• Arvind Mishra Medicinal and Process Chemistry
  • Sudeep Gautam Medicinal and Process Chemistry
  • Savita Pal Medicinal and Process Chemistry
  • Akansha Mishra Medicinal and Process Chemistry
  • Arun Rawat Kumar Medicinal and Process Chemistry
  • Rakesh Maurya Medicinal and Process Chemistry
  • Arvind Kumar Srivastava CSIR-Central Drug Research Institute


Hyperglycemia, Diabetes, Momordica charantia, Streptozotocin, High-fructose diet


Objective: The present study was undertaken to assess the effect of Momordica charantia fruits on diabetes mellitus and its related complications.

Methods: Crude powder, aqueous, 95% and 50% ethanol extract of Momordica charantia fruits were administered to normal rats post sucrose load and streptozotocin-induced (STZ-induced) diabetic rats in single-dose study. The aqueous extract was further fractionated into butanol and aqueous fractions which were evaluated for the antihyperglycemic activity at a single dose in STZ-induced diabetic rats. The multiple dose effect of aqueous fraction was studied in high-fructose diet fed (HFD) rats and STZ-induced diabetic rats. The effect of the same fraction on cellular glucose uptake and insulin-signaling was studied in rat skeletal muscle cells (L6).

Results: The aqueous extract of Momordica charantia fruits showed significant lowering of postprandial hyperglycemia in normal rats and also lowered blood glucose level in STZ-induced diabetic rats. The butanol and aqueous fractions also significantly declined the blood glucose level of STZ-induced diabetic rats in single-dose administration with comparatively higher activity in aqueous fraction. In a multiple - dose study the aqueous fraction significantly improved fasting blood glucose, oral glucose tolerance, plasma insulin level, lipid profile, hepatic and renal parameters in both high-fructose diet fed and STZ-induced diabetic rats. In vitro study of L6 cells revealed that the aqueous fraction significantly increased glucose uptake by treated cells by upregulating the expression of IRS, AKT and GLUT4 at both mRNA and protein level.

Conclusion: It can be concluded that the aqueous fraction of aqueous extract of Momordica charantia fruits is competent in the control of diabetes and its related complications.



Download data is not yet available.

Author Biography

Arvind Kumar Srivastava, CSIR-Central Drug Research Institute

Senior Principal Scientist & HOD, Division of Biochemistry


Stanko P, Holla LI. Bidirectional association between diabetes mellitus and inflammatory periodontal disease. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 2014;158(1):35-8.

Alhazmi A, Stojanovski E, McEvoy M, Garg ML. The association between dietary patterns and type 2 diabetes: a systematic review and meta-analysis of cohort studies. J Hum Nutr Diet 2014;27:251-60.

Konate K, Yomalan K, Sytar O, Zerbo P, Brestic M, Patrick VD, et al. Free radicals scavenging capacity, antidiabetic and antihypersensitive activities of flavonoid-rich fractions from leaves of Trichilia emetica and Opilia amentacea in an animal model of type 2 diabetes mellitus. J Evid Based Complimentary Altern Med 2014; http://dx.doi.org/10.1155/2014/867075.

Shrivastava SR, Shrivastava PS, Ramasamy J. Role of self-care in management of diabetes mellitus. J Diabetes Metab Disord 2013;12(14):1-5.

Kodiatte TA, Manikyam UK, Rao SB, Jagadish TA, Reddy M, Lingaiah HKM, et al. Mean platelet volume in type 2 diabetes mellitus. J Lab Physicians 2012;4(1):5-9.

Cade WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. J Am Phys Ther Assoc 2008;88(11):1322-35.

Zhang M, Zhang L, Wu B, Song H, An Z, Li S. Dapagliflozin treatment for type 2 diabetes: a systematic review and meta-analysis of randomized controlled trials. Diabetes Metab Res Rev 2014;30:204-21.

Inzucchi SE, Bergenstal RM, Buse JB, Diamant M, Ferrannini E, Nauck M, et al. Management of hyperglycemia in type 2 diabetes: a patient-centered approach. Diabetes Care 2012;35:1364-79.

Pandey A, Tripathi P, Pandey R, Srivastava R, Goswami S. Alternative therapies useful in the management of diabetes: A systematic review. J Pharm Bioallied Sci 2011;3(4):504-12.

Rahman MMD, Sayeed MA, Biplab KP, Siddique SA. Antidiabetic and cytotoxic activities of methanolic extract of Tabernaemontana divaricata (L.) leaves in alloxan induced mice. Asian J Pharm Clin Res 2012;5(1):49-52.

Hamissou M, Smith AC, Carter Jr RE, Triplett II JK. Antioxidative properties of bitter gourd (Momordica charantia) and zucchini (Cucurbita pepo). Emirates J Food Agric 2013;25(9):641-7.

Kumar SD, Sharathnath KV, Yogeswaran P, Harani A, Sudhakar K, Sudha P, et al. A medical potency of Momordica charantia. Int J Pharm Sci Rev Res 2010;1(2):95-100.

Patel R, Mahobia N, Upwar N, Waseem N, Talaviya H, Patel Z. Analgesic and antipyretic activities of Momordica charantia Linn fruits. J Adv Pharm Tech Res 2010;1(4):415-8.

Costa JGM, Nascimento EMM, Campos AR, Rodrigues FFG. Antibacterial activity of Momordica charantia (Cucurbitaceae) extracts and fractions. J Basic Clin Pharm 2011;2(1):45-51.

Rahuja N, Mishra A, Gautam S, Tamrakar AK, Maurya R, Jain SK, et al. Antidiabetic activity in flowers of Nymphea rubra. Int J Pharm Sci Rev Res 2013;22(1):121-33.

Srivastava R, Srivastava SP, Jaiswal N, Mishra A, Maurya R, Srivastava AK. Antidiabetic and antidyslipidemic activities of Cuminum cyminum L in validated animal models. Med Chem Res 2011;20(9):1656-66.

Tiwari P, Rahuja N, Kumar R, Lakshmi V, Srivastava MN, Agarwal SC, et al. Search for antihyperglycemic activity of new flora and fauna. Indian J Sci Technol 2008;1(5):1-5.

Divi SM, Bellamkonda R, Dasireddy SK. Evaluation of antidiabetic and antihyperlipedemic potential of aqueous extract of moringa oleifera in fructose fed insulin resistant and STZ induced diabetic wistar rats: a comparative study. Asian J Pharm Clin Res 2012;5(1):67-72.

Patel J, Iyer A, Brown L. Evaluation of the chronic complications of diabetes in a high fructose diet in rats. Indian J Biochem Biophys 2009;46(1):66-72.

Reddy SS, Karuna R, Saralakumari D. Prevention of insulin resistance by ingesting aqueous extract of Ocimum sanctum to fructose fed rats. Horm Metab Res 2008;40:44-9.

Hoehn KL, Hohnen BC, Cederberg A, Wu LE, Turner N, Yuasa T, et al. IRS1-independent defects define major nodes of insulin resistance. Cell Metab 2008;7:421-33.



How to Cite

Mishra, A., S. Gautam, S. Pal, A. Mishra, A. R. Kumar, R. Maurya, and A. K. Srivastava. “EFFECT OF MOMORDICA CHARANTIA FRUITS ON STREPTOZOTOCIN-INDUCED DIABETES MELLITUS AND ITS ASSOCIATED COMPLICATIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 356-63, https://journals.innovareacademics.in/index.php/ijpps/article/view/4436.



Original Article(s)