EVALUATION OF SKELETAL MUSCLE RELAXANT ACTIVITY OF AQUEOUS EXTRACT OF CINNAMOMUM VERUMBARK ON SWISS ALBINO MOUSE

ANAMIKA HAZARIKA; PALLAVI BORDOLOI; GEETAMONI DUTTA; DIPJYOTI DEKA

doi:10.22159/ijpps.2024v16i8.51408

Authors

ANAMIKA HAZARIKA Department of Pharmacology, Jorhat Medical College and Hospital, Assam, India https://orcid.org/0009-0002-0377-4127
PALLAVI BORDOLOI Department of Pharmacology, Tinsukia Medical College and Hospital, Assam, India https://orcid.org/0009-0009-4534-5487
GEETAMONI DUTTA Department of Pharmacology, Jorhat Medical College and Hospital, Assam, India https://orcid.org/0000-0001-7155-3241
DIPJYOTI DEKA Department of Pharmacology, Jorhat Medical College and Hospital, Assam, India

DOI:

https://doi.org/10.22159/ijpps.2024v16i8.51408

Keywords:

Cinnamomum verum, Cinnamomum zeylanicum, Ceylon cinnamon, Rota rod, Actophotometer, Muscle relaxant

Abstract

Objective: To evaluate the muscle relaxant activity of aqueous extract of bark of Cinnamomum verum (synonyms: Cinnamomum zeylanicum, Ceylon cinnamon) [AEBCV] on swiss albino mouse.

Methods: Twenty-two albino mice (25-30g) were taken and divided into four groups. The first group was kept as control (Distilled water), second as the standard (Diazepam) and remaining two groups as test 1 & 2 were given different doses of AEBCV (50 and 100 mg/kg). Skeletal muscle relaxant activity on rotarod and actophotometer were performed. Statistical analysis was carried out by using ANOVA, followed by Tukey’s multiple comparison tests, using GraphPad Prism software. P-value<0.05 was considered significant.

Results: In rotarod test, AEBCV (50mg/kg, 100mg/kg) significantly reduced motor coordination of tested animals when compared to control(P<0.05). Maximum muscle relaxation was observed with 100mg/kg. In actophotometer, AEBCV showed highly significant reduction (P<0.05) in the locomotor activity when compared to control that is 74% and 81%. AEBCV also showed significant reduction when compared to standard.

Conclusions: Our data indicates that AEBCV possesses skeletal muscle relaxant activities.

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References

Skeletal muscle relaxant. Available from: http://www.drugs.com/drugs-class/skeletal-muscle-relaxants.html [Last accessed on 2023 September 4]

Rao PV, Gan SH. Cinnamon: a multifaceted medicinal plant. Evid Based Complement Alternat Med.2014; 2014:642942. doi: 10.1155/2014/642942. Epub 2014 Apr 10. PMID: 24817901; PMCID: PMC4003790.

Bell, Maguelonne Toussaint-Samat (2009). A history of food. Translated by Anthea (Newexpanded ed.). Chichester, West Sussex: Wiley-Blackwell. ISBN 978-1405181198. “Cassia, also known as cinnamon or Chinese cinnamon is a tree that has bark similar to that of cinnamon but with a rather pungent odour.”

Britannica, The Editors of Encyclopedia. “cinnamon”. Encyclopedia Britannica, 11 Aug.2023, http://www.britannica.com/plant/cinnamon. [Last assessed on 2023, September 3].

Nairn JG. Solutions, emulsions, suspension and extracts. In: Gennaro A, Marderosian

AD, Hanson GR, Medwick T, Popovich NG, Schnaare RL. The science and practice of pharmacy, 20th edition Philadelphia, Lippincott Williams and Wilkins; 2000:721-752.

CPCSEA Guidelines for laboratory animal facility. Available from: http://care.edu.in/wp-content/uploads/2020/03/cpcsea.pdf [Last accessed on 2023 September 5]

OECD Guidelines for testing of Chemicals (Internal), France: OECD publishing; 2006, Section 4, Health effects: Test No. 425: Acute oral toxicity: Up and down procedure (Adopted 2006, March 23).

Ghosh MN. Fundamentals of experimental pharmacology. 2nd ed. Kolkata: Scientific Book Agency; 1984. p. 156.

Vogel HG, Vogel WH. Drug discovery and evaluation. 2nd ed. Berlin, Heidelberg, New York: Springer-Verlag; 2002.

Rao KV. Multiple comparision test procedures. In: Rao KV, Balakrishnan N. eds. Biostatistics. 1st edn. New Delhi, India: Jaypee Brothers Medical Publishers 1999: p. 273-8.

Tirumalasetty, Jayasree; Rasamal, Kavitha; Nutalapati, Chandrasekhar; Samreen, Saequa. Evaluation of centrally acting skeletal muscle relaxant activity of aqueous extract of Cinnamomum zeylanicum bark in albino mice. Journal of Dr. NTR University of Health Sciences 1(2): p 94-98, Apr–Jun 2012. | DOI: 10.4103/2277-8632.98345 9. doi: 10.1023/a:1008088323164. PMID: 9950084.

Sahu M, Sahoo N K, Revathi M, Shalini P. Comparative study of centrally acting skeletal muscle relaxant activity of aqueous extract of Cinnamomum zeylanicum bark with methocarbamol on albino mice. International journal of pharmaceutical sciences review and research 2013. ISSM 0976-044X.

Duke JA. Handbook of medicinal herbs. Boca Raton, Fia: CRC Press Inc.;1985. p.33-

Desai A et al. (2023). Exploring the potential of herbal therapy in COVID-19. International Journal of Current Pharmaceutical Research. Vol 15 (Issue 6).

Fang SH, Rao YK, Tzeng YM, Inhibitory effects of flavonol glycosides from Cinnamomum osmophloeum inflammatory mediators in LPS/IFN-gamma-activated murine macrophages, Bioorganic and Medicinal Chemistry letters, 13, 2005, 2381-2388.

Khatoon A, Agarwal SM, Butool S. (2022). Pharmacological evaluation of cinnamaldehyde against DMBA-induced skin cancer in albino mice. Asian Journal of Pharmaceutical and Clinical Research. Vol 15 (Issue 11).