• Pankajavalli Thirugnanasambantham Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore 641043, India
  • Kalaiselvi Senthil Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore 641043, India
  • Taek-joo Oh College of Pharmacy, Chung-Ang University, Seoul 156756, Republic of Korea
  • Hyung Kyoon Choi College of Pharmacy, Chung-Ang University, Seoul 156756, Republic of Korea


Withania somnifera, In vitro leaf, Field grown, GC-MS, GABA, Putrescine


Objective: Metabolomic profiling of herbal extracts is indispensable to standardize drugs and to inaugurate the scientific basis of their therapeutic properties. The present study was attempted with an objective to investigate a comparative GC-MS (Gas chromatography–Mass spectrometry) analysis of in vitro and field grown leaf tissues of “Indian ginseng”.

Methods: GC-MS often serves the methods of option for screening and quantitative metabolite profiling. In the present study, metabolic profiling of methanolic extracts of field and in vitro cultured Withania somnifera (Ashwagandha) leaf tissues were carried out using GC–MS technique.

Results: A total number of 39 primary metabolites in leaf were identified. These include alcohols, organic acids, purine, pyrimidine, sugars and putrescine. Highly significant qualitative and quantitative differences were noticed between the leaf tissues cultured in vitro and from the field. Especially, significant elevation in the accumulation of GABA (γ amino butyric acid) and putrescine was recorded in in vitro cultured leaf samples.

Conclusion: We conclude that in vitro cultures offers an intrinsic advantage to produce therapeutically valuable compounds, relatively in a short span of time and this principle determine its use as an alternative to field grown sample.


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Archana R, Namasivayam A. Antistressor effect of Withania somnifera. J Ethanopharmacol 1999;64:91–3.

Davis L, Kuttan G. Effect of Withania somnifera on DMBA induced carcinogenesis. J Ethanopharmacol 2001;75:165–8.

Kumar A, Kaul MK, Bhan MK, Khanna PK, Suri KA. Withania somnifera (L.) Dunal (Solanaceae). Genet Resour Crop Evol 2007;54:655–60.

Gupta GL, Rana AC. Withania somnifera (Aswagandha): a Review. Pharmacognosy 2007;1:129–36.

Sharma S, Dahanukar S, Karandikar SM. Effect of long-term administration of the roots of ashwagandha and shatvari in rat. Indian Drugs 1985;29:133–9.

Chatterjee S, Srivastava S, Khalid A, Singh N, Sangwan RS, Sidhu OP, et al. Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts. Phytochemistry 2010;71:1085-94.

Ray AB, Gupta M. Withanosteroids, a growing group of naturally occurring steroidal lactones. Prog Chem Org Nat Prod 1994;63:2–106.

Mirjalili MH, Moyano E, Bonfill M, Cusido RM, Palazon J. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 2009;14:2373-93.

Jayaprakasam B, Nair MG. Cyclooxygenase-2 enzyme inhibitory withanolides from Withania somnifera leaves. Tetrahedron 2003;59:841-9.

Ichikawa H, Takada Y, Shishodia S, Jayaprakasam B, Nair MG, Aggarwal BB. Withanolides potentiate apoptosis, inhibit invasion,and abolish osteoclastogenesis through suppression of nuclear factor-EB (NF-EB) activation and NF-EB–regulated gene expression. Mol Cancer Ther 2006;5:1434-45.

Praveen N, Naik PM, Manohar SH, Murthy HN. Distribution of withaolide A content in various organs of Withania somnifera (l.) Dunal. Int J Pharma Bio Sci 2010;1:1-5.

Sharada M, Ahuja A, Suri KA, Vij SP, Khajuria RK, Verma V, et al. Withanolide production by in vitro cultures of Withania somnifera (L.) and its association with differentiation. Biol Plant 2007;51:161-4.

Sivanandhan G, Arun M, Mayavan S, Rajesh M, Mariashibu TS, Manickavasagam M, et al. Chitosan enhances withanolides production in adventitious root cultures of Withania somnifera (L.) Dunal Ind Crops Prod 2012;37:124-9.

Abraham A, Kirson I, Glotter E, Lavie DA. Chemotaxonomic study of Withania somnifera (L.) dunal. Phytochemistry 1968;7:957–62.

Singariya P, Kumar P, Mourya KK. Identification of new bioactive compounds by GC-MS and estimation of physiological and biological activity of kala dhaman (Cenchrus setigerus). Int J Pharm Biol Arch 2012;3:610-6.

Stashenko EE, Martínez JR. GC-MS Analysis of Volatile Plant Secondary Metabolites. In: Gas Chromatography in Plant Science. Salih B. (Eds.); 2012. p. 402-20.

Murthy HN, Dijkstra C, Anthony P, White DA, Davey MR, Power JB, et al. Establishment of Withania somnifera hairy root cultures for the production of withanolide A. J Integr Plant Biol 2008;50:975–81.

Styczynski MP, Moxley JF, Tong LV, Walther JL, Jensen KL. Systematic identification of conserved metabolites in GC/MS data for metabolomics and biomarker discovery. Anal Chem 2007;79:966-73.

Erisson L, Johansson E, Kettaneh-Wold N, Trygg J, Wikstrom C. Multi-and megavariate data analysis. In: Kingston SJ. (Ed.). Basic principles and applications, Umetrics Academy Umea, Umea, Sweden; 2006. p. 195-9.

Adams P, Thomas JC, Vernon DM, Bohnert HJ, Jensen RG. Distinct cellular and organismic responses to salt stress. Plant and Cell Physiology 1992;33:1215-23.

Vernon DM, Bohnert HJ. A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum crystallinum. EMBO J 1992;11:2077-85.

Loewus FA, Kelly S. Conversion of glucose to inositol in parsley leaves. Biochem Biophys Res commun 1962;7:204-8.

Funkhouser EA, Loewus FA. Purification of myoinositol 1-phosphate synthase from rice cell culture by affinity chromatography. Plant Physiol 1975;56:786-90.

Loewus MW, Loewus FA. D-Glucose-6-phosphate cycloaldolase: Inhibition studies and aldolase function. Plant Physiol 1973;51:263-6.

Nelson DE, Koukoumanos M Bohnert HJ. Myo-Inositol-dependent sodium uptake in ice plant. Plant Physiol 1999;119:165–72.

Läuchli A, Grattan S. Plant growth and development under salinity stress. In: Advances in molecular breeding toward drought and salt tolerant crops. Jenks MA, Hasegawa PM, Jain SM. (Eds.). Dordrecht, Springer; 2007. p. 1–32.

Bouche F, Fromm H. GABA in plants: just a metabolite? Trends Plant Sci 2004;9:110-5.

Roberts MR. Does GABA act as a signal in plants? Plant Signaling Behav 2007;2:408-9.

Li XG, Zhang TL, Wang XX, Hua K, Zhao L, Han ZM. The composition of root exudates from two different resistant peanut cultivars and their effects on the growth of soil-borne pathogen. Int J Biol Sci 2013;9:164–73.

Tiburcio AF, Campos JL, Figueras X, Besford RT. Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regul 1993;12:331-40.

Galston AW, Kaur-Sawhney R, Altabella T, Tiburcio AF. Plant polyamines in reproductive activity and response to abiotic stress. Bot Acta 1997;110:197-207.

Bais HP, Ravishankar GA. Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Cult 2002;69:1-34.

Desbrosses GG, Kopka J, Udvardi MK. Lotus japonicus metabolic profiling: development of gas chromatography-mass spectrometry resources for the study of plant-microbe interactions. Plant Physiol 2005;137:1302–18.

Dey P, Chaudhuri TK. Antioxidant capacity of N. indicum:a correlation study using principal component analysis and multivariate statistical approach. Int J Pharm Pharm Sci 2013;5:931-7.



How to Cite

Thirugnanasambantham, P., K. Senthil, T.- joo Oh, and H. K. Choi. “COMPARATIVE CHEMOMETRIC PROFILES BETWEEN LEAF TISSUES OF WITHANIA SOMNIFERA CULTURED IN VITRO AND FIELD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 66-71,



Original Article(s)