PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS

Authors

  • Priyanka Dash Department of Pharmacognosy, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan University, Khandagiri Square, Bhubaneswar 751003, Odisha, India
  • Goutam Ghosh Siksha ‘O’ Anusandhan University

Keywords:

Citrullus lanatus, Milk clotting, Casein

Abstract

Objective: The objective of the present investigation was to evaluate milk clotting activity as well as proteolytic activity of protein fractions of seeds of Citrullus lanatus.

Methods: Storage proteins were extracted by differential solubility, and their contents were estimated using the Bradford method. The effect of pH and temperature on the milk-clotting activity and proteolytic activity was also evaluated.

Results: Citrullus lanatus showed highest milk clotting activity over a broad temperature range of 30-80 ºC and pH range of 3-9.

Conclusion: These findings showed that Citrullus lanatus have proteolytic activity which might be a potentially suitable substitute for commercial animal rennet for cheese-ripening.

 

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References

Estelle M. Proteases and cellular regulation in plants. Curr Opin Plant Biol 2001;4:254–60.

Pontual EV, Carvalho BEA, Bezerra RS, Coelho LCBB, Napoleão TH, Paiva PMG. Caseinolytic and milk-clotting activities from Moringa oleifera flowers. Food Chem 2012;135:1848–54.

Ahmed IAM, Babiker EE, Mori N. pH stability and influence of salts on activity of a milk-clotting enzyme from Solanum dubium seeds and its enzymatic action on bovine caseins. LWT-Food Sci Technol 2010;43:759–64.

Egitoa AS, Girardetc JM, Lagunaa LE, Poirsonc C, Molle´bD, Micloc L, et al. Milk-clotting activity of enzyme extracts from sunflower and albizia seeds and specific hydrolysis of bovine k-casein. Int Dairy J 2007;17:816–25.

Bruno MA, Lazza CM, Errasti ME, Lo´ pez LMI, Caffini NO, Pardo MF. Milk clotting and proteolytic activity of an enzyme preparation from Bromelia hieronymi fruits. LWT-Food Sci Technol 2010;43:695–701.

Tavaria FK, Souza MJ, Domingos A, Malcata FX, Brodelius P, Clemente A, et al. Degradation of caseins from milk of different species by extracts of Centaurea calcitrapa. J Agric Food Chem 1997;45:3760–5.

Ahmed IAM, Morishima I, Babiker EE, Mori N. Characterisation of partially purified milk-clotting enzyme from Solanum dubium Fresen seeds. Food Chem 2009;116:395–400.

Yadav S, Tomar AK, Jithesh O, Khan MA, Yadav RN, Srinivasan A, et al. Purification and partial characterization of low molecular weight Vicilin-Like glycoprotein from the Seeds of Citrullus lanatus. Protein J 2011;30:575–80.

Teugwa CM, Boudjeko T, Tchinda BT, Mejiato PC, Zofou D. Anti-hyperglycaemic globulins from selected Cucurbitaceae seeds used as antidiabetic medicinal plants in Africa. BMC Complementary Altern Med 2013;13:63.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-54.

Shieh C, Thi LP, Shih I. Milk-clotting enzymes produced by culture of Bacillus subtilis natto. Biochem Eng J 2009;43:85–91.

Corrons MA, Bertucci JI, Liggieri CS, López LMI, Bruno MA. Milk clotting activity and production of bioactive peptides from whey using Maclura pomifera proteases. LWT-Food Sci Technol 2012;47:103-9.

Mazorra-Manzano MA, Perea-Gutiérrez TC, Lugo-Sánchez ME, Ramirez-Suarez JC, Torres-Llanez MJ, González-Córdova AF, et al. Comparison of the milk-clotting properties of three plant extracts. Food Chem 2013;141:1902–7.

Monica G, Parisi MG, Moreno S, Fernandez G. Isolation and characterization of a dual function protein from Allium sativum bulbs which exhibits proteolytic and hemagglutinating activities. Plant Physiol Biochem 2008;46:403-13.

Kaneda M, Tominaga N. Isolation and characterization of a proteinase from the sarcocarp of melon fruit. J Biochem 1975;78:1287–96.

D’Ambrosio A, Rossano R, Ungaro N, Riccio P. Proteolytic and milk clotting activities in extracts obtained from the crustaceans Munida. J Mol Catal B: Enzym 2003;22:145–50.

Asif-Ullah M, Kim KS, Yu YG. Purification and characterization of a serine protease from Cucumis trigonus Roxburghi. Phytochemistry 2006;67:870–5.

Yadav SC, Jagannadhan MMV. Highly stable glycosylated serine protease from medicinal plant Euphorbia milii. Phytochemistry 2006;67:1414–26.

Vieille C, Zeikus JG. Thermozymes: Identifying molecular determinant of protein structural and functional stability. Trends in Biotechnol 1996;14:183–9.

Silvestre M, Carreira R, Silva M, Corgosinho F, Monteiro M, Morais H. Effect of pH and temperature on the activity of enzymatic extracts from pineapple peel. Food Bioprocess Technol 2012;5:1824–31.

Published

01-11-2015

How to Cite

Dash, P., and G. Ghosh. “PROTEOLYTIC AND MILK CLOTTING ACTIVITY OF FRACTIONATED PROTEIN OF CITRULLUS LANATUS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 11, Nov. 2015, pp. 380-3, https://journals.innovareacademics.in/index.php/ijpps/article/view/8248.

Issue

Section

Short Communication(s)