COMPARATIVE STUDY OF ANTIOXIDANT AND ANTICANCER ACTIVITY OF ALPINIA CALCARATA AND ALPINIA GALANGA
DOI:
https://doi.org/10.22159/ijpps.2017v9i12.21599Keywords:
Alpinia galanga, Alpinia calcarata, Antioxidant activity and anticancer activityAbstract
Objective: The present study was to determine in vitro antioxidant and anticancer activity of Alpinia calcarata and Alpinia galanga.
Methods: The phytochemical screening of rhizome of aqueous extract of Alpinia calcarata and Alpinia galanga was performed using standard procedures. The total phenolic and flavanoid content were determined by Folin-Ciocalteau and Aluminium chloride reagents. The various antioxidant assays and cytotoxic assays (MTT) for Alpinia calcarata and Alpinia galanga was performed using standard methods (DPPH radical scavenging assay, Nitric oxide radical scavenging assay, Reducing power assay, Phosphomolybdenum reduction assay)
Results: The preliminary phytochemical screening of Alpinia calcarata and Alpinia galanga showed the presence of flavanoids, phenols, terpenoids, carbohydrates and proteins. The phenolic content of aqueous extracts of rhizomes of Alpinia calcarata was 454.05 μg/mg and Alpinia galanga was 480.13 μg/mg and were expressed as gallic acid equivalent. The flavanoid content of aqueous extracts of rhizomes of Alpinia calcarata was 36.34 μg/mg and Alpinia galanga was 67.68 μg/mg and were expressed as quercetin equivalent. In DPPH assay, Alpinia galanga showed 95.36% where as Alpinia calcarata showed 54.54% at 120 μg/ml. The maximum NO• radical scavenging activity was 59.44% for Alpinia calcarata and was 73.10% for Alpinia galanga at 120 µg/ml concentration. The maximum reducing property was found at the 120 μg/ml of aqueous extract of rhizomes of Alpinia galanga which was higher than the Alpinia calcarata. In Phosphomolybdenum assay, the aqueous extracts of rhizomes of Alpinia calcarata and Alpinia galanga were 55.47% and 78.38% respectively. The results of present investigation indicated that rhizome of aqueous extract of Alpinia galanga showed the highest antioxidant activity in all the assays than Alpinia galanga. The cytotoxicity assay results indicated that rhizome of aqueous extract of Alpinia galanga showed 88.36% cell viability whereas Alpinia calcarata showed 73.59% cell viability.
Conclusion: The results obtained in the present study indicate that rhizome of Alpinia galanga are abundant in phenols and flavanoids which may be useful for the development of anticancer drug.
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Sharma SC, Goel AK. The need for ex-situ conservation of endangered plants from tropical India. Botanic Gardens Conservation News 1990;1:16-9.
Narula A, Srivastava PS, Rangaswamy NS. In vitro cultures studies on Dioscorea species. J Tro Med Plants 2000;1:60-74.
Owolabi J, Omogbai EKI, Obasuyi O. Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigelia africana (Bignoniaceae) stem bark. Afr J Biotech 2007;6:882-5.
Anonymous. Traditional medicine Strategy Report, World Health Organization, Rome; 1992.
Robbers JE, Tyler VE. Herbs of choice-the therapeutic use of phytomedicines. Int J Phrm Sci 2002;3:199-203.
Arambewela LSR, Arambewela LDAM, Ratnasooriya WD. Antinociceptive activities of aqueous and ethanolic extracts of Alpinia calcarata rhizomes in rats. J Ethanopharmacol 2004;95:311-6.
Arambewela LSR, Arambewela LDAM. Antioxidant activities of Ethanolic and hot aqueous extracts of Alpinia calcarata rhizomes. Aust J Med Herbalism 2005;17:91-4.
Ratnasooriya WD, Jayakody JR. Effects of aqueous extract of Alpinia calcarata rhizomes on the reproductive competence of male rats. Acta Biol Hung 2006;57:23-35.
Arambewela LSR, Arambewela LDAM, Ratnasooriya WD. Effect of Alpinia calcarata rhizomes on ethanol-induced gastric ulcers in rats. Phcog Mag 2009;4:226-31.
Leal-Cardoso JH. Effects of essential oil of Alpinia Zerumbet on the compound action potential of the rat sciatic nerve. Phytomed 2004;11:549-53.
Bezerra MA. Myorelant and antispasmodic effects of the essential oil of Alpinia speciosa rat ileum. Phytother Res 2000;14:549-51.
Lahlou S, Interaminense LF, Leal-Cardoso JH, Duarte GP. Antihypertensive effects of the essential oil of Alpinia Zerumbet and its main constituent, terpinen-4-ol, in DOCA-salt hypertensive conscio rats. Fundam Clin Pharmacol 2003;17:323-30.
De Moura RS. Antihypertensive and endothelium-dependent vasodilator effects of Alpinia Zerumbet, a medicinal plant. J Cardiovasc Pharmacol 2005;46:288-94.
Fuloria NK, Fuloria S. Isolation of β-sitosterol diarabinoside from rhizomes of Alpinia galanga. World Acad Sci Eng Technol 2012;72:1455-7.
Jaju SB, Indurwade NH, Sakarkar DM, Fuloria NK, Ali MD, Basu SP. Isolation of β-sitosterol diglucosyl caprate from Alpinia galanga. Pharma Res 2010;2:264-6.
Jaju SB, Indurwade NH, Sakarkar DM, Fuloria NK, Ali MD, Das S, et al. Galangoflavonoid isolated from rhizome of Alpinia galanga (L) Sw (Zingiberaceae). Trop J Pharm Res 2009;8:545-50.
Seo J, Cho S, Park S, Lee E, Lee J, Han S, et al. 1_-Acetoxychavicol acetate isolated from rhizomes Alpinia galanga ameliorates ovalbumin-induced asthma in mice. Plos One 2013;8:e56447.
Reddy JL, Jose B, Ruveena TN. Evaluation of antibacterial activity of the seed essential oil and leaf extracts of Alpinia galanga (L.) Willd. Asian J Biochem Pharma Res 2011;3:270–6.
Deepa S, Prabakaran G. Wound healing activity of ethanolic extract of Alpinia galanga leaves. J Bio Innovation 2012;1:120-6.
Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis chapman and hall. London, Ltd; 1973. p. 149-88.
Trease GE, Evans IC. Textbook of Pharmacognosy. 12th edn. Balliere Tindall, London; 1983.
Bao JS, Cai YZ, Sun MG, Wang, Corke H. Anthocyanins, flavonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. Agric Food Chem 2005;53:2327-32.
Sultana B, Anwar F, Przybylski R. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia Arjuna, Acacia nilotica, and Eugenia jambolana Lam Trees. Food Chem 2007;104:1106-14.
Akowuah GA, Ismail Z, Norhayati I, Sadikun A. The effects of different extraction solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the free radical-scavenging activity. Food Chem 2005;93:311-7.
Athukorala Y, Kim KN, Jeon YJ. Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 2006;44:1065-74.
Olabinri BM, Odedire OO, Olaleye MT, Adekunle AS, Ehigie LO, Olabinri PF. In vitro evaluation of hydroxyl and nitric oxide radical scavenging activities of artemether. Res J Biol Sci 2010;5:102-5.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
Mosmann T. Rapid colourimetric assay for cellular growth and survival: application to proliferation and cytotoxicity tests. J Immunol Methods 1983;65:55-63.
Bougard F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant Sci 2001;161:839-51.
Walton NJ, Brown DE. Chemicals from plants: perspectives on plant secondary products; Imperial College Press: London; 1999.
Swapana N, Jotinkumar T, Devi CB, Singh MS, Singh SB. Total phenolic, total flavonoid contents and antioxidant activity of a few indigenous, Fruits Grown in Manipur. Bio-Scan 2012;7:73-6.
Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biol Med 1996;20:933-56
Ramassamy C. Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: a review of their intracellular targets. Eur J Pharmacol 2006;545:51-64.
Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical scavenging activity of foods by using 2, 2-diphenyl-1-picrylhydrazyl. Biosci Biotechnol Biochem 2002;62:1201-4.
Wong LF, Lim YY, Omar M. Antioxidant and antimicrobial activities of some Alpinia species. J Food Biochem 2009;33:835-51.
Lata H, Ahuja GK. Role of free radicals in health and disease. Indian J Physiol Allied Sci 2003;57:124â€8.
Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143-52.
Majidul I, Rumana Y, Hanif A, Polash CK, Ayshasiddeka FI, Rowshanul H, et al. Antioxidant activity of ethanolic extract of Alpinia calcarata Rosc. Rhizome J Pharma Phytochem 2017;6:469-74.
Alasalvar C, Karamac M, Amarowicz R, Shahidi F. Antioxidant and antiradical activities in extracts of hazelnut kernel (Corylusa vellana L.) and hazelnut green leafy cover. J Agric Food Chem 2006;54:4826-32.
Ak T, Gulcin I. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 2008;174:27–7.
Arunkumar K, Chandrashekar KR. Phytochemical evaluation and in vitro antimicrobial and antioxidant studies of leaf and stem bark extracts of Polyalthia fragans (Dalz) bedd-an endemic species of Western Ghats. Int J Pharma Pharm Sci 2017;9:20–4.
Duh PD. Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free radical and active oxygen. J Am Oil Chem Soc 1998;75:455-61.
Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
Loo AY, Jain K, Darah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem 2008;107:1151-60.
Enock KO, Palanirajan A, Deivasigamani M, Chinthamony A, Velliyur KG. Antioxidant, cytotoxic and apoptotic activities of crude extract of Alpinia purpurata on cervical cancer cell line. Int J Pharm Sci Rev Res 2016;36:28-34.
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