IN VITRO ANTIDIABETIC, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF CLITORIA TERNATEA L
Keywords:
Clitoria ternaea L, Antioxidant, Phytochemicals, Antidiabetic, Anti-inflammatoryAbstract
Objective: To investigate the in vitro Antidiabetic, Antioxidant and Anti-inflammatory activity of Clitoria ternatea L. leaves and flowers.
Methods: Phytochemicals of leaves and flowers were analysed by using standard methods. In vitro antioxidant studies were carried out for the ethanolic extract of the Clitoria ternatea leaves and flowers using various free radical models such a DPPH, Reducing power assay and Hydrogen peroxide scavenging assay. In vitro antidiabetic assay such as Non-enzymatic glycosylation of haemoglobin assay, Glucose uptake in Yeast cells and Inhibition of salivary-amylase enzyme were carried in ethanolic extract.. In vitro anti-inflammatory activity such as inhibition of albumin denaturation and membrane stabilization assay were performed in ethanolic extract.
Results: Preliminary phytochemical screening of ethanolic extract of the Clitoria ternatea revealed the presence of various bioactive components like alkaloids, flavonoids, steroids, glycosides, phenol, saponin, terpenoids and tannin in both leaves and flowers. Anthroquinone is absent in both the parts studied. The in vitro antidiabetic potential of plant extract was confirmed through non enzymatic glycation, glucose uptake by yeast cells and amylase inhibition methods. Antiinflammatory activity of Clitoria ternaea was also confirmed.
Conclusion: The result of the present study concluded that the ethanolic extracts of Clitoria ternaea L. leaves and flowers possess significant antidiabetic, antioxidant and anti-inflammatory activity. The potential pharmacological activity of Clitoria ternaea L. leaves and flowers might be due to the presence of phytochemicals.
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Meenakshi P, Bhuvaneshwari R, Rathi MA, Thirumoorthi L, Guravaiah DC, Jiji MJ, et al. Antidiabetic activity of ethanolic extract of Zaleya decandra in alloxan-induced diabetic rats. J Appl Biochem Biotechnol 2010;162(4):1153-9.
Balaraman AK, Singh J, Dash S, Maity TK. Antihyperglycemic and hypolipidemic effects of Melothria maderaspatana and Coccinia indica in Streptozotocin induced diabetes in rats. J Saudi pharm SPJ 2010;18(3):173-8.
Kirti S. Prabhu Richard Lobo, Annie Shirwaikar. Antidiabetic properties of the alcoholic extract of Sphaeranthus indicus in streptozotocinnicotinamide diabetic rats. J of Pharmacy and Pharmacology 2008;60:909-16.
Day C. Traditional plant treatments for diabetes mellitus:pharmaceutical foods. The British J of Nutrition 1998;80(1):5-6.
Deka Manalisha, Kalita Jogen Chandra. Preliminary phytochemical analysis and acute oral toxicit study of Clitoria ternatea. Int Res J of pharmacy 2011;2(12):139-40.
Gopumadhavan S, Muralidhar TS, Anturlikar SD, Sujatha MB. Mallika jainu SK, Effect of herbomineral preparation D-400 in Streptozotocin induced diabetic rats. J of Ethanopharmacology 2006;4:5441-46.
Kokate KR, Lakshmiah M. Ramasastri. Chemical component of plants. J of Nutrition and Dietetics 200. 1995;6.
Megha G, Chaudhari Bhoomi B, Joshi Kinnari N, Mistry Brijesh Dabhi, Sanjay lal. In Vitro Antimicrobial Activity of Stem Bark of Bauhinia Purpurea. J Int Sci Press (India) (2013). 4:29-35.
Gupta daksha, kondongala subraya, chandrashekher, Girish pal. In vitro antidiabetic activity of pentacyclic tritrprnoida and fatty acid ester from Bauhinia purpurea. Int J of Pharmacology and Pharm Technology 2013;2:2277-3436.
Rajesh P. Dinesh Kumar Damiki Laloo, Siva Hemalatha. Evaluation of phytochemical and antioxidant activities of the different fractions of Hybanthus enneaspermus Linn F Muell Violaceae. Asian Pacific J of Tropical Medicine 2009:391-6.
Malick CP, Singh MB, Kalyani H, Mensor LI, Menezes FS, Leitao GG, et al. Plant Enzymology and New Delhi;278. 12.
Dos Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method2001. 127-30 p.
Meir S, Kanner J, Akiri B, Hadar SP, J. Determination and involvement of aqueous reducing compounds in oxidative systems of various senescing leaves. J Food Chem 1995;43:1813-7.
Oktay M, Guloin I, Kufrevioglu OI. Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. J LWT Food Science and Technology 2003;36:263-71.
Shinde UA, Phadke AS, Nari AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilization activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. J Fitoterapia 1999;70:251-257.
Okwu DE. Phytochemical and vitamin content of indigenous spices of south eastern Nigeria. J Sustain Adric Environ 2004;6(1):30-7.
Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. J BMC Complement Altern Med 2006;6:35.
Del-Rio ABG, Obdululio J, Casfillo FG, Masin A. Ortuno Uses and properties of citrus flavonoids. J Agric Food Chem 1997;45:4505-15.
Trease GE, Evans WC. Phenols and Phenolic glycosides 1989. 343-83p.
Agoha RC. Medicinal plant of Nigeria. J of Netherland medicinal plants 1974;12:33-4.
Edeoga HOG, Omusun LC. And Uche Chemical composition of Hyptis suavelens and Ocimum gratissimum hybrids from Nigeria. African J of Biotechnology. 2006;5(10):892-5.
Karou DA, Savadogo A, Canini S, Yameogo C. Montisano Antibactrial activity of alkaloids from sida acuta. Afr J Biotechnol 2003;4:1452-7.
Clifford J. Bailey Caroline Day. Review article Traditional Plant Medicines as Treatments for Diabetes Diabetes care. 1989;12(8):24-8.
Raghavendra NM, Reddy NV, Sneha J. Suvarchala and Anarthe. In Vitro Antioxidant and Antidiabetic activity of Asystasia gangetica Chinese Violet Linn Acanthaceae. Int J of Res in Pharm and Biom Sci 2010;1(2):2229-3701.
Shreedhara CS, Vaidya VP, Vagdevi HM, Latha KP, Muralikrishna KS, Krupanidhi AM. Screening of Bauhinia purpurea Linn. for analgesic and anti-inflammatory activities. Indian J Pharmacol 2009;41(2):75-9.
Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. J Free Radic Biol Med 1996;21(6):895-902.
Velavan S, Nagulendran K, Mahesh R. In vitro antioxidant activity of Asparagus racemosus root. J Pharmacog Magaz 2007;26-33.
Shinde UA, Phadke AS, Nari AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilization activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. J Fitoterapia 1999;70:251-7.