ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA
Keywords:Commelina nudiflora, Phytochemical compounds, Antioxidants, Methanol extract
Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained fromÂ CommelinaÂ nudifloraÂ were investigated for the free radical scavenging effects and phytochemical analysis.
Methods: The antioxidative effect of the essential oil, methanol extractsÂ andÂ methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and ï¬‚avonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively.Â The phytochemical analyses of the essential oil, methanol extractsÂ andÂ methanol fractions were performed by gas chromatography and mass spectrometry (GCMS).Â
Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetateÂ andÂ chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoidsÂ andÂ tannins etc.Â The identified bioactiveÂ constituents of essential oil, methanol extract and methanol fractions ofÂ C. nudiflora wereÂ indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl,Â phenol, benzyl alcohol, eugenol,Â phenol, 2, 4-bis (1,1-dimethylethyl),Â hexadecanoic acid, ethyl ester (palmitic acid ester),Â n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid)Â andÂ phytol.Â All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.
Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractionsÂ ofÂ C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.
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