IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITY OF CARDIOSPERMUM HALICACABUM L. AGAINST EAC CELL LINE
Keywords:
Cardiospermum halicacabum L, Ehrlich Ascites Carcinoma, Free radicals, AntioxidantAbstract
Objective: To investigate the in vitro antioxidant and anticancer activity of chloroform and ethanol extracts of Cardiospermum halicacabum L. leaves.
Methods: Phytochemicals were analysed by using standard methods. In vitro antioxidant studies were carried out for the chloroform and ethanol extracts of the Cardiospermum halicacabum using various free radical models such a DPPH, Reducing power assay, nitric oxide scavenging, hydrogen peroxide (H2O2) scavenging, super oxide scavenging activity and ABTS. In vitro cytotoxic assay such as trypan blue dye exclusion and MTT assays were carried out against EAC cell line.
Results: Preliminary phytochemical analysis of the Cardiospermum halicacabum L. was carried out and it revealed the presence of alkaloids, coumarine, flavones, saponins, steroids, sugar, tannins and terpenoids. The results revealed that the chloroform extract has significant antioxidant potential than ethanol extract. The result revealed that the chloroform extracts of Cardiospermum halicacabum L. showed pronounced anticancer activity against Ehrlich Ascites Carcinoma (EAC) cell line than ethanol extract.
Conclusion: The result of the present study concluded that the chloroform extract of Cardiospermum halicacabum L have significant antioxidant and anticancer activity then the ethanolic extract. The potential antioxidant and anticancer activity of Cardiospermum halicacabum L might be due to the presence of phytochemicals.
Downloads
References
Siegel AB and Zhu AX. Metabolic syndrome and hepatocellular carcinoma:two growing epidemics with a potential link. J Cancer 2009;115:5651-61.
Kushi LH, Doyle C and Mc Cullough M. American Cancer Society Guidelines on nutrition and physical activity for cancer prevention:reducing the risk of cancer with healthy food choices and physical activity. CA Cancer J Clin 2012;62 (1):30-67.
Kinzler, Kenneth Vogelstein and Bert. The genetic basis of human cancer. J New York:Mc Graw-Hill, Medical Pub 2002;005-009.
Kuper H, Boffetta P, Adami HO. Tobacco use and cancer causation:association by tumour type. J of Internal Medicine 2002;252 (3):206-24.
Ellestad GA. Structural and conformational features relevant to the anti-tumor activity of caliche amiciny. J Chirality 2011;23, 660-71.
Benedek B, Ziegler A, Ottersbach P. Absence of mutagenic effects of a particular Symphytum officinale L. liquid extract in the bacterial reverse mutation assay. Phytotherapy Res 2010;24:466-8.
Jeyadevi R, Sivasudha T, Rameshkumar A, James Harnly and Long-Ze Lin. Phenolic profiling by UPLC–MS/MS and hepatoprotective activity of Cardiospermum halicacabum against CCl4 induced liver injury in Wistar rats. J of Functional Foods 2013;289-98.
Venkatesh Babu KC, Krishnakumari S. Cardiospermum halicacabum suppresses the production of TNF-alpha and Nitric oxide by Human Peripheral Blood Mononuclear cells. African J of Biomedical Res 2006;9:95-99.
Abdul Waheed, Yamin Bibi, Sobia Nisa, Fayyaz Chaudhary, Sumaira Sahreen and Muhammad Zia. Inhibition of human breast and colorectal cancer cells by Viburnum foetens L. extracts in vitro. Asian Pacific J of Tropical Disease 2013;3(1):32-6.
Girish H, Sudarshana MS, Rati Rao E. In vitro Evaluation of the Efficacy of Leaf and its Callus Extracts of Cardiospermum halicacabum L. On Important Human Pathogenic Bacteria. J Advances in Biological Res 2008;2 (1-2):34-8.
Brindha P, Sasikala, Bhima Rao. Pharmacognostic studies on Coleus Aromaticus Benth. J Indian Borage 1981;12:17-31.
Gyamfi MA, Rati Rao E, Aniya Y. Antioxidant properties of thonningianin A, isolated from the African medicinal herb. Thonningia sanguine. J Biochem Pharmacol 2002;63:1725-37.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr 1986;44:307-15.
Ruch RJ, Chung SU, Klaunig J E. Spin trapping of superoxide and hydroxyl radicals. J Methods in Enzymology 1984;105:198-209.
Nathan CF, Rati Rao P, Hibbs JJB. Role of nitric oxide synthesis in macrophage antimicrobial activity. J Curr Opin Immunol 1991;3:65-70.
Nishimiki M, Rao N A, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. J Biochem Biophys Res Commun 1972;46:849-53.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. J Free Radical Biology and Medicine 1999;26:231–1237.
Fangli Hou, Murray C J and Ezzati M. Hepatoprotective and antioxidant activity of anthocyanins in black rice bran on carbon tetrachloride-induced liver injury in mice. J of Functional Foods 2013;1705-13.
Gothoskar S V and Ranadive K J. Anticancer screening of SAN-AB, An extract of marking nut Semicarpus anacardium. Indian J Exp Biol 1971;9:372-5.
Sheeja K R, Kuttan G and Kuttan R. Cytotoxic, antitumour activity of Berberin. J Amala Res. Bull 1997;17:73-6.
Scudiero DA, Shoemaker RH and Paul KD. Evaluation of soluble terazolium formazan assay for cell growth and drug sensitivity in clusters using human and other tumor cell lines. J Cancer Res 1988;48:4827-33.
Nagendra Prasad, Shahira Ezzat and Laila Ismail. Antioxidant and anticancer activities of high pressure-assisted extract of longan (Dimocarpus longan Lour.) fruit pericarp. J Innovative Food Sci and Emerging Technologies 2009;10:413-9.
Raju Senthil Kumar, Balasubramanian Rajkapoor and Perumal Perumal. In vitro and in vivo anticancer activity of Indigo feracassioides Rottl. Ex. DC. Asian Pacific J of Tropical Medicine 2011;379-85.
Rajesh Kumar G, Murugananthan K, Nandakumar K, Sahil Talwar. Isolation of anxiolytic principle from ethanolic root extract of Cardiospermum halicacabumâ€. J Phytomedicine 2011;219-23.
Mohammad Sekendar Ali, Mohammad Ruhul Amin, Chowdhury Mohammad Imtiaz Kamal, Mohammad Aslam Hossain. In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pacific J of Tropical Biomedicine 2013;3(6):464-9.
Nesrine Ouda Amari, Mohamed Bouzouina, Abdellah Berkani, Brahim Lotmani. Phytochemical screening and antioxidant capacity of the aerial parts of Thymelaea hirsute L. Asian Pacific J of Tropical Disease 2014;4(2):104-9.
Pradeep Pratap Singh, Ambika and Chauhan S M S. Activity guided isolation of antioxidants from the leaves of Ricinus communis L. J Food Chemistry 2009;1069-72.
Velavan Sivanandham. Free Radicals In Health And Diseases-A Mini Review. J Pharmacologyonline Newsletter 2011;1062-77.
Nabila Benariba, Murray CJ. and Ezzati M. Phytochemical screening and free radical scavenging activity of Citrullus colocynthis seeds extracts. Asian Pacific J of Tropical Biomedicine 2013;3(1):35-40.
Shahidi F and Zhong Y. Novel antioxidants in food quality preservation and health Promotion. European J of Lipid Sci and Technology 2010;112, 930-40.
Rabia Naz and Asghari Bano. Phytochemical screening, antioxidants and antimicrobial potential of Lantana camara in different solvents. Asian Pacific J of Tropical Disease 2013;3(6):480-6.
Miek Jonga, Ulrike Ermuth and Matthias Augustin. Plant-based ointments versus usual care inthe management of chronic skin diseases:a comparative analysis on outcome and safety. J Complementary Therapies in Medicine 2013;453-9.
Hussein Farhan, Murray CJ, Ezzati M. Phytochemical screening and antioxidant activity of Lebanese Eryngium creticum L. Asian Pacific J of Tropical Biomedicine 2012;S1217-S20.
Constantin T, Konstantinos Dimas, Zacharias Sofianos. Metabolism and Anticancer Activity of the Curcumin Analogue, Dimethoxycurcumin. J Clinical Cancer Res 2007;13:1269-77.
Isha Dhamija, Nitesh Kumar, Manjula S N., Vipan Parihar, Manjunath Setty M, Pai R. Preliminary evaluation of in vitro cytotoxicity and in vivo antitumor activity of Premna herbacea Roxb. in Ehrlich ascites carcinoma model and Dalton’s lymphoma ascites model. J Experimental and Toxicologic Pathology 2013;235-42.
Ursula Sauer, Shahira Ezzat, Laila Ismail. Applicability of rat precision-cut lung slices in evaluating nanomaterial cytotoxicity, apoptosis, oxidative stress, and inflammation. J Toxicology and Applied Pharmacology 2014;1–20.
Eduardo Parisotto, Murray CJ, Ezzati M. The antitumor activity of extracts from Cordia verbenacea D.C. obtained by supercritical fluid extraction. The J of Supercritical Fluids 2012;101-7.