MOLECULAR IDENTIFICATION OF AEROMONAS SPP ISOLATED FROM PATIENTS WITH DIARRHEA AT SANTA MARIA-RS, BRAZIL
Keywords:
Aeromonas, PCR-RFLP, GastroenteritisAbstract
Objective: The aim of the study was to determine the frequency of Aeromonas spp. in stool samples of outpatients with gastroenteritis attended by clinical laboratories at Santa Maria-RS, Brazil.
Methods: In order to evaluate this frequency, 767 clinical stool samples were processed by conventional methods as preconized, and suspected Aeromonas strains were submitted to molecular characterization by 16SrRNA PCR-RFLP method.
Results: Aeromonas spp. were isolated from 14 (1.8%) of stool cultures and identified as A. caviae (04), A. hydrophila (03), and A. veronii biovar sobria (01) by molecular method. Six strains presented atypical PCR-RFLP patterns, and therefore were identified as Aeromonas spp.
Conclusion: Aeromonas is part of the bacteria associated with diarrhea in Santa Maria-RS, and results indicates that at least 3 Aeromonas species are involved with the disease.
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References
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