ANTIOXIDANT AND FREE RADICAL SCAVENGING CAPACITY OF RED SEAWEED HYPNEA VALENTIAE FROM RAMESHWARAM COAST TAMIL NADU, INDIA

Authors

  • Revathi D. Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
  • Baskaran K. Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu, India
  • Subashini R. Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu, India

Keywords:

Hypnea valentiae, Free radical scavenging, Antioxidant activity

Abstract

Objectives: In this study, In-vitro antioxidant and free radical scavenging activity of Hypnea valentiae was evaluated in a series of in vitro assays. Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress.

Methods: The evaluation of antioxidant properties was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis 3ethylbenzthiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), nitric oxide scavenging assay (NO), reducing power, superoxide, hydroxyl radical scavenging and hydrogen peroxide radical assay, the metal chelating activity as well as phosphomolypdenum assay.

Results: Among the tested red algae, the maximum antioxidant activity was recorded in the methanol extract of Hypnea valentiae. Whereas methanol crude extract of red algae and diatom showed good antioxidant potential.

Conclusions: The findings of the present study suggest that all seaweed extracts could be a potential source of natural antioxidant that could have great importance as therapeutic agents.

 

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Author Biography

Subashini R., Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu, India

Assistant Professor
Department of Biochemistry and Biotechnology,
Annamalai University

References

Nagai T, Yukimoto Y. Preparation and functional properties of beverages made from sea algae. Food Chem 2003;81:327-32.

Nakai M, Kagaeyama N, Nakahara K, Mild W. Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum. Mar Biotechnol 2006;8:409-14.

Batista Gonzalez AE, Charles MB, Mancini-Filho J, Vidal Novoa A. Seaweeds as sources of antioxidant phytomedicines. Rev Cubana Plant Med 2009;14:1–18.

Chanda S, Dave R, Kaneria M, Nagani K. Seaweeds: a novel, untapped source of drugs from sea to combat infectious diseases. Curr Res Technol Edu Topics Appl Microbial Biotechnol 2010;1:473-80.

Wijeratne SSK, Cuppett SL, Schlegel V. Hydrogen peroxide induced oxidative stress damage and antioxidant enzyme response in Caco-2 human colon cells. J Agric Food Chem 2005;53:8768–74.

Cox S, Abu-Ghannam N, Gupta S. An assessment of antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int Food Res J 2010;17:205-20.

Patra JK, Rath SK, Jena K, Rathod VK, Thatoi H. Evaluation of antioxidant and antimicrobial activity of seaweeds (Sargassum sp.) extract: a study of inhibition of glutathione–s-transferase activity. Turk J Biol 2008;32:119-25.

Garg HS, Sharma T, Bhakun DS, Pramanik BN, Bose AK. An antiviral sphingosine derivative from green alga Ulva fasciata. Tetrahedron Lett 1992;33:1641-4.

Radhika D, Veerabahu C, Priya R. In vitro studies on antioxidant and haemagglutination activity of some selected seaweeds. Int J Pharm Pharm Sci 2013;5(1):152-5.

Pramitha VS, Lipton AP, Thangaraj M. Evaluation of glucosidase inhibitoryproperties of marriage using intestinal extract of marine snail, Thais rudolphi (Lamarck, 1822). Indian J Biotechnol 2008;7:61-5.

Pramitha VS, Lipton AP. Selective cytotoxicity of macroalgal extracts to Artemia salina (Brine shrimp). Seaweed Res Utiln 2012;34:100-7.

Subhashini N, Nagarajan G, Kavimani S. In vitro antioxidant and anticholinesterase activities of Garcinia combogia. Int J Pharm Pharm Sci 2011;3:129-32.

Huang MH, Huang SS, Wang BS. Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo. J Ethnopharmacol 2011;133:743-50.

Xu W, Zhang F, Luo Y, Ma L, Kou X, Huang K. The antioxidant activity of a water-soluble polysaccharide purified from Pteridium aquilinum. Carbo Res 2009;344:217-22.

Sakat SS, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146-55.

Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 1995;43:27–32.

Chou HG, Kuo JT, Lin ES. Comparative antioxidant properties of water extracts from different parts of Beefsteak plant (Perilla frutescens). J Food Drug Anal 2009;17:489-96.

Naskar S, Islam A, Mazumder UK. In vitro and in vivo antioxidant potential of hydro methanol extracts of phoenix dactylifera fruits. J Sci Res 2010;2:144-57.

Delpour AA, Ebrahimzadeh MA, Nabawi SF, Nabavi SM. Antioxidant activity of the methanol extract of Ferula asafetida and its essential oil composition. Grassy Acts 2009;60:405-12.

Smirnoff N, Cumbes QJ. Hydroxyl radical scavenging activity of compatible solutes. Phytochem 1989;28:1057-60.

Sahaa MR, Hasana SMR, Aktera R, Hossaina MM, Alamb MS, Alam MA, et al. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusopselengi Linn. Bangl J Vet Med 2008;6(2):197–02.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841–56.

Baskaran K, Pugalendi KV, Saravanan R. Free radical scavenging activity of ethyl acetate extract of Cardiospermum halicacabum by In vitro assays. AJBPR 2014;2:300-8.

Subashini R, Prasanth R. Studies of free radicals scavenging potential of seaweed Enteromorpha sp. Chem Sci Rev Lett 2014;3(12):931-40.

Ou B, Huang D, Hampscha–Woodili M, Flanagan J, Deemerr E. Analysis of antioxidant activities of common vegetables, employing oxygen radical absorbance capacity (ORAC) and FRAP assays: a comparative study. J Agric Food Chem 2002;50:3122–8.

Moncada S, Palmer RM, Higgs EA. Nitric oxide physiology pathophysiology and pharmacology. Pharmacol Rev 1991;43:109-42.

Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: an overview. Afr J Microbiol Res 2009;3(13):981-96.

Ferreira I. Baptista CP, Boas MV, Barros L. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stop activity. Food Chem 2007;100:1511-6.

Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Pharm Sci 2011;3(3):126-8.

Sarikurkc C, Tepe B, Semiz DK, Solak MH. Evaluation of metal concentration and antioxidant activity of three edible mushrooms from Mugla, Turkey. Food Chem Toxicol 2010;48:1230-3.

Two-tone M, Phromkunthong W. Antioxidant activities of four edible seaweeds from the southern coast of thailand. Plant Foods Hum Nutr 2009;64:218-23.

Aruoma OI. Free radicals oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75(2):199-212.

Ma X, Li H, Dong J, Qian W. Determination of hydrogen peroxide scavenging activity of phenolic acids by employing gold nanoshells precursor composites as nanoprobes. Food Chem 2011;126:698-704.

Heo SJ, Park EJ, Lee KW, Jeon YJ. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresour Technol 2005;96:1613-23.

Gupta S, Abu-Ghannam N. Bioactive potential and possible health effects of edible brown seaweeds. Trends Food Sci Technol 2011;22:315-26.

Halliwell B. Reactive oxygen species in living systems Source biochemistry and role in human disease. Am J Med 1991;91:14–22.

Gordon MF. The mechanism of antioxidant action in vitro. In: Food Antioxidant BJF Hudson. Ed. Elsevier Applied Science, London: 1990. p. 1–18.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337–41.

Loo A, Jain YK, Sarah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem 2008:107:1151–60.

Published

01-08-2015

How to Cite

D., R., B. K., and S. R. “ANTIOXIDANT AND FREE RADICAL SCAVENGING CAPACITY OF RED SEAWEED HYPNEA VALENTIAE FROM RAMESHWARAM COAST TAMIL NADU, INDIA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 8, Aug. 2015, pp. 232-7, https://journals.innovareacademics.in/index.php/ijpps/article/view/5969.

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