• Sri Wahyuningsih School of Pharmacy, Bandung Institute of Tecnology, Jalan Ganeca no. 10 Bandung,Indonesia
  • Elin Yulinah Sukandar
  • Sukrasno .
  • Debby Nadya Lofika


Objective: The objective of this study was to determine anti-hyperuricemia activity of ethanol extract of roselle calyx (Hibiscus sabdariffa L) and its fraction on male Wistar rats.

Methods: Two different methods were used. First method induction was done by giving potassium oxonate and diet high purine whereas allopurinol as a comparison. The parameter was level uric acid in serum (uricostatic activity). Second methods induction was done by giving potassium oxonate intraperitoneally and sodium urate orally; with pprobenecid as comparison Parameters used were uric acid level in serum and urine (uricosuric activity).

Results: Ethanol extract roselle calyx at a dose of 40 mg/kg bw, water fraction at a dose of 37.5 mg/kg bw and ethyl acetate fraction at a dose of 6.25 mg/kg bw at day 14 showed a significant reduction in serum uric acid level compared to control group (p<0.05). Besides that ethanol extract of Roselle calyx at dose 80 mg/kg bw, water fraction at doses 37.5 and 75 mg/kg bw also ethyl acetate at doses 6.25 and 12.5 mg/kg bw had uricosuric effect that increased the excretion of uric acid in urine significantly compared to control group (p<0.05).

Conclusion: The water fraction of roselle calyx at doses 37.5 and 75 mg/kg bw and ethyl acetate at dose 6.25 and 12.5 mg/kg bw was effective as an anti-hyperuricemic agent. The ethanol extract of rosele calyx and its fraction had a uricosuric effect similar to probenecid. The best uricosuric effect was showed by ethyl acetate fraction at dose 6.25 mg/kg bw.

Keywords: Hibiscus sabdariffa Linn, Water fraction, Ethyl acetate fraction, Calyx, Uric acid, Antihyperuricemia


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Darmawan J, Valkenburg HA, Muirden KD, Wigley RD. The epidemiology of gout and hyperuricemia in a rural population of Java. J Rhematol. The Netherlands: Erasmus University; 1992.

Kambayana G, Putra TR. Hyperuricemia and related in balinese population. Int J Rheum Dis 2010;13:211-20.

Wallace KL, Riedel A, Ridge NJ, Wortmann R. Increasing prevalence of gout and hyperuricemia over 10 y among older adults in a managed care population. J Rheumatol 2004;31:8.

Dipiro JT, Talbert RL, Yee GC, Matzke G, Weel B, Possey LM. Pharmacotherapy: a pathophysiologic approach. 7 th ed. NewYork, Mc Graw Hill; 2008.

Zhao X, Zhu X, Pan Y. Effects of cassia oil on serum and hepatic uric acid levels in oxonate-induced mice and xanthin dehydrogenase and xanthine oxidase activities in mouse liver. J Ethnopharmacol 2006;103:357-65.

Widiyanto PS, Nelistya A. Rosela aneka olahan, Khasiat dan Ramuan, Penebar Swadaya; 2009. p. 11-4, 21.

Vogel GH, Drug discovery and evaluation, pharmacological essays. 2nd edition. Springer-Verlag-Berlin-New York; 2002. p. 339-49.

Yonetani Y, Iwaki K. Effects of uricosuric drug and diuretics on uric acid excretion in oxonate-treated rats. Jpn J Pharmacol 1983;33:947-57.

Afrianti LH, Sukandar EY, Adriyana IK, Ibrahim S. Antihiperuricemic activity of ethyl acetate and ethanol extract of snake fruit var bongkok (Salaca edulis Reinw) on wistar rat. J Teknol Dan Industry Pangan 2011;22:7-10.

Oxada Y, Tsuchimoto M, Fukushuma H, Takashi K, Kondo S, Hasegawa M. Hyperuricemic of the novel xanthine oxidase inhibitor, TEI-6720, in Rodent. Eur J Pharmacol 1993,241:183-8.

Ni Luh Putu NADW, Efek Asupan Emping Goreng (produk olahan melinjo) terhadap Kadar asam urat darah Laki laki dewasa; 2009.

Sugino H, Shimada H. The uricosuric effect in rats of E5050 a new derivate of ethanolamine, involves inhibition of the tubular post-secretory reabsorption of urate. Jpn J Pharmacol 1995;68:297-303.

Yu Z, Fong WP, Cheng CHK. The dual actions of morin (3,5,7,2, 4–Pentahydroxyflavone) as a hypouricemic agent: uricosuric effect and xanthine oxidase inhibitory activity. J Pharmacol Exp Ther 2005;316:169-75.

Huang CG, ShangYJ, Zhang J, Zang JR. Hypouricemic effects of phenylpropanoid glycosides acetonide of Scrophularia ningpoensis on serum uric acid levels in potassium oxonate–pretreated mice. Am J Chin Med 2008;36:149-57.

Murugaiyah V, Chans KL. Mechanisms of the anti hyperuricemic effect of Phylantus niruri and its ligands constituents. Ethnopharm 2009;124:233-9.



How to Cite

Wahyuningsih, S., E. Y. Sukandar, S. ., and D. N. Lofika. “ANTIHYPERURICEMIA ACTIVITY OF THE ETHANOL EXTRACT OF ROSELLE CALYX AND ITS FRACTION (HIBISCUS SABDARIFFA LINN) ON MALE WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 3, Mar. 2016, pp. 278-80,



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