PROXIMATE COMPOSITION, NUTRITIVE VALUE AND EVALUATION OF ANTIOXIDANT POTENTIAL OF STEM OF DRACAENA REFLEXA LAM
Keywords:
Dracaena reflexa, Phytochemical, Proximate composition, Nutritive value, Total phenol, Antioxidant potentialAbstract
Objective: The stem of Dracaena reflexa was analyzed for preliminary phytochemical screening, proximate composition, nutritive value, total phenolic content and antioxidant potential.
Methods: Preliminary phytochemical screening, proximate analysis, nutritive value were evaluated using standard methods. Total phenols were calculated by folin ciocalteu method. The antioxidant activity was performed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing antioxidant power (FRAP) assay.
Results: Phytochemical screening revealed the presence of various medicinal active phytoconstituent like alkaloids, flavonoids, glycosides, tannins etc. Total phenolic content was highest for dichloromethane extract (73.48mGAE/g dry weight). Proximate analysis showed content of ash (8.015%), moisture (3.31%), fat (0.97%), fiber (1.31%), protein (3.70%), carbohydrate (86.01%) and nutritive value (367.56 Kcal/100grams of stems). In DPPH assay highest activities shown by dichloromethane extract with IC50 values of 0.46 mg/ml and also have FRAP value 2.071.
Conclusion: Overall results revealed that Dracaena reflexa stem exhibits excellent nutritive value along with potent antioxidant activity and can be used in medicine as well as in food supplements to prevent the oxidation and rancidity of food.
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Singh P, Shrivastava R, Saxena RC, Sharma M, Karchuli MS, Tripathi J. Phytochemical screening and evaluation of antioxidant activity of Parkinsonia aculeata l. Leaves extract. Int J Pharm Tech Res 2011;3:1952-7.
Janifer X Raj, Chaurasia AP, Vajpayee PK, Pal Murugun M, Singh Shashibala. Antioxidant activity and phytochemical investigation on a high altitude medicinal plant Dracocephalum heterophyllum benth, Pharmacogn J 2010;2:112-7.
Pandey M, Abidi AB, Singh S, Singh RP. Nutritional evaluation of leafy vegetable Paratha. J Hum Ecol 2006;19:155-6.
Gurav Shailendra, Deshkar N, Gulkari V, Durgakar N, Patil A. Free radical scavenging activity of Polyagala chinensis. Pharmacol 2007;2:245-53.
Atanassova M, Georgieva S, Ivancheva K. Total phenolic and total flavonoids contents, antioxidant capacity and biological contaminants in medicinal herbs. J Univ Chem Techn Metal 2011;46:81-8.
Machala M, Kubinova R, Horavova P, Suchy V. Chemoprotective potentials of homoisoflavonoids and chalcones of Dracaena cinnabari: modulations of drug-metabolizing enzymes and antioxidant activity. Phytother Res 2001;15:114-8.
Gupta D, Bleakely B, Gupta RK. Dragon’s blood: botany, chemistry and therapeutic uses. J Ethnopharmacol 2008;115:361-80.
Ahmed A, Sobarry M, Cherrah Y, Alaoui K. Anti-inflammatory and analgesic effects of ethanol extract of Dracaena cinnabari Balf. As endemic plant in Yemen. Int J Pharma Bio Sci 2012;3:96-106.
Kumar VP, Chauhan NS, Harish P, Rajani. Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol 2006;107:182-8.
Gonzalez AG, Hernandez JC, Leon F, Padrona JI, Estevez F, Quintana J, et al. Steroidal saponins from the bark of Dracaena draco and their cytotoxic activities. J Nat Prod 2003;63:1297-9.
Wenli M, Haofu D, Jiao W, Ling Z, Kui H. Study on the new use of antitumor of Dracaena cambodiana. J Chin Med Mat 2005;1:10-4.
Luo Y, Wang H, Xu X, Mei W, Dai H. Antioxidant phenolic compounds of Dracaena cambodiana. Molecules 2010;15:8904-14.
Chen HQ, Zuo WJ, Wang HY, Luo Y, Dai HF, Mei WL. Two new antimicrobial flavones from dragon's blood of Dracaena cambodiana. J Asian Nat Prod Res 2012;14:436-40.
AOAC. The Official Method Of Analysis. Association Of Official Analytical Chemists. 15th Ed. Washington, DC; 1990.
Shukla RK, Painuly D, Porval A, Shukla A. Proximate analysis, nutritive value, total phenolic content and antioxidant activity of Litchi chinensis Sonn. Nat Prod: Indian J 2012;8:361-9.
Shukla Abha, Vats Swati, Shukla RK. Preliminary phytochemical screening, antibacterial and nitric oxide scavenging activities of Reinwardtia indica leaves extract. Int J Pharmtech Res 2013;5:1670-8.
Evans WC: Trease and Evans' Pharmacognosy.16th ed. Elsevier Health Sciences publishers. UK; 2009.
Harborne JB. Phytochemical Methods A Guide To Modern Techniques of Plant Analysis. 4th ed. Springer publishers; 1998.
Singleton VL, Orthofer R, Raventos M Rosa. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin ciocalteu reagent. Methods Enzym 1999;299:152-77.
Sharan SV, Srinivasa RB, Chippada SC, Vangalapati M. In vitro antioxidant activity and estimation of total phenolic content in methanolic extract of Bacopa monnifera. Rasayan J Chem 1999;4:381-6.
Brand Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.
Benzie Iris FF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the trap assay. Anal Biochem 1996;239:70-6.
Nagalingam S, Sasikumar CS, Cherian KM. Extraction and preliminary phytochemical screening of active compounds in Morinda citrifolia fruit. Asian J Pharm Clin Res 2012;5:179-81.
Ramani A, Charles A, Stanely L, Joseph M, Mani C. Novel extraction and phytochemical screening of Alseodaphne semecarpifolia nees. Int J Pharm Pharm Sci 2012;4:86-7.
Malu SP, Obochi GO, Edem CA, Nyong BE. Effect of methods of extraction on phytochemical constituents and antibacterial properties of Tetracarpidium conophorum Seeds. Global J Pure App Sci 2009;15:373-6.
Doss A, Parivuguna V, Vijayasanthi M, Surendran S. Antibacterial evaluation and phytochemical analysis of Medicago sativa against some microbial pathogens. Ind J Sci Tech 2011;4:550-2.
Okunrobo LO, Imafidon Kate E, Alabi AA. Phytochemical, proximate and metal content analysis of the leaves of Psidium guajava. Int J Health Res 2010;3:217-21.
Idris S, Iyaka YA, Ndamisto MM, Paiko YB. Nutritional composition of the leaves and stems of Ocimum gratissimum. J Emerging Trends Eng Appl Sci 2011;2:801-5.
Kochhar A, Nagi M, Sachdeva R. Proximate composition, available carbohydrates, dietary fiber and anti-nutritional factors of selected traditional medicinal plants. J Human Ecol 2006;19:195-9.
Fogbound, Lawal ED, Ore ME. The proximate, mineral and phytochemical analysis of the leaves of Ocimum gratissimum l, Melanthera scandens a. And Leea guineensis l, and their medicinal value. Int J Appl Biol Pharm Technol 2011;3:15-22.
Priyanka B, Joy JM, Kumar GA, Lakshmi SM. Comparative antioxidant activity of Asparagus racemosus with its alternative source Asparagus gonoclado. Int J Phytother Res 2012;2:51-6.
Prasad Chandan, Kumar V, Kamthan KP, Singh UB, Srivastava SK, Srivastava RB. Antioxidant and antimicrobial activity of ethanol and water extracts of Cymbopogon jwarancusa leaves. J Appl Pharm Sci 2011;1:68-72.
Patt DE, Hudson BJF. Food antioxidants; natural antioxidants not exploited commercially, Elsevier applied science; 1990. p. 171-91.