• Khumanthem Deepak Singh Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, INDIA
  • Dipak Chetia Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, INDIA
  • Biplab D. E. Regional Institute of Pharmaceutical Science & Technology, Agartala, Tripura, INDIA



Allium hookeri, Antiulcer, Isolated flavonoid, In vivo antioxidant


Objective: The main objective of this study was to investigate the antiulcer potential of an isolated flavonoid compound from Allium hookeri (AH).

Methods: Oral administration of ethanol-induced ulcer to the mucosal layer of the stomach in the rats. The ulcer score and percentage protection was calculated from the stomach and gastric mucosal scrapping was carried out for the biochemical studies. Antioxidant study was carried out in liver and histopathological study of the ulcer stomach was performed.

Results: Phytochemical investigation of methanolic extract of AH (MEAH) leaves afforded a new flavonoid compound characterized as 3, 5, 6-trihydroxy-2-(4-hydroxy-3-(6-hydroxy-4, 5, 6, 7-tetrahydro benzofuran-7-yl) phenyl-7-methoxy-4H-chromen-4-one (Compound 1). The flavonoid compound at the dose of 5 mg/kg showed a significant change in ulcer index by reducing the ulcer index and increased in percentage protection. It also increased the in vivo antioxidant activity by increasing the superoxide dismutase (SOD), glutathione (GLU) and catalase (CAT) levels. The isolated compound reversed the increase of mucin content in the mucosal scrapings. The histopathology study showed the reversed of normal gland texture in the mucous layer of the standard and compound 1 treated stomach when compared to the negative control.

Conclusion: Present findings demonstrated the significant antiulcer activity and antioxidant activity of the isolated flavonoid compound as the supporting factor.


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Tripathi KD. Essentials of medical pharmacology. 6th ed. Jaypee Brothers, New Delhi; 2008.

Berardi RR, Welage S. Peptic ulcer disease. In: Dipiro TJ, RL Talbert, G Yes, G Matzke, G Wells, M Posey. Editors. Pharmacotherapy: A Pathophysiologic Approach. 6th ed. McGraw-Hill; 2005.

Suerbaum S, Michetti S. Helicobacter pylori infection. N Engl J Med 2002;347:1175-86.

Clouatre D, Rosenbaum M. The diet and benefits of HCA. Keats Publishing, New York; 1994.

Kalaichelvi K, Sharmila S, Dhivya SM. In vitro antioxidant and antiulcerogenic activity of Cayratia pedata var. glabra against experimentally induced gastric lesions in wistar strain albino rats. Asian J Pharm Clin Res 2018;11:105-10.

Ayam VS. Allium hookeri Thw. Enum. A lesser-known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur. Afr J Food Agric Nutr Dev 2011;11:5389-412.

Sukumar S. The economic plants of Manipur and their uses. Career publications, Imphal; 2009.

Singh KB, Singh NM. Antioxidant and free radical scavenging potential of Allium hookeri thwaites roots extract studied using in vitro models. J Adv Biol 2014;4:275-85.

Singh KD, Chetia D, Junejo JA. Antiulcer and in vitro antioxidant activity of Allium hookerii: an ethnomedicinal plant of Manipur. Asian J Pharma Clin Res 2015;8:137-42.

Singh KD, Chetia D, Mazumdar S. Antidiabetic activity of methanolic extract of Allium hookerii leaves. Indo Am J Pharm Res 2013;3:4098-104. [Last accessed on 10 Nov 2017]

Hollander D, Tarnawski A, Krause WJ, Gergely H. Protective effect of sucralfate against alcohol-induced gastric mucosal injury in the rat. Gastroenterology 1985;88:366-74.

Winzler RJ. Determination of serum glycoproteins. Methods Biochem Anal 1955;2:279-311.

Dische Z, Borenfreund E. A spectrophotometric method for the micro determination of hexosamines. J Biochem 1950;184:517-22.

Dische Z, Schettles LB. A specific colour reaction for methyl pentoses and spectrophotometric micro method for determination. J Biochem 1948;175:595-603.

Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959;234:1971-5.

Lowry OH, Rosenborough NI, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951;193:265-75.

Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr 1986;44:307-15.

Sadhu SK, Okuyama E, Fujimoto H, Ishibashi M. Separation of Leucas aspera, a medicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities. Chem Pharm Bull 2003;51:595-8.

Luck H. Catalase. In: Methods of enzymatic analysis. Edited by Bergmeyar HU, Verlag Chemie. Academic Press: New York; 1963.

Kehar U, Wahi PN. Cytologic and histologic behaviour patterns of the premalignant lesions of the cervix in experimentally induced cervical dysplasia. Acta Cytologica 1967;11:1-15.

Halliwell B. Use of desferrioxamine as a ‘probe’ for the iron-dependent formation of hydroxyl radicals. Evidence for a direct reaction between desferal and the superoxide radical. Biochem Pharmacol 1985;34:229-33.

Wang SY, Kuo YH, Chang HN, Kang PL, Tsay HS, Lin KF, et al. Profiling and characterization antioxidant activities in Anoectochilus formosanus hayata. J Agric Food Chem 2002;50:1859-65.

Hyland K, Voisin E, Banoun H, Auclair C. Superoxide dismutase assay using alkaline dimethylsulfoxide as superoxide anion-generating system. Anal Biochem 1983;135:280-7.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.

Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical J 2013;21:143-52.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.

Sedlak J, Lindsay RH. Estimation of total, protein-bound and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 1968;25:192-205.

Halliwell B, Guttiredge JM. Free radicals biology and medicine. Oxford: Clarendon Press; 1989.

Piper DW, Stiel DD. Pathogenesis of chronic peptic ulcer, current thinking and clinical implications. Med Prog 1986;2:7-10.

Zubaida Y, Ying W, Elias B. Phytochemistry and pharmacological studies on Solanum torvum Swartz. J Appl Pharm Sci 2013;3:152-60.

Narayan S, Devi RS, Jainu M, Sabitha KE, Devi CSS. Protective effect of the polyherbal drug, ambrex in the ethanol-induced gastric mucosal lesion in experimental rats. Indian J Pharmacol 2004;36:34-7.

Nasuti C, Gabbianelli R, Falcioni G, Cantalamessa F. Antioxidant and gastroprotective activities of anti-inflammatory formulations derived from chestnut honey in rats. Nutr Res 2006;26:130-7.

Rainsford KD. Gastric ulcerogenecity of non-steroidal anti-inflammatory drugs in mice with mucosa sensitized by cholinomimetic treatment. J Pharm Pharmacol 1978;39:669-72.

Eun YS, Chang WP, Go EH, Ki WL, Chi HL. Effect of addition of Allium hookeri on the quality of fermented sausage with meat from sulfur fed pigs during ripening. Korean J Food Sci 2014;34:263-72.

Hee JL, Jae EB, Nami J. Quality characteristics and storage stability of bread with Allium hookeri powder. Korean J Food Nutr 2014;27:318-29.



How to Cite

Singh, K. D., D. Chetia, and B. D. E. “NEW FLAVONOID COMPOUND FROM ALLIUM HOOKERI THWAITES AS A GASTROPROTECTIVE AGENT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 5, May 2018, pp. 24-30, doi:10.22159/ijpps.2018v10i5.24341.



Original Article(s)