LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY DETERMINATION METHOD OF BENCYCLOQUIDIUM BROMIDE: APPLICATION TO DRUG INTERACTION STUDY IN HUMAN

JANVIER ENGELBERT AGBOKPONTO; LOCONON ACHILLE YEMOA; ASSOGBA GABIN ASSANHOU; RUIJUAN LIU; HABIB GANFON; LI DING

doi:10.22159/ijpps.2021v13i10.5791

Authors

JANVIER ENGELBERT AGBOKPONTO Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China, UFR/Pharmacie, Faculte Des Sciences de la Sante, UAC, Cotonou, BENIN
LOCONON ACHILLE YEMOA UFR/Pharmacie, Faculte Des Sciences de la Sante, UAC, Cotonou, BENIN
ASSOGBA GABIN ASSANHOU UFR/Pharmacie, Faculte Des Sciences de la Sante, UAC, Cotonou, BENIN
RUIJUAN LIU Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China
HABIB GANFON UFR/Pharmacie, Faculte Des Sciences de la Sante, UAC, Cotonou, BENIN
LI DING Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China

DOI:

https://doi.org/10.22159/ijpps.2021v13i10.5791

Keywords:

Bencycloquidium bromide, LC-MS/MS, Pharmacokinetic drug interaction, Paroxetine

Abstract

Objective: This study was conducted to develop a sensitive and effective LC-MS/MS method for the determination of bencycloquidium bromide (BCQB) and its application in pharmacokinetic drug interaction study between BCQB and paroxetine.

Methods: The chromatographic separation was performed on Hedera ODS-2 C18 column with a mobile phase consisted of acetonitrile-10 mmol/l ammonium acetate containing 0.2% acetic acid (33:67, v/v) at 550 μl/min, and the plasma samples were processed using solid-phase extraction. The MS/MS transitions were m/z 330.2 → 142.0 for BCQB and m/z 344.2 → 156.1 for the I. S in positive ESI mode.

Results: The validated method was linear over the concentration range of 2-1200 pg/ml with the correlation coefficient r²>0.998. The intra-and inter-batch precisions of the assay were lower than 8.2% and 9.1%, respectively. The lower limit of quantification (LLOQ) was 2 pg/ml. The stability data at different storage conditions of BCQB were within±5% RE. The mean AUC_0-36 of BCQB was increased by approximately 33%, after the administration of BCQB alone and upon co-administration with paroxetine during the drug interaction study.

Conclusion: The LC-MS/MS method validated in this study was robust, reproducible, accurate, precise and reliable and was successfully applied in the pharmacokinetic drug interaction studies.

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