• CAYSANDRA AUDREY MICHELLE ELWON Biotechnology Research Institute
  • MUHAMMAD DAWOOD SHAH Biotechnology Research Institute, Borneo Marine Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia
  • MOHAMMAD IQBAL Biotechnology Research Institute




O corymbosa, Alloxan, Hypoglycaemic, Hypolipidemic, Antioxidative, Diabetes mellitus


Objective: The present study was designed to demonstrate the antioxidant, hypolipidemic and hypoglycaemic potentials of aqueous extract of O. corymbosa against alloxan-induced diabetes in rats.

Methods: O. corymbosa extract was tested for phytochemical screening, total phenolic, flavonoids content and DPPH free radical scavenging activity. Diabetes was induced in Sprague Dawley rats by administration of alloxan monohydrate (65 mg/kg b. w i. v). The aqueous extract of O. corymbosa at a dose of 100 mg/kg and 200 mg/kg were administered through gavage feeding daily to diabetic induced rats for 14 d. The effect of aqueous extract of O. corymbosa was assessed on blood glucose, body weight, lipid peroxidation, catalase activity, glutathione and lipid profile. Pancreatic tissues were also examined by haematoxylin and eosin staining methods.

Results: Phytochemical screening shows the presence of tannins, saponins, phlobatannins and flavonoids. Total phenolic content was found to be 22.85±0.21 mg/g, IC50 is 450±1.39 µg/ml and total flavonoids content was found to be 4.25±0.09 mg/g of extract. The results of the present study showed that O. corymbosa can lower blood glucose and lipid parameters except for HDL. The levels of antioxidant enzymes CAT and GSH were increased along with the decreased in LPO level by the pre-treatment of animals with O. corymbosa. Microscopic examination of pancreatic sections revealed that diabetic rats treated with O. corymbosa extracts at either dose have normal architecture structure of islets.

Conclusion: These results indicate that O. corymbosa may be effective as a hypoglycaemic and antihyperlipidemic agent.


Download data is not yet available.


Diabetes in Malaysia 2014. International Diabetes Federation (IDF); 2015.

Gupta PD, De A. Diabetes mellitus and its herbal treatment. Int J Res Pharm Biomed Sci 2012;3:706-21.

Yadav JP, Sushila S, Kalia AN, Dangi AS. Hypoglycaemic and hypolipidemic activity of ethanolic extract of Salvadora oleoides in normal and alloxan-induced diabetic rats. Ind J Pharm 2008;40:23-7.

Radhika S, Smila KH, Muthezhilan R. Antidiabetic and hypolipidemic activity of Punica granatum Linn on alloxan induced rats. World J Med Sci 2011;6:178-82.

Ojewale AO, Olaniyan OT, Yemitan OK, Odukanmi OA, Dare BJ, Akingbade AM, et al. Antihypoglycemic and antihypolipidemic activities of ethanolic roots extract of Crossopteryx febrifuga in alloxan-induced diabetic rats. Min J Pharm Med Sci 2013;2:48-51.

Mamun-or-Rashid AMN, Hossain MS, Hassan N, Dash BKD, Sapon MA, Sen MK. A review on medicinal plants with antidiabetic activity. J Pharmacogn Phytochem 2014;3:149-59.

Thompson Coon JS, Earnst E. Herbs for serum cholesterol reduction systemic review. J Farm Pract 2003;52:468-78.

Mohammad A, Kumar D, Rizvi SI. Antidiabetic potential of some less commonly used plants in traditional medicinal systems of India and Nigeria. J Int Ethnopharmacol 2015;4:78-85.

Maniyar Y, Bhixavatimath P. Antihyperglycemic and hypolipidemic activities of aqueous extract of Carica papaya linn. leaves in alloxan-induced diabetic rats. J Ayur Int Med 2012;3:70-4.

Endrini S. Antioxidant activity and anticarcinogenic properties of “Rumput Mutiara” {Hedyotis corymbosa (L.) Lam.} and “pohpohan” {Pilea trinervia (Roxb.)Wight}. J Med Plants Res 2011;5:3715-8.

Chimkode R, Patil MB, Jalapure S, Pasha TY, Sarkar S. A study of hepatoprotective activity of Hedyotis corymbosa. Linn in albino rats. Anc Sci Life 2009;28:32-5.

Fatema UK, Hossain MS. Analgesic effect of ethanol extract of Hedyotis corymbosa whole plant. Int Res J Pharm 2014;5:21-4.

Yuan YV, Bone DE, Carrington MF. Antioxidant activity of dulse (Palmaria palmata) extract evaluated in vitro. Food Chem 2005;91:485-94.

Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. J Med Plants Res 2009;3:67-72.

Stankovic MS. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. J Sci 2011;33:63-72.

Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36:1090-2097.

Szkudelski T. The mechanism of alloxan and streptozotocin action in beta cells of the rat pancreas. Physiol Res 2001;50:536-46.

Kumar RP, Sujatha D, Saleem TSM, Chetty CM, Ranganayakulu D. Potential antidiabetic and antioxidant activities of Morus indica and Asystasia gangetica in alloxan-induced diabetes mellitus. J Exp Pharm 2010;2:29-36.

Jollow DJ, Thorgeirsson SS, Potter WZ, Hashimoto M, Mitchell JR. Acetaminophen-induced hepatic necrosis II. Role of covalent binding in vivo. J Pharm Exp Ther 1974;187:195-202.

Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzy 1978;52:302-10.

Claiborne AL. Catalase activity. CRC handbook of methods for oxygen radical research; 1985. p. 283-4.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–75.

Chede PS. Phytochemical analysis of Citrus sinensis peel. Int J Pharma Bio Sci 2013;4:339-43.

Mishra C, Singh B, Singh S, Siddiqui MJA, Mahdi AA. Role of phytochemicals in diabetes lipotoxicity: an overview. Int J Res Dev Pharma Life Sci 2015;4:1604-10.

Balamurugan K, Nishanthini A, Mohan VR. Antidiabetic and antihyperlipidaemic activity of ethanol extract of Melastoma malabathricum linn. leaf in alloxan induced diabetic rats. Asian Pac J Trop Biomed 2014;4:442-8.

Khan TY, Raina R, Verma PK, Sultana M, Jyoti MK. Phytochemical constituents and antidiabetic potential of Ipomoea carnea leaves extracts. J Exp Integ Med 2014;4:137-42.

Ragini V, Prasad KV, Bharathi K. Antidiabetic and antioxidant activity of Shorea tumbuggaia rox. Int J Innov Pharmal Res 2011;2:113-21.

Mall GK, Mishra PK, Prakash V. Antidiabetic and hypolipidemic activity of Gymnema sylvestre in alloxan induced diabetic rats. Global J Biotech Biochem 2009;4:37-42.

Zhang Z, Lv G, Pan H, Sandos LFF, Fan M, Soccol CR, et al. Antidiabetic activities of ethanol extract of dry matters of culture broth of Coriolus versiolor in submerged culture. Braz Arch Biol Tech 2011;54:701-8.

Pari L, Latha M. Antidiabetic effect of Scoparia dulcis: effect on lipid peroxidation in streptozotocin diabetes. Gen Physiol Biophys 2005;24:13-26.

Shrestha D, Pandey J, Gyawali C, Lamsal M, Sharma S, Rokaya RK, et al. Study of in vitro antioxidant and antidiabetic activity by Mussandra macrophylla root extract. Intl J Curr Pharm Res 2020;12:70-4.

Indradevi S, Havenil S, Kaleeswaran B, Srigopalram S, Ravikumar S. Ethanolic extract of Crinum asiaticum attenuates hyperglycaemia-mediated oxidative stress and protects hepatocytes in alloxan induced experimental diabetic rats. J King Saud Uni Sci 2010;24:171-7.

Bako HY, Mohammad JS, Waziri PM, Gwarzo MY, Zubairul MM. Lipid profile of alloxan-induced diabetic wistar rats treated with methanolic extract of Adansonia digitata fruit pulp. Sci World J 2014;9:19-24.



How to Cite

ELWON, C. A. M., M. D. SHAH, and M. IQBAL. “HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF OLDENLANDIA CORYMBOSA AGAINST ALLOXAN INDUCED DIABETES MELLITUS IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 12, no. 10, Oct. 2020, pp. 34-40, doi:10.22159/ijpps.2020v12i10.38951.



Original Article(s)