• SARI BEMA RAMDIKA Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
  • ENDANG MAHATI Department of Pharmacology and Therapy, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
  • ANANG MOHAMAD LEGOWO Department of Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia
  • MUFLIHATUL MUNIROH Department of Physiology, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
  • YORA NINDITA Department of Pharmacology and Therapy, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia
  • DIANA NUR AFIFAH Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia



Obesity, Dadih, Vitamin D3, IL-6, SCFA


Objective: This study aims to determine the effect of fortified dadih with vitamin D3 on IL-6 expression level and the concentration of caecum SCFA in obese rats.

Methods: A total of 30 male Sprague Dawley rats were divided into five equal groups: healthy-control-(K-), obese-control-(K+), obese-intervention-(X1, X2, and X3). K(+), X1, X2, and X3 were in obesity conditions, which was induced by a high-fat sucrose diet (HFSD) and K(-) as a healthy-control-group. Furthermore, vitamin D3-fortified dadih at doses of 4 g/200 g-body-weight/d, dadih only at doses of 4 g/200 g-body-weight/d, and vitamin D3 only at 36 IU/200 g-body-weight/d was administered to X1, X2, and X3 groups, respectively.

Results: Treatment using fortified dadih with vitamin D3 showed significantly reduce weight gain (p<0.05) compare to K(+) and X2. In addition, X1 showed a decreased level of Interleukin-6 expression (p<0.05) than K(+), X2, and X3 groups but higher than K(-). Also, it showed the highest total SCFA, acetate, and propionate concentration (p<0.05). However, a moderately negative correlation was discovered between the pair of total SCFA and Interleukin-6 expression, acetate and Interleukin-6 expression, SCFA and body weight, propionate and body weight, butyrate and body weight. On the contrary, a strong positive correlation was observed between the pair of Interleukin-6 expression levels and body weight.

Conclusion: This study shows that fortified dadih with vitamin D3 from fermented foods improve the expression level of Interleukin-6 and increase the production of SCFA. Also, they improve intestinal homeostasis because of the increased SCFA production.


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How to Cite

RAMDIKA, S. B., E. MAHATI, A. M. LEGOWO, M. MUNIROH, Y. NINDITA, and D. N. AFIFAH. “FORTIFIED DADIH (FERMENTED BUFFALO MILK) WITH VITAMIN D3 IMPROVES INTERLEUKIN-6 AND CAECUM SHORT CHAIN FATTY ACIDS ON DIET-INDUCED OBESE RAT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 12, no. 11, Nov. 2020, pp. 100-5, doi:10.22159/ijpps.2020v12i11.39209.



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