IN VITRO ANTI-INFLAMMATORY ACTIVITY OF SYRINGIC ACID
DOI:
https://doi.org/10.22159/ijpps.2019v11i2.30387Keywords:
Syringic acid, Antioxidant, Anti-inflammatory, Protein denaturation, Proteinase inhibition, Membrane stabilizationAbstract
Objective: The present study was carried out to investigate the in vitro anti-inflammatory activity of syringic acid (SA).
Methods: SA was tested for it's in vitro anti-inflammatory activity at different concentrations in protein denaturation, proteinase inhibition and human red blood cell (HRBC) membrane stabilization assay. The reference drugs used were aspirin and diclofenac sodium.
Results: SA showed concentration-dependent inhibition of protein denaturation and proteinase activity with a half-maximal inhibitory concentration (IC50) value of 49.38±0.56 µg/ml and 53.73±0.27 µg/ml respectively. Heat-induced haemolysis was inhibited by SA with an IC50 value of 57.13±0.24 µg/ml. SA also inhibited the hypotonicity-induced haemolysis (IC50 value of 53.87±0.72 µg/ml).
Conclusion: From the present study, we can conclude that SA possesses appreciable anti-inflammatory effect against denaturation of proteins, proteinase activity, and human red blood membrane stabilization assays. Further studies are required for determining the possible mechanisms behind its anti-inflammatory action.
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