• Shariful Haque Department of Pharmacy, Pabna University of Science and Technology, Pabna 6600, Bangladesh
  • Shatabdi Dey Lopa Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh
  • Biplab Kumar Das Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh


Spilanthes paniculata, Phytochemical screening, Flavonoid, Antioxidant


Objective: The present study was conducted to evaluate the antioxidant potential of Spilanthes paniculata.

Methods: The antioxidant activity of three different extracts (ethanol, methanol and chloroform extracts) of the whole plant was evaluated by determining total antioxidant capacity using phospho molybdenum method and total flavonoid content by Aluminum chloride colorimetric method.

Results: Preliminary results from phytochemical screening showed that ethanol, methanol and chloroform extracts of S. paniculata contain flavonoids along with other phyto chemicals in moderate to high amounts. The extracts were then evaluated for total antioxidant capacity using ascorbic acid as the standard. The total antioxidant capacity of ethanol, methanol and chloroform extracts was found to be 153.54±1.343, 157.97±6.713 and 200.38±4.028 mg/g of plant extract which were expressed as Ascorbic Acid Equivalents (AAE). Also total flavonoid content was determined and was expressed as quercetin equivalents (QE). The content of flavonoid compounds in ethanol, methanol and chloroform extract was estimated to be 101.58±55.919, 121±74.318 and 51.07±19.121 mg/g dry weight of extract.

Conclusion: Based on these findings, it can be concluded that the significant antioxidant potential of the extracts might be attributed to moderate to high amount of flavonoids present in the extract.



Download data is not yet available.

Author Biography

Biplab Kumar Das, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh

Professor, Department of Pharmaceutical Chemistry


Jansen RK. The systematics of Acmella (Asteraceae-Heliantheae). Systematic Botany Monographs 1985b;8:1–115.

Pandey V, Agrawal V, Raghavendra K, Dash AP. Strong larvicidal activity of three species of Spilanthes (Akarkara) against malaria (Anopheles stephensi Liston, Anopheles culicifacies, Species C) and filaria vector (Culex quinquefasciatus Say). Parasitol Res 2007;102:171–4.

Prachayasittikul S, Suphapong S, Worachartcheewan A, Lawung R, Ruchirawat S, Prachayasittikul V. Bioactive metabolites from Spilanthes acmella Murr. Molecules 2009;14:850–67.

Nakatani N, Nagashima M. Pungent alkyl amides from Spilanthes acmella L. var. oleracea Clarke. Biosci Biotechnol Biochem 1992;56:759–62.

Tominaga H, Kobayashi Y, Goto T, Kasemura K, Nomura M. DPPH Radical scavenging effect of several phenylpropanoid compound and their glycoside derivatives. Yakugaku Zasshi 2005;125(4):371–5.

Cao G, Srfie E, Prior RL. Antioxidant capacity of tea and common vegetables. J Agric Food Chem 1996;44:3425–31.

Kaur C, Kapoor HC. Antioxidant activity and phenolic content of some Asian vegetables. Int J Food Sci Technol 2002;37:153–61.

Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F. Screening of antioxidant activity of three Indian medicinal plants traditionally used for the management of neurodegerative diseases. J Ethnopharmacol 2003;84:131–8.

Das BK, Bepary S, Datta BK, Rouf ASS, Ali MS, Chowdhury AKA. Hepatoprotective activity of Phyllanthus reticulatus. Pak J Pharm Sci 2009;22(1):333–7.

Harborne JB. Phytochemical Methods: A guide to modern techniques of plant analysis. 3rd ed. London: Chapman and Hall; 1998. p. 302.

Sazada S, Verma A, Rather AA, Jabeen F, Meghvansi MK. Preliminary phytochemicals analysis of some important medicinal and aromatic plants. Adv Biol Res 2009;3:188–95.

Jamuna KS, Ramesh CK, Srinivasa TR, Raghu KL. In vitro antioxidant studies in some common fruits. Int J Pharm Pharm Sci 2011;3(1):60–3.

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178–82.

Dudonne S, Vitrac S, Coutiere P, Woillez M, Merillon JM. Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD and ORAC assays. J Agric Food Chem 2009;57:1768–74.

Cioffi G, D’Auria M, Braca A, Mendez J, Castillo A, Morelli I, et al. Antioxidant and free radical scavenging activity of constituents of the leaves of Tachigalia paniculata. J Nat Prod 2002;65:1526–9.

Shimada K, Fujikawa K, Yahara R, Nakamura T. Antioxidative properties of xanthan on autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945–8.

Yen GC, Duh PD, Su HJ. Antixodiant properties of lotus seed and its effect on DNA damage in human lymphocytes. Food Chem 2005;89:379–85.

Saumya SM, Mahaboob BP. In vitro evaluation of free radical scavenging activities of Panax ginseng and Lagerstroemia speciosa: A comparative analysis. Int J Pharm Pharm Sci 2011;3(1):165–9.



How to Cite

Haque, S., S. D. Lopa, and B. K. Das. “EVALUATION OF ANTIOXIDANT POTENTIAL OF SPILANTHES PANICULATA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 8, Aug. 2015, pp. 390-2, https://journals.innovareacademics.in/index.php/ijpps/article/view/6703.



Short Communication(s)

Most read articles by the same author(s)