IDENTIFICATION AND QUANTIFICATION OF PHOSPHODIESTERASE-5 INHIBITOR AS FALSIFIED IN ‘‘NATURAL’’ MALAYSIAN HERBAL APHRODISIACS SOLD IN SOME BENINESE MARKETS

ASSOGBA GABIN ASSANHOU; HABIB GANFON; JANVIER ENGELBERT AGBOKPONTO; CYNTHIA TAKPONON; PARFAIT DOFFON; KANDITCHA N’OUENI; LOCONON ACHILLE YEMOA; AHOKANOU FERNAND GBAGUIDI

doi:10.22159/ijpps.2022v14i7.44099

Authors

ASSOGBA GABIN ASSANHOU Laboratoire de Pharmacie Galenique et de Technologie Pharmaceutique https://orcid.org/0000-0003-2537-7835
HABIB GANFON Laboratoire de Pharmacognosie et de Phytotherapie https://orcid.org/0000-0002-1815-154X
JANVIER ENGELBERT AGBOKPONTO Laboratoire de Chimie Analytique et d’Analyse des Médicaments https://orcid.org/0000-0003-3758-5447
CYNTHIA TAKPONON Laboratoire de Pharmacognosie et de Phytotherapie https://orcid.org/0000-0003-3179-8580
PARFAIT DOFFON Laboratoire de Chimie Analytique et d’Analyse des Médicaments
KANDITCHA N’OUENI Laboratoire de Pharmacognosie et de Phytotherapie
LOCONON ACHILLE YEMOA Laboratoire de Chimie Analytique et d’Analyse des Médicaments
AHOKANOU FERNAND GBAGUIDI Medicinal Organic Chemistry Laboratory, UFR Pharmacie, Faculté des Sciences de Santé, Université d’Abomey-Calavi, Campus de Champ de Foire, 01BP188 Cotonou-Benin

DOI:

https://doi.org/10.22159/ijpps.2022v14i7.44099

Keywords:

Herbal aphrodisiacs, Falsification, PDE-5 Inhibitors

Abstract

Objective: Nowadays, there have been several reports of herbal products falsified with well-known synthetic molecules, leading to harmful health consequences for the consumer. The aim of this study was to assess the profile of ‘natural' herbal aphrodisiacs in the local markets of the municipalities of Cotonou and Abomey-Calavi in Benin and to screen some of them for the presence of additives such as sildenafil and tadalafil.

Methods: A non-probability survey was conducted to identify the available aphrodisiacs and their characteristics. Some of them were then selected for analysis. Thin Layer Chromatography (TLC) was adopted for qualitative detection. The TLC positive extracts were then analyzed by HPLC on a C18 column with a mobile phase consisting of a mixture of 0.05M phosphate buffer (pH 5.8), acetonitrile and methanol (30:50:20). The Detection was performed at 290 nm.

Results: Seventy-seven aphrodisiacs were identified and from these, 18 were selected for analysis. Six of them were adulterated with tadalafil. The concentration of tadalafil in the samples was 1.7 to 4.6 times higher than the recommended dose of 20 mg.

Conclusion: This work opens the door to the need to control ‘‘natural’’ labeled products in order to ensure their quality.

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