ANTIOXIDANT ACTIVITIES FROM VARIOUS LEAVES EXTRACTS OF THREE CULTIVARS OF PAPAYA FROM WEST JAVA-INDONESIA
Abstract
ABSTRACT
Objectives: The goals of this research were to determine antioxidant activity from various leaves extracts of three cultivars of papaya using two
methods of antioxidant testing which were 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS),
and correlation of total phenolic content (TPC), total flavonoid content (TFC), and total carotenoid content (TCC) in various extracts of papaya leaves
with their IC
50
of DPPH and IC
50
of ABTS.
Methods: Extraction was carried out by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator.
Antioxidant activities using DPPH and ABTS assays, determination of TPC, TFC, and TCC were performed by ultraviolet-visible spectrophotometry
and correlation with their IC
Results: The lowest IC
50
50
of DPPH and IC
50
of ABTS scavenging activities were analyzed by Pearson's method.
of DPPH scavenging activity 0.84 µg/ml was given by ethanolic leaves extract of calina papaya, whereas ethanolic leaves
extract of Bangkok papaya showed the lowest IC
of ABTS scavenging activity 1.79 µg/ml. Ethanolic leaves extract of burung papaya had the highest
phenolic content and its ethyl acetate extract had the highest total flavonoid. There were significantly negative correlation between TPC in Bangkok
papaya and calina papaya extract with their IC
50
50
of DPPH.
Conclusions: All of the leaves extracts from three cultivars of papaya were categorized as very strong antioxidant by DPPH and ABTS methods.
Phenolic compounds in Bangkok papaya and calina papaya were the major contributor in IC
of DPPH scavenging activities. DPPH and ABTS gave no
linear result in antioxidant activities of leaves extract of three cultivars of papaya.
50
Keywords: 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Leaves, Papaya, Three cultivars.
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