ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF UROPATHOGENS AT A TERTIARY CARE HOSPITAL IN CENTRAL INDIA DURING COVID ERA.

ABHISHEK MEHTA; HIRDESH KUMARI GUPTA; KIRAN TRIPATHI

doi:10.22159/ijpps.2023v15i5.47533

Authors

ABHISHEK MEHTA Department of Microbiology, Govt. Medical College, Datia (MP), India https://orcid.org/0000-0003-3682-7600
HIRDESH KUMARI GUPTA Department of Microbiology, Govt. Medical College, Datia (MP), India
KIRAN TRIPATHI Department of Microbiology, Govt. Medical College, Datia (MP), India

DOI:

https://doi.org/10.22159/ijpps.2023v15i5.47533

Keywords:

Urinary tract infection, Antimicrobial susceptibility, Antibiotic policy, Multi-drug-resistance, Empirical therapy, Fungal isolates

Abstract

Objective: The purpose of this study was to study the bacteriological profile of UTI in patients attending the tertiary care hospital and to study the antimicrobial sensitivity pattern of uropathogens.

Methods: This cross-sectional study was conducted after obtaining clearance from the institutional ethics committee. Clean-catch mid-stream urine samples were collected from patients symptomatic of UTIs. Samples were cultured aerobically on CLED agar. Isolates having significant growth (>10⁵CFU/ml) were further processed for identification using standard microbiological techniques and their antimicrobial susceptibility pattern was evaluated by the Standard Kirby Bauer disk diffusion method as per CLSI 2020 guidelines.

Results: A total of 480 urine samples were processed, yielding 174 isolates. Escherichia coli (42.50%) was predominant, followed by Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter spp., Proteus spp., Providencia spp., Enterococcus spp., Citrobacter spp. and Morganella morganii. Gram-positive isolates exhibited high sensitivity towards vancomycin, linezolid, meropenem, and piperacillin tazobactum. Enteric coliforms exhibited high sensitivity towards colistin, meropenem, aminoglycosides, and piperacillin tazobactum. Non-fermenters exhibited high sensitivity towards colistin, meropenem, cefepime, and amoxycillin clavulanate.

Conclusion: The rampant injudicious irrational overuse of antibiotics has led to the emergence of multi-drug resistant bugs, which is posing a serious challenge to clinicians in the management of infections. Developing therapeutic protocols guided by susceptibility profiles for tuning antibiotic therapy regimens is an important strategy in tackling this menace.

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