STATISTICAL ANALYSIS OF THE ANTIMICROBIAL ACTIVITY OF WRIGHTIA TINCTORIA LEAF AND BARK EXTRACTS
Keywords:
Wrightia tinctoria, Antibacterial activity, Statistical analysis, Standard antibiotics, Drug formulationAbstract
Objective: Wrightia tinctoria has been extensively used in Folk medicine. It has been reported to have good analgesic, anti-inflammatory, anthelmintic, antiulcer, antidysentric, antidiabetic, anticancer, antipyretic activities and also effective in the treatment of psoriasis. The present investigation was undertaken to statistically evaluate the antimicrobial activity of the leaf and bark extracts of Wrightia tinctoria.
Methods: The antibacterial and antifungal activities of leaf coconut oil extracts (1, 4, 7 and 15 days exposure to sunlight), bark and leaf methanol, ethyl acetate, chloroform and petroleum ether extracts of Wrightia tinctoria (Apocynaceae) against nine pathogenic bacteria such as Bacillus cereus, Enterobacter faecalis, Salmonella paratyphi, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Pseudomonas aeruginosa and Serratia marcescens and two fungi viz. Aspergillus niger and Penicillium chrysogenum were statistically analyzed so as to evaluate the medicinal potential of these plant extracts.
Results: Wrightia tinctoria leaf and bark methanol extracts were found to be potent extracts and their activity is quite comparable with the standard antibiotics such as tobramycin and gentamycin sulphate screened under similar conditions.
Conclusion: Methanol extracts of the leaves and bark of Wrightia tinctoria can be used as a potential external antiseptic and can be incorporated into drug formulations.
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References
Kirtikar KR, Basu BD. J Indian Medicinal Plants 1981;2187-8.
George V, Koshy AS, Pushpangandan P. Tryptanthrin from Wrightia tinctoria. J Fitoterapia 1996;67(6):553-5.
Muruganandam AV, Bhattacharya SK, Ghosa S. Indole and flavonoid constituents of Wrightia tinctoria, W. tomentosa and W. coccinea. Indian Journal of Chemistry B 2000;39(2):125-31.
Vijayan P, Raghu C, Ashok G, Dhanaraj SA, Suresh B. Antiviral activity of medicinal plants of Nilgiris. Indian J of medical Res 2004;120(1):24-9.
Kenneth D, Antiviral T, Dragar C. of Undaria Pinnatifida against Herpes Simplex Virus. J Phytotheraphy Res 2004;18:551-3.
Chopra RN, Nayar SI, Chopra IC, Plants C. Glossary of Indian Medicinal New Delhi, India:;p. 259. BMC Complement Altern Med. 1956.
Joshi SG. Medicinal plants, Oxford and IBH publishing Co 2000.
Khare CP, New H. Indian medicinal plants, Springer-Verlag Berlin. J BMC Complement Altern Med 2007:721-2.
Joshi MC, Patel MB, Mehta PJ. Some folk medicines of drugs. J Bull of Medico Ethnobotanical Res 1980;1:8-24.
Shah GL, Gopal GV. Ethnomedical notes from the tribal inhabitants of the north Gujarat (India) Journal of Economic & Taxonomic Botany. J BMC Complement Altern Med 1988;6:193-221.
Reddy RM, Changamma C, Reddanna P, Govindappa S. In vitro effects of gossypol and lactic acid on rat uterus and ovary during implantation and antiimplantation. Indian J Exp Biol 1989;27(12):1017-9.
Reddy MB, Reddy KR, Reddy MN. A survey of plant crude drugs of Ananthapur District, Andhra Pradesh, India. Inte J of Crude Drug Res 1989;27:145–55.
Siddiqui MB, Hussain W. Traditional antidotes of snake poison. J Fitoterapia 1990;61:41-4.
Omar S, Lemonnier B, Jones N, Ficker C, Smith ML, Neema C, et al. Antimicrobial activity of extracts of eastern North American hardwood trees and relation to traditional medicine. J Ethnopharmacol. 2000;73(1-2):161-70.
Daniel M, Sabnis SD. Chemotaxonomical studies on apocynaceae. Indian J Exp Biol 1978;16(4):512-3.
Joji LR, Beena J, Anjana JC, Ruveena TN. Evaluation of antibacterial activity of Trichosanthes cucumerina L. and Cassisa didymobotrya Fres leaves Int J of Pharmacy and Pharm Sci 2010;2(4):153-5.
Joji LR, Spandana G, Reshmadevi J. Beena Jose, Evaluation of antibacterial and antioxidant activities of the leaf and bark extracts of Azadtrachta indica a. Juss World J of Pharm Res 2012;1(3):1-12.
Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. J BMC Complement Altern Med 2006;6:35.