• Priyanka Dash Department of Pharmacognosy, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Khandagiri Square, Bhubaneswar 751003, Odisha, India
  • Goutam Ghosh Siksha ‘O’ Anusandhan University


Citrullus lanatus, Milk clotting, Casein


Objective: The objective of the present investigation was to evaluate milk clotting activity as well as proteolytic activity of protein fractions of seeds of Citrullus lanatus.

Methods: Storage proteins were extracted by differential solubility, and their contents were estimated using the Bradford method. The effect of pH and temperature on the milk-clotting activity and proteolytic activity was also evaluated.

Results: Citrullus lanatus showed highest milk clotting activity over a broad temperature range of 30-80 ºC and pH range of 3-9.

Conclusion: These findings showed that Citrullus lanatus have proteolytic activity which might be a potentially suitable substitute for commercial animal rennet for cheese-ripening.



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How to Cite

Dash, P., and G. Ghosh. “PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 380-3,



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