric methods. J Food Drug Anal 2002;10:178-82.

Sadasivam S, Manickam A. Biochemical methods for agricultural sciences. Wiley eastern limited, New delhi; 1992. p. 6-7, 188-9.

Tejavathi DH, Jayashree DR. Phytochemical screening of selected medicinal herbs inoculated with arbuscular mycorrhizal fungi. Int J Biol Pharm Allied Sci 2013;2:2090-106.

Blios MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199–200.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003â€8.

Oyaizu M. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Japan J Nutr 1986;44:307â€14.

Smirnoff N, Cumbes QJ. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 1989;24:1057–60.

Pilar Prieto, Manuel Pineda, Miguel Aguilar. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharma Pharmacol 1968;20:169-74.

Nusrat J Bristy, Mohammad F Islam, Sharif M Anisuzzaman, Mohammad N Alam. Antioxidant activity of the water extracts of leaves, root barks, barks of Casuarina littorea. Aust J Basic Appl Sci 2014;8:419-26.

Baravkar A, Kale A, RN Patil, Sawant SD. Pharmaceutical and biological evaluation of formulated cream of methanolic extract of Accacia nilotica. Res J Pharm Technol 2008;1:481-3.

S Kumar, A Mishra, AK Pandey. Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complementary Alternative Medicine 2013;13:120.

S Kumar, AK Pandey. Phenolic content, reducing power and membrane protective activities of Solanum xanthocarpum root extracts. Vegetos 2013;26:301-7.

Shahrzad S, Aoyagi K, Winter A, Koyama A, Bitsch I. Pharmacokinetics of gallic acid and its relative bioavailability from tea in healthy humans. J Nut 2001;131:1207-10.

Park HH, Lee S, Son HY, Park SB, Kim MS, Choi EJ, et al. Flavonoids inhibit histamine release and expression of pro-inflammatory cytokines in mast cells. Arch Pharm Res 2008;31:1303–11.

RI Okoli, AA Turay, JK Mensah, AO Aigbe. Phytochemical and antimicrobial properties of four herbs from Edo State, Nigeria. Report Opinion 2009;1:67-73.

Yondu J, Dongsmo G, Marie C, Wabo J, Olivia T, Jules-Roger, Blaise M. In vitro antioxidant potential and phytochemical constituents of three Cameroonian medicinal plants. Pharmacol Online 2009;10:648-57.

Manisuthisakul P, Suttajit M, Pongsawatmanit R. Assesment of phenolic content and free radical scavenging capacity of some Thai indigenous plants. Food Chem 2007;100:1409-18.

Park HH, Lee S, Son HY, Park SB, Kim MS, Choi EJ. Flavonoids inhibit histamine release and expression of pro-inflammatory cytokinensis in mast cells. Arch Pharm Res 2008;31:1303-11.

Karamaæ MA, Kosiñska, Pegg RB. Comparison of radical-scavenging activities of selected phenolic acids. Pol J Food Nutr Sci 2005;2:165-70.

Kaur S, Michael H, Arora S, Harkonen PL, Kumar S. The in vitro cytotoxic and apoptotic activity of Triphala-an Indian herbal drug. J Ethnopharm 2005;97:15-20.

Isosorbide Dinitrate/Mononitrate. The American Society of Health-System Pharmacists; 2016.

Hecker M, Hollert H, Cooper R, Vinggaard AM, Akahori Y, Murphy M, et al. The OECD validation program of the H295R Steroidogenesis Assay for the identification of in vitro inhibitors and inducers of testosterone and estradiol production. Phase 2: inter-laboratory pre-validation studies. Environ Sci Pollut Res 2007;14:23–30.

Alok Prakash, Suneetha V. Punica granatum (Pomegranate) rind extract as a potent substitute for L-ascorbic acid with respect to the antioxidant activity. Res J Pharm Biol Chem Sci 2014;2:597-603.

Nehad M Gumgumjee, Abdulrahman S Hajar. Antimicrobial efficacy of Casuarina equisetifolia extracts aginst some pathogenic microorganism, Journal of Medicinal Plants Research 2012;6:5819-25.

Moreno S, Scheyer T, Romano C, Vojnov A. Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Res 2006;40:223-31.

Kamalakannan P, Kavitha R, Elamathi R, Deepa T, Sridhar S. Study of the phytochemical and antimicrobial potential of methanol and aqueous extracts of aerial parts of Elephantopus scaber L. Int J Curr Pharm Res 2012;4:18-21.

Sharma BL, Daulat Singh, Santosh Sharma K, Afzal Hashmi, Arjun Singh, Anil Bansal. Studies on some primary metabolite’s extraction and Quantification in different plant parts of selected cassia species. Asian J Pharm Clin Res 2013;6:309-14.

AN Aher, SC Pal, SK Yadav, UK Patil, S Bhattacharya. Antioxidant activity of isolated phytoconstituents from Casuarina equisetifolia frost (Casuarinaceae). J Plant Sci 2009;4:15-20.

Lucio M, Nunes C, Gaspar D, Ferreira H, Lima JLFC, Reis S. Antioxidant activity of vitamin E and Trolox: understanding of the factors that govern lipid peroxidation studies in vitro. Food Biophysics 2009;4:312-20.

Gulcin IS, Bey Demir HA, Alici M, Buyukokuroglu ME. In vitro antioxidant proper tog morphine. Pharmocologic used to manage parasitic diseases. Research 2004;2:59-66.

Cotelle N. Role of flavonoids in oxidative stress. Curr Tropics Med Chem 2001;1:569-90.

B Fuhrman, S Buch, J Vaya. Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation: in vitro and ex vivo studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr 1997;66:267–75.

Ryszard Amarowicz. Tannins: the new natural antioxidants? Eur J Lipid Sci Technol 2007;109:549–51.

M Karamac, A Kosinska, R Amarowicz. Chelating of Fe(II), Zn(II) and Cu(II) by tannin fractions separated from hazelnuts, walnuts and almonds. Bromat Chem Toksykol 2006;10:257–60.

YJ Zhang, DL DeWitt, S Murugesan, MG Nair. Novel lipi-peroxidation and cyclooxygenase inhibitory tannins from Picrorhiza kurrora seeds. Chem Biodiver 2004;1:426–41.

Grassmann J. Terpenoids as plant antioxidants. Vitam Horm 2005;72:505-35.

Yousra Shafiq, Baqir Shyum Naqvi, Ghazala H Rizwani, Muhammed Usman, Barkat Ali Shah, Muhammad Aslam, et al. the Anti-acne activity of Casuarina equisetifolia bark extract: a randomized clinical trial. Bangladesh J Pharmacol 2014;9:337-41.

Middleton EJr, Kandaswami C, Theoradies TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol Rev 2000;52:673–751.

Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents. Inflamm Allergy Drug Targets 2009;8:229–35.

Gonzalez R, Ballester I, Lopez-Posadas R, Suarez MD, Zarzuelo A, Martinez-Augustin O, et al. Effects of flavonoids and other polyphenols on inflammation. Crit Rev Food Sci Nutr 2011;51:331-62.

Sreedevi1 K, Vijayalakshmi R, Venkateswari. Phytochemical evaluation of Punica granatum L. leaf extract. Int J Curr Pharm Res 2017;9:14-8.

Dependra Chamlagai, Bisu Singh. Study of in vitro anti-inflammatory activity of ethnomedicinal plants of sikkim Viscum articulatum and Acorus calamus. Asian J Pharm Clin Res 2016;9:119-22.



How to Cite

K., S. V. T., and S. U. Gowrie. “PHYTOCHEMICAL ANALYSIS AND IN VITRO STUDIES ON ANTIBACTERIAL, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES USING CASUARINA EQUISETIFOLIA BARK EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 1, Jan. 2018, pp. 118-25, doi:10.22159/ijpps.2018v10i1.22188.



Original Article(s)