CHEMICAL COMPOSITION AND CHARACTERIZATION STUDIES OF CASSIA AURICULATA FLOWER EXTRACT
Keywords:
n-hexane, Ethyl acetate, Flavonoid, FT-IR, PhenolAbstract
Objectives: The present study, aimed to determine and characterize the chemical constituents of Cassia auriculata flower extract by qualitative, quantitative and analytical techniques.
Methods: Preliminary Phytochemical, total flavonoid and phenol content was determined in the methanolic extract of C.auriculata (CAFMEt) using standard methods. C-18 silica gel based column chromatography was used to purify CAFMEt using n-hexane, ethyl acetate and methanol and fraction identified by thin layer chromatography. GC-MS and FT-IR techniques used to characterize the lead fraction.
Results: CAFMEt showed the presence of flavonoid and Phenols in a significant amount. Three fractions was collected from column chromatography viz., Fraction 1-3 (n-hexane: yellow) was 2.5mg, (ethyl acetate: light orange) 1.8mg and (methanol: light green) 5.67mg respectively. TLC indicated n-hexane has higher refractive factors 0.457 at yellow band and ethyl acetate fraction has 0.329 at light orange band. 14 chemical constituents were identified by GC-MS included alkanes, alcohol, esters and hydrocarbons. The major peak showed the presence of 4-(4-methylphenoxy) phenol at 22.53%. Infra red spectra revealed the presence of phenolic groups in hexane fraction.
Conclusion: Further studies will be carried out the pharmacological potential of n-hexane fractions of flowers of C.auriculata.
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References
Sirajuddin SM, Anthropological I. All India Anthropometric Survey:Lakshadweep of India. J Chromatography A 2006.
Gurudeeban S. Studies on type 2 diabetes and formulation of tablets from DNA bar-coded Rhizophora apiculata Blume derived Glycosin on Streptozotocin-induced diabetic rats: in vivo, in silico and molecular approaches, Ph.D. Thesis, Annamalai University, Tamil Nadu, India, 2013.
Kuhnau J. The flavonoids. A class of semi-essential food components:Their role in human nutrition. In:Bourne GH, editors. World Review Nutrition Diet. Basel: Switzerland; 1976.117–120.
Strack D, Dey PM, Harborne JB, London UK. Phenolic Metabolism1997. 387-416.
Savithramma N, Rao M, Bhumi G, J. Linga Phytochemical screening of Thespesia populnea (L.) Soland and Tridax procumbens L. J Pharm Res 2011;3:28-34.
Chen H, Zuo Y, Deng Y. Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography. Journal Chromatography A 2001;913(1-2):387-95.
Thulasi G, Amsaveni V, Esbl, I. UT, J. Antibacterial Activity of Cassia auriculata against E. J Coli From int Res 2012;3:24-9.
Anandan A, Eswaran R, Doss A, Sangeetha G, Anand SP, A. Chemical Compounds Investigation of Cassia auriculata leaves-Folklore Medicinal Plant. Bull Environ Pharmacol Life Sci 2011;1:20-3.
Chauhan KN, Patel MB, Valera HR, Patil SD, Surana SJ, J. Hepatoprotective activity of flowers of Cassia auriculata R. Br against paracetamol induced liver injury Rem 2009;9:85-90.
Raj J, Peter M, Joy V, J. Yesu Paul John Chemical compounds investigation of Cassia auriculata seeds:A potential folklore medicinal plant. J Asian Sci 2012;2:187-92.
Purushotham KN, Annegowda HV, Sathish NK, Ramesh B, Mansor SM. Evaluation of phenolic content and antioxidant potency in various parts of Cassia auriculata L.:a traditionally valued plant. Pakistan J Biol Sci:PJBS 2014;17(1):41-8.
Rastogi RP, Mehrotra BN, New C. Compendium of Indian Medicinal plants. Central Drug Research Institute Lucknow and National Institute of Science India 1994.
Evans WC. Trease and Evans Pharmacognosy, 14th ed1997.
Chang C, Yang M, Wen H, Chem J, J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Drug Anal 2002;10:178-82.
Ausloos P, Clifton CL, Lias SG, Mikaya AI, Stein SE, Tchekhovskoi DV, et al. The critical evaluation of a comprehensive mass spectral library. J Am Soc Mass Spectrom 1999;10(4):287-99.
Harborne JB. In:Introduction to Ecological Biochemistry, 2nd ed1982.
Sachin C, Amit K, J. Phytochemical Analysis and Assessment of In-vitro Anthelmintic Activity of Cassia auriculata Linn leaves. J Am Clin Therapeut 2014;2:161-7.
Rota MC, Herrera A, Martinez RM, Sotomayor JA, Jordan MJ. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. J Food Control 2008;19:681-7.
Sukumaran S, Kiruba S, Mahesh M, Nisha SR, Miller PZ, Ben CP, et al. Phytochemical constituents and antibacterial efficacy of the flowers of Peltophorum pterocarpum (DC.) Baker ex Heyne. J Asian Pac J Trop Med 2011;4(9):735-8.
Thambidurai M, Rajesh P, Balamurugan B, Kannan VR, J. In vitro antioxidant and anti-microbial of study on Cassia auriculata L. J Int Biol Sci 2010;2:1-7.
Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pac J Trop Med 2011;4(9):717-21.
Gurudeeban S, Satyavani K, Rajamanickam E, Ramanathan T, Balasubramanian T. Evaluation of Chemical composition and cytotoxicity Effect of Rhizophora mucronata. J Inventi Rapid Med Chem 2013;4:1-5.
Hossaina MA, Wafa AS, Afaf A, W. M, J. Toubia Al, Riyamia Al, Identification and characterization of chemical compounds indifferent crude extracts from leaves of Omani neem. J Uni Sci 2013;7:181-8.
Victor CG, Jaime PM. Identification of volatiles from Ceratitis capitata (Wiedemann) (Diptera:Tephritidae) by means of thermal desorption of solid samples coupled to GC-MS. IOBC WPRS Bull 1999;22:21-25.