PHYCHEMISTRY, PHYTOCHEMICAL, PHARMACOLOGICAL AND MOLECULAR STUDY OF ZINGIBER OFFICINALE ROSCOE: A REVIEW
DOI:
https://doi.org/10.22159/ijpps.2017v9i11.19613Keywords:
Zingiber officinale, gingerol, shogaol, molecular marker, NilAbstract
The rhizomes of Zingiber officinale Roscoe (Zingiberaceae), commonly known as ginger, is one of the most widely used spice and condiment. It is also an integral part of many traditional medicines and has been extensively used in Chinese, Ayurvedic, Tibb-Unani, Srilankan, Arabic, and African traditional medicines, since antiquity, for many unrelated human ailments including common colds, fever, sore throats, vomiting, motion sickness, gastrointestinal complications, indigestion, constipation,  gastritis, epigastric discomfort, gastric ulcerations, indigestion, nausea  vomiting etc. and scientific studies have validated the ethnomedicinal uses. The chemistry, phytochemical, pharmacological and molecular studies of Z. officinale is reviewed. This review was compiled to provide consolidated information covering different aspects of the plant, to provide a basis on which to plan future studies and to promote sustainable use of Z. officinale.
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Berg LR. Introductory Botanyâ€: Plants, People and the Environment. Ft. Worth: Saunders College Pubication 1997: 466.
Rout GR, Das P, Goel S. Raina, SN. Determination of genetic stability of micropropagated plants of ginger using Random Amplified Polymorphic DNA (RAPD) markersâ€, Botanical Bull. Acad. Sinic 1998; 39: 23-37.
Simpson MG. Plants Systematic, 1st Edn. Elsevier, Academic Press, Amsterdam, ISBN: 10: 0126444609 2006; pp: 590.
Awang DVC. Ginger.Canâ€, Pharm 1992; 125:309-311.
Combest WL. Herbal pharmacy.Ginger natural medicine comprehensive 2007.
Sheetal G, Jamuna P. Studies on Indian green leafy vegetables for their antioxidants activity. Plant Hum Nutr 2009; 64: 39–45.
Wang WH, Wang ZM. Studies of commonly used traditional medicine-gingerâ€, Zhongguo Zhong Yao Za Zhi 2005; 30:1569–1573.
Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, et al. Health benefits of herbs and spices: the past, the present, the future, Med J Aust 2006; 185 (Suppl. 4): S4–S24.
Afzal M, Al-Hadidi D, Menon M, Pesek J, Dhami MS. Ginger: an ethnomedical, chemical and pharmacological review. Drug Metab Drug Interact 2001; 18: 159–190.
Chrubasik S, Pittler MH, Roufogalis BD. Zingiberis rhizoma: a comprehensive review on the ginger effect and efficacy profiles Phytomedicine 2005; 12:684–701.
Connell DW. The pungent principles of ginger and their importance in certain ginger products. Food Tech 1969; Aust. 21: 570-575.
Govindarajan VS. Ginger-chemistry, technology, and quality evaluation. Part 1. Crit. Rev. Food Sci & Nutr 1982;17: 1-96.
Connell DW, Sutherland MD. A re-examination of gingerol, shogaol and zingerone, the pungent principles of ginger (Zingiber officinale Roscoe). Aust J Chem 1969; 22:1033-1043.
Denniff P, Macleod I, Whiting DA. Synthesis of the (±)-[n]-gingerols (pungent principles of ginger) and related compounds through regioselective aldol condensations: relative pungency assays. J Chem Soc Perkin Transactions 1981; 82-87.
Hikino H, Takahashi H, Sakurai Y, Takemoto T, Bhacca NS. Structure of zederone. Chem Pharma Bullet 1966; 14:550-551.
He XG, Bernart MW, Lian LS, Lin LZ. High-performance liquid chromatography-electrospray mass spectrometric analysis of pungent constituents of ginger. J Chromat 1998; 796:327-334.
Kikuzaki H. Ginger for drug and spice purposes, In: Herbs, Botanicals and Teas, edired by Mazza GO, Dave B. Technomic Publishing Co. Lancaster 2000; 75-105.
Jolad SD, Lantz, RC, Chen GJ, Bates RB, Timmermann BN. Commercially processed dry ginger (Zingiber officinale): composition and effects on LPS-stimulated PGE2 production, Phytochem 2005; 66:1614-1635.
Wohlmuth H. Phytochemistry and pharmacology of plants from the ginger family, Zingiberaceae. Ph.D. Thesis. Southern Cross University 2008; 47-53.
Lawrence BM. Progress in essential oils. Perfumer & Flavorist 1995; 20:49-59.
Lawrence BM. Progress in essential oils. Perfumer & Flavorist 2000; 25: 46-57.
Colleen NA, Salmon YA. Shaw B, Hibbert S, Green C, Smith AM, Lawrence ADW. Characterisation of cultivars of Jamaican ginger (Zingiber officinale Roscoe) by HPTLC and HPLC. Food Chemistry 2012; 131:1517–1522.
Khan I, Pandotra P, Gupta AP, Sharma R, Gupta BD, Dhar JK, Ram G, Bedi YS, Gupta S. RP-thin layer chromatographic method for the quantification of three gingerol homologs of ultrasonic-assisted fresh rhizome extracts of Zingiber officinale collected from North Western Himalayas. J Sep Sci 2010 Mar., 33(4-5):558-63.
Nilesh P, Pai S, Nimbalkar M, Dixit G.RP-HPLC analysis of phenolic antioxidant compound 6-gingerol from different ginger cultivars. Food Chemistry 2011; 126: 1330–1336.
Mahdi HJ, Andayani R, Ishak.Metabolic Fingerprinting of Three Malaysian Ginger (Zingiber officinale Roscoe) Using Gas Chromatography-Mass Spectromertry. Amer J App Sci 2010; 7 (1): 17-23.
Schwertner HA, Rios DC.High-performance liquid chromatographic analysis of 6- gingerol, 8-gingerol, 10-gingerol, and 6-shogaol in ginger-containing dietary supplements, spices, teas, and beverages. J Chromatogr B 2007; 856:41–47.
Rai S, Mukherjee K, Mainak MM, Wahile A, Saha BP, Mukherjee PK. Determination of 6-gingerol in ginger (Zingiber officinale) using high-performance thin-layer chromatography. J Sep Sci 2006; 29 (15):2292–2295.
Jiang H, Xie Z, Koo HJ, McLaughlin SP, Timmermann BN, Gang DR. Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: tools for authentication of ginger (Zingiber officinale Rosc.). Phytochem 2006; 67: 1673–1685.
Ashraf K, Mujeeb M, Ahmad A, Ahmad S, Ahmad N, Amir M. Determination of Gingerols in Ginger by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. Anal Lett 2014; 47(12):2120-2128.
He XG, Bernart MW, Lian LS, Lin LZ. High-performance liquid chromatography-electrospray mass spectrometric analysis of pungent constituents of ginger J Chromat 1998; 796 :327-334.
Ashraf K, Mujeeb M. Determination and validation of 6-gingerol in ginger varieties of Indian subcontinent by hplc method. World Jour of Pharmacy and Pharmaceut Sci 2016; 5 (9):1297-1305.
Ashraf K, Shah SAS, Mujeeb M. Determination of 10-gingerol in indian ginger by validated hptlc method of samples collected across subcontinent of India. Int J Pharmacy and Pharmac Sci 2016; 8 (12): 190-193.
Alam P. Densitometric HPTLC analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams. Asian Pac J Trop Biomed 2013; 3(8):634-8.
Park SY, Jung MY. UHPLC-ESI-MS/MS for the Quantification of Eight Major Gingerols and Shogaols in Ginger Products: Effects of Ionization Polarity and Mobile Phase Modifier on the Sensitivity. J Food Sci 2016; 81(10): 2457-2465.
Miyoshi N, Nakamura Y, Ueda Y, Abe M, Ozawa Y, Uchida K et al. Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells Cancer Lett 2003; 199 (2): 113-119.
Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol 2006; 71(10):1397-421.
Shukla Y, Singh M. Cancer preventive properties of ginger. A brief review. Food Chem Toxicol 2007; 45:683–690.
Ishiguro K, Ando T, Maeda O, Ohmiya N, Niwa Y, Kadomatsu K, et al. Ginger ingredients reduce viability of gastric cancer cells via distinct mechanisms. Biochem Biophys Res Commun 2007;362:218 23.
Sung B, Jhurani S, Ahn KS, Mastuo Y, Yi T, Guha S, et al. Zerumbone down regulates Chemokine receptor CXCR4 expression leading to inhibition of CXCL 12induced invasion of breast and pancreatic tumor cells. Cancer Res 2008;68:8938–44.
Brown AC, Shah C, Liu J, Pham JT, Zhang JG, Jadus MR. Ginger's (Zingiber officinale Roscoe) inhibition of rat colonic adenocarcinoma cells proliferation and angiogenesis in vitro. Phytother Res 2009;23:640–5.
Kim M, Miyamoto S, Yasui Y, Oyama T, Murakami A, Tanaka T. Zerumbone, a tropical ginger sesquiterpene, inhibits colon and lung carcinogenesis in mice. Int J Cancer 2009;124:264 71.
Lee HS, Seo EY, Kang NE, Kim WK. [6] Gingerol inhibits metastasis of MDAMB231 human breast cancer cells. J Nutr Biochem 2008;19:313–9.
Sung B, Murakami A, Oyajobi BO, Aggarwal BB. Zerumbone abolishes RANKL induced NFkappaB activation, inhibits osteoclastogenesis, and suppresses human breast cancerinduced bone loss in athymic nude mice. Cancer Res 2009;69:1477–84.
Yagihashi S, Miura Y, Yagasaki K. Inhibitory effect of gingerol on the proliferation and invasion of hepatoma cells in culture. Cytotechnology 2008;57:129–36.
Habib SH, Makpol S, Abdul Hamid NA, Das S, Ngah WZ, Yusof YA. Ginger extract (Zingiber officinale) has anticancer and anti-inflammatory effects on ethionineinduced hepatoma rats. Clinics (Sao Paulo) 2008;63:807–13.
Nigam N, Bhui K, Prasad S, George J, Shukla Y. [6] Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells. Chem Biol Interact 2009;181:77–84
Wang YH, Gou Xj, Yan WZ, Ma XJ. Yang Y. Hairy roots induction of Phellodenderon Chinees and production of its active constituents. Zhongguo Zhong Yao Za Zhi 2006; 31(22):1853-56.
Nurtjahja-Tjendraputra E, Ammit AJ, Roufogalis BD, Tran VH, Duke CC. Effective anti-platelet and COX-1 enzyme inhibitors from pungent constituents of ginger. Thromb Res 2003; 111(4-5):259-265.
Verma SK, Singh J, Khamesra R, Bordia A. Effect of ginger on platelet aggregation in man. Indian. J Med Res 1993; 98: 240-242.
Lumb AB. Mechanism of antiemetic effect of ginger. Anaesthesia 1993; 48(12): 11-18.
Bhattarai S, Tran VH, Duke CC.The stability of gingerol and shogaol in aqueous solutions. J Pharm Sci 2001; 90(10):1658-1664.
Huang Q, Iwamoto M, Aoki S. Anti-5-hydroxytryptamine3, effect of galanolactone, diterpenoid isolated from ginger. Chem Pharm Bull 1991; 39(2):397-399.
Vellacott ID, Cooke EJ, James CE. Nausea and vomiting in early pregnancy. Int J Gynaecol Obstet. 1988; 27(1):57–62.
Lacroix R, Eason E, Melzack R. Nausea and vomiting during pregnancy: a prospective study of its frequency, intensity and patterns of change. Am J Obstet Gynecol. 2000; 182(4):931–7.
McCarthy FP, Lutomski JE, Greene RA. Hyperemesis gravidarum: current perspectives. Int J Womens Health. 2014; 6:719–25.
Yamahara J, Miki K, Chisaka T, Sawada T, Fujimura H, Tomimatsu T, Nakano K, Nohara T. Cholagogic effect of ginger and its active constituents. J Ethnopharmacol 1985; 13(2): 217- 225.
Srivastava KC. Aqueous extracts of onion, garlic and ginger inhibit platelet aggregation and alter arachidonic acid metabolism. Biomed Biochim Acta 1984; 43: 335-S346.
Chrubasik S, Pittler MH, Roufogalis BD. Zingiberis rhizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomed 2005; 12(9): 684-701.
Habib SH, Makpol S, Abdul Hamid NA, Das S, Ngah WZ, Yusof YA. Ginger extract (Zingiber officinale) has anticancer and anti-inflammatory effects on ethionineinduced hepatoma rats. Clinics (Sao Paulo) 2008;63:807–13.
Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol 2006;71:1397–421.
Lantz RC, Chen GJ, Sarihan M, Sólyom AM, Jolad SD, Timmermann BN. The effect of extractsfrom ginger rhizome on inflammatory mediator production. Phytomedicine 2007;14:123–8.
Ma J, Jin X, Yang L, Liu ZL. Diarylheptanoids from the rhizomes of Zingiber officinale Phytochem 2004; 65(8):1137-1143.
Sepahvand R, Esmaeili-Mahani S, Arzi A, Rasoulian B, Abbasnejad M. Ginger (Zingiber officinale Roscoe) elicits antinociceptive properties and potentiates morphine-induced analgesia in the rat radiant heat tail-flick test. J Med Food; 2010 13(6):1397-401.
Shukla Y, Singh M. Cancer preventive properties of ginger: A brief review. Food Chem Toxicol. 2007;45:683–90.
Nigam N, Bhui K, Prasad S, George J, Shukla Y. [6] Gingerol induces reactive oxygen speciesregulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells. Chem Biol Interact 2009;181:77–84.
Dugasani S, Pichika MR, Nadarajah VD, Balijepalli MK, Tandra S, Korlakunta JN. Comparative antioxidant and anti-inflammatory effects of [6]gingerol, [8]gingerol, [10]gingerol and [6]shogaol. J Ethnopharmacol 2010;127:515–20.
ElSharaky AS, Newairy AA, Kamel MA, Eweda SM. Protective effect of ginger extract against Bromobenzeneinduced hepatotoxicity in male rats. Food Chem Toxicol 2009;47:1584 90.
Ahmed RS, Seth V, Banerjee BD. Influence of dietary ginger (Zingiber officinales Rosc) on
antioxidant defense system in rat: Comparison with ascorbic acid. Indian J Exp Biol 2000;38:604–6.
Nigam N, Bhui K, Prasad S, George J, Shukla Y. [6] Gingerol induces reactive oxygen species regulated mitochondrial cell death pathway in human epidermoid carcinoma A431 cells. Chem Biol Interact 2009;181:77–84.
Rhode J, Fogoros S, Zick S, Wahl H, Griffith KA, Huang J, et al. Ginger inhibits cell growth and modulates angiogenic factors in ovarian cancer cells. BMC Complement Altern Med 2007;7:44.
Mallikarjuna K, Sahitya Chetan P, Sathyavelu Reddy K, Rajendra W. Ethanol toxicity: Rehabilitation of hepatic antioxidant defense system with dietary ginger. Fitoterapia 2008;79:174–8.
Bhandari U, Sharma JN, Zafar R.The protective action of ethanolic ginger (Zingiber officinale) extract in cholesterol fed rabbits. J Ethnopharmacol 1998; 61 (2):167- 171.
Fuhrman B, Rosenblat M, Hayek T, Coleman R, Aviram M. Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apolipoprotein E-deficient mice. J Nutr 2000;130(5):1124-1131.
Ghayur and Gilani. Ginger lowers blood pressure through blockade of voltagedependent
calcium channels. J. Cardiovasc. Pharmacol 2005; 45 (2005):74–80.
Harborne JB. Phytochemical Methods Chapman and Hall, London 1984:1–36
Williamson EM, Okpako DT, Evans FJ. Pharmacological Methods in Phytotherapy Research Wiley. Chichester 1996:15–24
Pancho LR, Kimura I, Unno R, Kurono M, Kimura M. Reversed effects between crude and processed ginger extracts on PGF2 alphainduced contraction in mouse mesenteric veins Jpn J Pharmacol 1989;50:243–246.
Weidner MS, Sigwart V.The safety of a ginger extract in the rat J. Ethnopharmacol 2000;7: 513–520.
Thomson M, Al Qattan KK, Al Sawan SM, Alnaqeeb MA, Khan I, Ali M. The use of ginger (Zingiber officinale Rosc.) as a potential anti-inflammatory and antithrombotic agent. Prostagl Leukot Essent Fatty Acids 2002; 67 (6): 475-478.
Srivastava KC. Aqueous extracts of onion, garlic and ginger inhibit platelet aggregation and alter arachidonic acid metabolism.Biomed Biochim Acta 1984; 43: 335-S346.
Arunlakhshana O, Schild HO: Some quantitative uses of drug antagonists. Br J Pharmacol 1959; 14:48–58.
Gilani AH, Cobbin LB. Cardioselectivity of himbacine: a muscarinic receptor antagonist. Naunyn-Schmiedeberg’s. Arch Pharmacol 1986; 332:16–20.
Kerr PM, Hillier K, Wallis RM, Garland CJ. Characterization of muscarinic receptors mediating contractions of circular and longitudinal muscle of human colon. Br J Pharmacol 1995; 115:1518–1524.
. Morisset J, Geoffrion L, Larose L, Lanoe J, Poirier GG. Distribution of muscarinic receptors in the digestive tract organs. Pharmacology 1981; 22:189–195.
Johnson PJ, Bornstein JC, Yuan SY, Furness JB. Analysis of contribution of acetylcholine and tachykinins to neuro-neuronal transmission in motility reflexes in the guinea pig ileum. Br J Pharmacol 1996; 118:973–983.
Nadkarni KM. Zingiber officinale. In Indian Materia Medica. Bombay, Popular Prakashan, 1976; 1308–1315
Kapoor LD. Zingiber officinale. In Handbook of Ayurvedic Medicinal Plants. Boca Raton, FL, CRC Press, 1990; 341–342.
Tyler VE. Ginger. In The Honest Herbal, 3rd ed. New York, Pharmaceutical Products Press 1993;147–148.
Westerterp-Plantenga M, Diepvens K, Joosen AM, Berube-Parent S, Tremblay A. Metabolic effects of spices, teas, and caffeine. Physiol Behav 2006; 89(1): 85-91.
Funk JL, Frye JB, Oyarzo JN, Timmermann BN. Comparative Effects of Two Gingerol-Containing Zingiber officinale Extracts on Experimental Rheumatoid Arthritis. J Nat Prod 2009; 72:403-407.
Bordia A, Verma SK, Srivastava KC. Effect of ginger (Zingiber officinale Rosc.) and fenugreek (Trigonella foenumgraecum L.) on blood lipids, blood sugar and platelet aggregation in patients with coronary artery disease. Prostagl Leukot Essent.Fatty Acids 1997; 56(5):379- 384.
Miri P, Bae J, Lee DS. Antibacterial activity of [10]-gingerol and [12]- gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res 2008; 22:1446-1449.
Onyeagba RA, Ugbogu OC, Okeke CU and Iroakasi O. Studies on the antimicrobial effects of garlic (Allium sativum Linn), ginger (Zingiber officinale Roscoe) and lime (Citrus aurantifolia Linn). African Journal of Biotechnology 2004; 3 (10):552-554.
Malu SP, Obochi GO, Tawo EN, Nyong B.E. Antibacterial activity and medicinal properties of ginger (Zingiber officinale). Global Journal of Pure and Applied Sciences 2009; 15:3-4.
Kim JK, Kim Y, Na KM, Surh YJ, Kim TY.[6]-Gingerol prevents UVBinduced ROS production and COX-2 expression in vitro and in vivo. Free Rad Res 2007; 41: 603-614.
Beg T, Siddiqe YS, Ara G, Gupta J, Afzal M. Antigenotoxic Effect of Genistein and gingerol on genotoxicity induced by norethandrolone and oxandrolone in cultured human lymphocytes.Int. J Pharmacol 2008; 4:177-183.
Rout GR, Das P, Goel S, Raina SN. Determination of genetic stability of micropropagated plants of ginger using Random Amplified Polymorhic DNA (RAPD) markers. Bot Bull Acad.1998; Sin.39 (1): 23–27.
Palai SK, Rout GR. Identification and genetic variation among eight varieties of ginger by using random amplified polymorphic DNA markers. Plant Biotech 2007; 24:417–420.
Ravindran PN, Nirmal BK. (Eds.). Ginger: The Genus Zingiber. CRC Press, New York. 2005; pp. 15–85.
Ashraf K, Ahmad A, Chaudhary A, Mujeeb M, Ahmad S, Amir M, Mallick N.Genetic diversity analysis of Zingiber Officinale Roscoe by RAPD collected from subcontinent of India. Saudi J Biol Sci 2014; 21:159–165.
Kizhakkayil J, Sasikumar B. Genetic diversity analysis of ginger (Zingiber officinale Rosc.) germplasm based on RAPD and ISSR markers. Sci Hort 2011; 125:73–76.
Jatoi SA, Kikuchi A, Mimura M, Yi SS, Watanabe KN. Relationships of Zingiber species and genetic variability assessment in ginger (Zingiber officinale) accessions from ex-situ gene bank, on-farm and rural markets. Breed. Sci 2008; 58:261–270.
Mimura M, Yasuda K, Yamaguchi H. RAPD variation in wild, weedy and cultivated adzuki beans in Asia. Genet Resour Crop Evol 2000; 47: 603–610.
Da Costa FR, Pereira TNS, Vitoria AP, De Campos KP, Rodrigues R, Da Silva DJH, Pereira MG.Genetic diversity among Capsicum accessions using RAPD markers,Crop Breed Appl Biotech 2006; 6:18–23.
Jiang H, Xie Z, Koo HJ, McLaughlin SP, Timmermann BN, Gang DR. Metabolic profiling and phylogenetic analysis of medicinal Zingiber species: tools for authentication of ginger (Zingiber officinale Rosc.). Phytochem 2006; 67:1673–1685.
Nayak S, Naik PK, Acharya L, Mukherjee AK, Panda PC, Das P. Assessment of genetic diversity among 16 promising cultivars of ginger using cytological and molecular markers. Z. Naturforsch 2005; 60c: 485–492.
Mohd MA, Ibrahim H, Khalid N. Differentiation of three vaieties of Zingiber officinale Rosc. by RAPD fingerprinting. Malaysian J Sci 2004; 23:135–139.
Wahyuni S, Xu DH, Bermawie N, Tsunematsu H, Ban T. Genetic relationships among ginger accessions based on AFLP marker. J Biot Pert 2003; 8: 60–68.
Sajeeva S, Roya AR, Iangraib B, Pattanayakb B, Dekaa BC. Genetic diversity analysis in the traditional and improved ginger (Zingiber officinale Rosc.) clones cultivated in North-East India. Scientia Horticulturae 2011; 128: 182–188.
Kavitha PG, Kiran AG, Raj RD, Sabu M, Thomas G. Amplified fragment length polymorphism analyses unravel a striking difference in the intraspecific genetic diversity of four species of genus Zingiber Boehm. from the Western Ghats, south India. Cur Sci 2010; 98: 242–247.
Blanco EZ. Genetic diversity of ginger (Zingiber officinale Roscoe.) At the molecular level progress of the last decade 2015; 11(2):190-199.
Sanatombi K, Sanatombi R.Genetic diversity and antioxidant potential of Zingiber montanum (koenig) link ex a diet.Int J Plant Anim Environ Sci 2016; 6:2.
Pandrotra P, Gupta PA, Khan S, Gandhi R, Gupta A. Comparative assessment of ISSR, RAPD, IRAP, & REMAP molecular markers in Zingiber officinale germplasm characterization.In: Sci Hort 2015;194:201-207.
Kar D, Pattanaik PK, Laxmikanta A, Kumar PM, Kamalakanta S, Ananya K, Mishra B. Assessment of genetic diversity among some elite cultivars of ginger (Zingiber officinale Rosc.) using isozyme and protein markers. In: Brazilian Journal of Botany 2014; 37(4):469479.
Mehdi JH, Retno A, Ishak A. Determination of phylogenetic and molecular characteristics of three Malaysian Ginger Cultivars (Zingiber officinale Roscoe) using microsatellite DNA. In: Tropical Life Sciences Research 2013; 24(2):65-76.
Pandrotra P, Kashif HM, Gandhi R, Suphla G, Prakash AG. Retrotransposon based genetic status of North West Himalayan Zingiber officinale revealed high heterogeneity.J Plant Biochem Biotech 2014; 23 (2):211-216.
Singh DH, Devala DK, Singh SB, Singh CB, Devi HS. Assessment of Genetic Stability in Traditional Ginger Cultivated in Manipur, India Based on Molecular and Chemical Markers. Anal Lett 2013; 46(18): 2941-2953.