PHYSIOCHEMICAL INVESTIGATION AND BIOVALUATION OF TiO2 NANOCRYSTALS SYNTHESIZED BY CHEMICAL AND GREEN ROUTE
Keywords:
Antimicrobial, Cytotoxicity, Desmodium gangeticum, Green synthesis, Phytoconstituents, NilAbstract
Objectives: In this present work, we have compared titanium dioxide nanocrystals synthesized by both chemical method and biological method from Titanium tetra isopropoxide as precursor. The biological method was performed by using the aqueous root extract of Desmodium gangeticum and further they were characterized and checked for their biological activities.
Methods: The TiO2 nanocrystals were characterized by UV-Visible spectrophotometer for its maximum SPR peak, X-ray diffractometry for its crystalline size and shape, Fourier transform infrared spectroscopy for functional entity identification and Zeta potential for its colloidal stability. Antioxidant assay and antimicrobial test were performed to evaluate its biological behaviour.
Results: The nanocrystals synthesized by biological method were found to have higher antioxidant potential, antimicrobial activity and phenolic content than the chemically synthesized ones. Evaluation of toxicity in cell line LLC-PK1 suggests that TiO2nanocrystals synthesized by green route is less toxic.
Conclusion: Green synthesized TiO2 nanocrystals were seen to have less toxicity than the chemical method in showing their biocompatibility nature which can be applied in the field of medical science.
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