IN-VITRO ANTIMICROBIAL ACTIVITY OF BIOLOGICAL SYNTHESIZED SILVER NANOPARTICLES USING STENOTROPHOMONAS MALTOPHILIA STRAIN NS-24 FROM NON-RHIZOSPHERE SOIL

SREENIVASA NAYAKA; BIDHAYAK CHAKRABORTY; DATTATREYA AIRODGI; MEGHASHYAMA P. BHAT; SHASHIRAJ K. NAGARAJA; PALLAVI S. SWAMY; MUTHURAJ R.; HALASWAMY HIREMATH; DHANYAKUMARA S.  BASAVARAJAPPA; MARIA  REGO

doi:10.22159/ijpps.2020v12i5.37257

Authors

SREENIVASA NAYAKA P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
BIDHAYAK CHAKRABORTY P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
DATTATREYA AIRODGI P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
MEGHASHYAMA P. BHAT P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
SHASHIRAJ K. NAGARAJA P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
PALLAVI S. SWAMY P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
MUTHURAJ R. P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
HALASWAMY HIREMATH P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
DHANYAKUMARA S. BASAVARAJAPPA P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003
MARIA REGO P. G. Department of Studies in Botany, Karnatak University, Dharwad, Karnataka, India 580003

DOI:

https://doi.org/10.22159/ijpps.2020v12i5.37257

Keywords:

Stenotrophomonas maltophilia, AgNPs, XRD, HR-TEM, AFM, Antimicrobial

Abstract

Objective: The present goals of our study were biological synthesis, characterizations of silver nanoparticles, and evaluation of its antimicrobial activity against microbial pathogens like Escherichia coli, Enterococcus faecalis, Streptococcus pneumoniae and Staphylococcus aureus.

Methods: The bacterial Strain NS-24 was isolated on nutrient agar medium and was selected for the synthesis of silver nanoparticles based on its gram-negative characteristics. The characterizations of silver nanoparticles were done by UV-Visible spectroscopy, Atomic Force Microscopy (AFM), High Resolution-Transmission Electron Microscopy (HR-TEM), Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDX), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Later, the molecular characterization of the Strain NS-24 was done by DNA extraction and 16S rRNA gene sequencing.

Results: The UV-visible spectrophotometric observation of the Strain NS-24 supernatant and AgNO₃ solution showed maximum absorbance at 423 nm. The AFM data confirmed that the particles were polydispersed and spherical in shape. Additionally, the FTIR analysis revealed the IR spectral band patterning and TEM analyzes showed the size of biological AgNPs was in the range of 12.56 nm to 27.32 nm, with an average of 18.06 nm in size. Further, the 16S rRNA gene sequencing revealed the identity of Strain NS-24 as Stenotrophomonas maltophilia. The antimicrobial activity of AgNPs was studied on different gram-negative and gram-positive bacterial strains like Escherichia coli (MTCC 40), Enterococcus faecalis (MTCC 6845), Streptococcus pneumoniae (MTCC 8874) and Staphylococcus aureus (MTCC 2825), which showed good inhibition of their growth at varying concentrations of AgNPs against all the pathogens.

Conclusion: Our findings showed that the synthesized AgNPs from the isolated bacterium was small in size and had profound antibacterial activity against pathogenic micro-organisms.

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