• EMELIA OPPONG BEKOE Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, College of Health Sciences, University of Ghana
  • MICHAEL WIAFE-KWAGYAN Department of Plant and Environmental Biology, College of Basic and Applied Sciences, University of Ghana
  • JOYCELYN GAYSI Department of Pharmacognosy and Herbal Medicine, School of Pharmacy, College of Health Sciences, University of Ghana



Antibacterial activity, Antibiosis, Aspergillus chevalieri, Trichoderma harzianum, Culture metabolites


Objective: This study sought to preliminarily investigate the inhibitory effect of metabolites of Aspergillus chevalieri and Trichoderma harzianum on a number of pathogenic bacteria.

Methods: The agar well diffusion method was employed to determine the antimicrobial activity of the fungal metabolites. The test microorganisms were Enterococcus faecalis, methicillin-resistant Staphylococcus aureus (MRSA), Salmonella typhi, Escherichia coli and Pseudomonas aeruginosa.

Results: Both metabolites had broad-spectrum antibacterial activity. All the test organisms were susceptible to the A. chevalieri metabolites except for S. typhi. Both S. typhi and E. faecalis were however not susceptible to T. harzianum metabolites. P. aeruginosa was highly susceptible to both metabolites with the highest zone of inhibition of 26 mm for the stock metabolite. This activity was comparable to the standard, 10 µg/ml of ciprofloxacin.

Conclusion: Metabolites of A. chevalieri and T. harzianum exhibited broad-spectrum activity, and this can be exploited as a source for novel antibiotics.


Download data is not yet available.


Gao J, Radwan MM, Leon F, Wang X, Jacob MR, Tekwani BL, et al. Antimicrobial and antiprotozoal activities of secondary metabolites from the fungus Eurotium repens. Med Chem Res 2012;21:3080–6.

Peleg AY, Hooper DC. Hospital-acquired infections due to gram-negative bacteria. N Engl J Med 2010;362:1804–13.

Golkar Z, Bagasra O, Pace DG. Bacteriophage therapy: a potential solution for the antibiotic resistance crisis. J Infect Dev Ctries 2014;8:129–36.

Smith R. The true cost of antimicrobial resistance. Br Med J 2013;346:f1493.

Hancock REW. Mechanisms of action of newer antibiotics for gram-positive pathogens. Lancet Infect Dis 2005;5:209–18.

Bonjar GHS, Fooladi MH, Mahdavi MJ, Shahghasi A. Broadspectrim, a novel antibacterial from streptomyces sp. Biotechnology 2005;3:126–30.

Bosgelmez Tinaz G. Disruption of bacterial cell-to-cell communication (Quorum Sensing): a promising novel way to combat bacteria-mediated diseases. J Marmara Uni Instit Health Sci 2013;3:1.

Zgoda JR, Porter JR. A convenient microdilution method for screening natural products against bacteria and fungi. Pharm Biol 2001;39:221–5.

Clardy J, Fischbach MA, Currie CR. The natural history of antibiotics. Curr Biol 2009;19:437-41.

Karwehl S, Stadler M. Exploitation of fungal biodiversity for discovery of novel antibiotics. Curr Top Microbiol 2016;398:303–38.

Majolagbe ON, Aina DA, Omomowo IO, Thomas A. Antimicrobial potentials of soil fungi metabolites, molecular dynamics and their 3d protein structural prediction using bioinformatics tool. IJCPR 2020;12:20-6.

Wiafe Kwagyan M, Odamtten GT, Obodai M. Possible antibiosis effect of the metabolites of three fungal species resident in rice straw and husk compost on the in vitro radial and vegetative growth by pleurotus ostreatus strain EM-1 and P. eous strain P-31. Int J Curr Microbiol Appl Sci 2015;4:525-38.

Abu AA, Ado SA, James DB. Raw starch degrading amylase production by mixed culture of aspergillus niger and saccharomyces cerevisae grown on sorghum pomace. Afr J Biotechnol 2005;4:785–90.

Ajayi AA, Adejuwon AO, Obasi CK, Olutiola PO, Peter Albert CF. Amylase activity in culture filtrate of aspergillus chevalieri. IJBCS 2014;8:2174–82.

Chen AJ, Hubka V, Frisvad JC, Visagie CM, Houbraken J, Meijer M, et al. Polyphasic taxonomy of Aspergillus (formerly Eurotium), and its occurrence in indoor environments and food. Stud Mycol 2017;88:37–135.

Naidu J, Singh SM. Aspergillus chevalieri (Mangin) thom and church: a new opportunistic pathogen of human cutaneous aspergillosis: Aspergillus chevalieri (Mangin) Thorn und Church: Ein neuer opportunistischer erreger von kutaner. Aspergillose Beim Menschen Mycoses 1994;37:271–4.

Bramki A, Ghorri S, Jaouani A, Dehimat L, Kacem CN. Antibacterial activity of aspergillus isolated from different algerian ecosystems. Afr J Biotechnol 2017;16:1699–704.

Leelavathi MS, Vani L, Reena P. Antimicrobial activity of Trichoderma harzianum against bacteria and fungi. Int J Curr Microbiol Appl Sci 2014;3:96–103.

Kucey RMN, Janzen HH, Leggett ME. Microbially mediated increases in plant-available phosphorus. Adv Agron 1989;42:199–228.

Hermosa R, Cardoza RE, Rubio MB, Gutierrez S, Monte E. Secondary metabolism and antimicrobial metabolites of trichoderma. Biotechnology and Biology of Trichoderma; 2014. p. 125-37.

Agyare C, Bempah SB, Boakye YD, Ayande PG, Adarkwa Yiadom M, Mensah KB. Evaluation of antimicrobial and wound healing potential of Justicia flava and Lannea welwitschii. Evid Based Complement Alternat Med 2013;2013:1-0.

Agyare C, Koffuor GA, Boamah VE, Adu F, Mensah KB, Adu-Amoah L. Antimicrobial and anti-inflammatory activities of Pterygota macrocarpa and Cola gigantea (Sterculiaceae). Evid Based Complementary Altern Med 2012:1-9. 10.1155/2012/902394.

Gbedema SY, Emelia K, Francis A, Kofi A, Eric W. Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant. Pharmacogn Res 2010;2:63-8.

Payne DJ, Gwynn MN, Holmes DJ, Pompliano DL. Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nat Rev Drug Discovery 2007;6:29–40.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 y from 1981 to 2010. J Nat Prod 2012;75:311–35.

Jakubczyk D, Dussart F. Selected fungal natural products with antimicrobial properties. Molecules 2020;25:911.

Nguyen L, Garcia J, Gruenberg K, MacDougall C. Multidrug-resistant pseudomonas infections: hard to treat, but hope on the horizon? Curr Infect Dis Rep 2018;20:23.

Khayat AS, Al-Aaraji AM. The inhibitory effect of trichoderma crude extract against some human pathogenic bacteria. Plant Arch 2020;20:627–32.

Cook R, Baker KF. The nature and practice of biological control of plant pathogens. Am Phytopathol Soc 1983;2:539.



How to Cite

BEKOE, E. O., M. WIAFE-KWAGYAN, and J. GAYSI. “ANTIBACTERIAL ACTIVITY OF THE METABOLITES OF ASPERGILLUS CHEVALIERI AND TRICHODERMA HARZIANUM”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 13, no. 2, Feb. 2021, pp. 67-70, doi:10.22159/ijpps.2021v13i2.39654.



Original Article(s)

Most read articles by the same author(s)