ATTENUATING EFFECT OF TRITERPENOID SAPONIN RICH FRACTION OF ACHYRANTHES ASPERA LINN. ON ACUTE AND CHRONIC INFLAMMATION IN EXPERIMENTAL RATS
Keywords:
Achyranthes aspera, Anti-inflammatory, Antioxidant, Triterpenoid saponinsAbstract
Objective: Achyranthes aspera Linn. is used as a traditional remedy for the treatment of various inflammatory conditions in India. The present study was designed to investigate the anti-inflammatory activity of methanolic extract of Achyranthes aspera (AA) and its active fraction using bioassay guided fractionation.
Methods: The dry whole plant of AA was extracted with methanol and then fractionated with different polarity of solvents. Bioactive petroleum ether fraction was re-fractionated into triterpenoid saponin rich (TSR) and non-saponin subfractions and tested for anti-inflammatory activity. The activity of TSR subfraction was evaluated against oxidative stress induced by carrageenan in rat paw tissues.
Results: TSR fraction showed significant (p<0.05) inhibition of rat paw oedema volume. Moreover, TSR fraction was also found to attenuate carrageenan induced oxidative damage by improving antioxidant enzymes levels. Furthermore, TSR fraction inhibited both heat and hypotonicity induced haemolysis of erythrocytes in vitro. In addition, TSR fraction significantly (p<0.05) reduced the granuloma formation in cotton pellet-induced granuloma in rats.
Conclusions: The present study indicates TSR is a potential therapeutic for the treatment of inflammation-associated disorders.
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