CHEMICAL COMPOSITION OF THE HEXANE EXTRACT OF LEAVES OF AZIMA TETRACANTHA (LAM)
Keywords:
Azima tetracantha, GC-MS, Phytochemicals, Hexane extract, Hexadecanoic acid, Oleic acidAbstract
Objective: In the present study, the phytochemical constituents of hexane extract from Azima tetracantha (AT) leaves were done by using Gas Chromatography Mass Spectrometry analysis technique (GC-MS). Azima tetracantha has been an important medicinal herb being used in tribal medicines since long, but chemical constituents of its bark responsible for the activities are still not studied in depth.
Methods: Azima tetracantha leaves were collected from Ariyalur District, Tamil Nadu. The dried leaf was powdered and was extracted with the solvent hexane by using a Soxhlet apparatus. One microlitre of the extract was subjected to analysis by GC-MS to detect the presence of bioactive compounds present in the leaves of the plant.
Results: The results showed that the leaves of Azima tetracantha contained 47 compounds, of which the major is n-hexadecanoic acid (39.10%) followed by oleic acid (11.54%). Analysis and identification of the presence of the compound in the extract were done by using the database of the National Institute of Standards and Technology (NIST) library.
Conclusion: In the present study, 47 chemical constituents have been identified from the hexane leaf extract of Azima tetracantha by GC-MS analysis. The hexane extract is mainly composed of terpenoids and sterols. Thus, Azima tetracantha is found to possess significant phytonutrients, which attribute to its medicinal worth.
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