GENETIC SUSCEPTIBILITY OF HCV RNA IN VARIOUS ISONYM GROUPS OF PUNJAB, PAKISTAN
Keywords:HCV, Genotypes, Genetic, Polymorphism
Objective: To uncover the ecology and genetic factors affecting the susceptibility of infectious diseases of HCV RNA and to understand the role of genetic characters in the outcome and pathogenesis of hepatitis C virus (HCV) infection.
Methods: A total of 349 patients with chronic hepatitis have been included in this genetic susceptibility study. The infected individuals were diagnosed anti-HCV positive and then confirmed by HCV Polymerized Chain Reaction (PCR) qualitative test. All these selected patients had been diagnosed for HCV and taken the standard treatment of interferon and ribavirin from 20 w to 36 w. The end product of PCR was confirmed by gel electrophoresis. The following restriction enzymes Ear I, Rsa 1 and Mae III were used to digest the PCR product. This study focused only on patients who were non-responsive and had relapsed from conventional therapy.
Results: Our study proposes that both the interleukin genes IL-10 and IL-28B are found common in two major castes of the Punjab. We determined that HCV genotype 3a is very common (84.0%) among the group of responders whereas genotype 1a is more familiar in relapse (66.2%) as well as non-responders (54.0%). Excluding Genotype 4, the rest five main genotypes, namely 1a (61.40%), 2a (0.50%), 2b (20.00%), 3a (13.70%) and an untraceable (4.40%) were found among the 12 different castes/tribes ethnic groups I Pakistan.
Conclusion: To better manage and improve the fact behind understanding human susceptibility to HCV infection, a cohort study is beneficial.
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