MICROBIAL SYNTHESIS OF SILVER NANOPARTICLES USING STREPTOMYCES SP. PG12 AND THEIR CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND CYTOTOXICITY ASSESSMENT AGAINST HUMAN LUNG (A549) AND BREAST (MCF-7) CANCER CELL LINES

PALLAVI S. S.; MEGHASHYAMA PRABHAKARA BHAT; SREENIVASA NAYAKA

doi:10.22159/ijpps.2021v13i8.41876

Authors

PALLAVI S. S. P. G. Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India
MEGHASHYAMA PRABHAKARA BHAT P. G. Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India
SREENIVASA NAYAKA P. G. Department of Studies in Botany, Karnatak University, Dharwad 580003, Karnataka, India

DOI:

https://doi.org/10.22159/ijpps.2021v13i8.41876

Keywords:

Silver nanoparticles, Streptomyces sp, Antimicrobial activity, ROS expression, human lung and breast cancer cell lines

Abstract

Objective: Synthesis of silver nanoparticles using Streptomyces sp. PG12 and their characterization, antimicrobial activity and cytotoxicity against A549 and MCF-7 cancer cell lines.

Methods: The silver nanoparticles were subjected to UV-Vis. spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS), high-resolution transmission electron microscopy (HR-TEM), zeta potential, and X-ray diffractometry (XRD) analyses. Further, the antimicrobial potential was determined by using the agar well diffusion method and cytotoxicity was determined with the help of cell viability (MTT) assay and reactive oxygen species (ROS) assay.

Results: The initial indication of silver nanoparticles synthesis was noticed by the colour change in the reaction mixture and the absorption maximum at 421 nm in UV-Vis. analysis; whereas, the FTIR analysis displayed the biological functional groups responsible for the capping and stabilization of silver nanoparticles. SEM and TEM micrographs revealed the surface morphology, spherical shape, and smallest particle size as 18.91 nm. The EDS and XRD patterns confirmed the involvement of various elements during the synthesis of silver nanoparticles and the crystalline, face-centered cubic nature, respectively. The silver nanoparticles displayed considerable antimicrobial activity against human pathogens even at low MIC and MBC concentrations and exhibited increased anticancer activity against A549 and MCF-7 cell lines, where the ability of silver nanoparticles to significantly restrict the growth of tumour cells was observed at IC₅₀ values of 69.04µg/ml and 138.30µg/ml, respectively.

Conclusion: Streptomyces sp. PG12 synthesized silver nanoparticles show significant anticancer activity against A549 and MCF-7 cell lines.

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