NEUROPROTECTIVE POTENTIAL AND EFFICACY OF NEURODEGENERATIVE DISORDERS OF FRUIT EXTRACT OF AEGLE MARMELOS
Keywords:
Hypoxia, Alzheimer, Parkinsonism, Aegle marmelos, NeuroprotectionAbstract
Objective: To evaluate the neuroprotective and neurodegenerative disorder of Aegle marmelos.
Methods: The present study was carried out to evaluate the Alzhiemer and Parkinsonism disorders by Actophotometer test. Water maze test and Reserpine induced Parkinsonism models in sodium nitrite induced hypoxic rats.
Results: The Aegle marmelos fruit extracts improved the cognitive defects in hypoxic rats. The inquiry into behavioral studies indicates that sodium nitrite induced neurotoxicity, decreases the locomotor activity and decreases the habituation memory. The study shows a significant reduction (p<0.01) in both doses (200mg/kg and 400mg/kg) of Aegle marmelos extract in water maze test and locomotor activity test. Also the experiment showed the significant reduction in muscular rigidity by decreasing the gripping latency on the rod. The Aegle marmelos fruit extract produced a reduction in the Acetylcholin esterases (AChE) and glutamate levels but significantly increased in dopamine level. Increasing in AChE activity indicates that it ameliorates cholinergic function by inhibiting sodium nitrite which induces increase in AChE activity.
Conclusion: This study strongly suggests that the Aegle marmelos fruit extract attributed its prominent Alzheimer and Parkinsonism activity.
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References
Antonio R Damasio, Joao Lobo. Antunes psychiatric effects in Parkinsonism diseases. J Neurolol Neurosurgery Psychiatry 1971;34:502-7.
Beckman JS. The double-edged role of nitric oxide in brain function and superoxide anion. J Dev Physiol 1991;15:53-9.
Cazevieille C, Muler A, Meyner F, Bonne C. Superoxide and nitricoxide cooperation in hypoxia/reoxygenation induced neuron injury. Free Rad Biol Med 1993;14:389-95.
Coyle JT, Prince DL, Delong MR. Alzheimers disease. A disorder of cortical cholinergic innervations. Sci 1983;219:1184-90.
Dawson VL, Dawson TM, London ED, Bredt DS, Synder SH. Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures. Proc Natl Acad Sci of USA 1991;88:6368-71.
Vincent SR. Nitric oxide a radical neurotransmitter in the central nervous system. Neurobiol 1994;42;129-60.
Kresmir K. Early effect of hypoxia on brain cell function, Brain Res 1998;219:2-4.
Sharmila U, Kshama K, Shanbhag, Suneetha G, Balachandra, Subramanya U. Hypoglycemic and antioxidant activity of Aegle marmelos. Indian J Physiol Pharmacol 2004;48:476-80.
Trease GE, Evans WC. Pharmacognosy 2002;15:309-15.
Nyakas C, Buwala B, Kraners. Effect of nitrite induced prenatal hypoxia. Neurosci 1994;53:541-59.
Kulkarni SK. Handbook of Experimental Pharmacology 2004;3:117-9,
Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984;11:47-60.
Ellan GL, Courteny KD, Anders V, Featherstone RM. A new and rapid colorimetric determination of acetylcholine esterase activity. Biochem Pharmacol 1961;7:88-95.
Bernt E, Bergmeyer HU. Glutamate UV-assay with glutamate dehydrogenase and NAD. Methods Enzym Anal 1965;2:1704-08.
Schlunff M, Litchtensteiger W, Langemann H, Waser PG. A fluorimetric micromethod for the simultaneous determination of serotonin, noradrenaline and dopamine in milligram amount of brain tissue. Biochem Pharmacol 1974;23:2337-446.
Gupta SK. Antiparkinsonian drugs. Drug Screening Methods; 2004. p. 107.
Vogel GH. Psychotropic and neurotropic activity. Drug Disc Evaluation 2002;2:397-400.
Olesen SP, Moller A, Mordvintcev PI, Busse R, Mulsch A. Regional measurement of NO formed in vivo during brain ischemia. Acta Neurosci 1997;95:214-9.