PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s2.13526Abstract
ABSTRACT
Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl
(DPPH)
and
phosphomolybdenum
assays,
total
phenolic,
flavonoid,
and
carotenoid
content,
correlation
of
total
phenolic,
flavonoid,
and
carotenoid
content
in
various
extracts
of
chayote
with their
inhibitory
concentration
50%
(IC
) of DPPH antioxidant activities and exhibitory
concentration 50% (EC
50
) of phosphomolybdenum capacity, and correlation between two antioxidant assays.
50
Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant
activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible
spectrophotometry
and its correlation
with IC
Results: The lowest IC
50
50
of DPPH and EC
50
of phosphomolybdenum was analyzed by Pearson's method.
of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 µg/ml), whereas the lowest EC
of
phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 µg/ml). Ethyl acetate cortex extract of pomelo had the
highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant
correlation between TFC in cortex and peel extracts of pomelo with their IC
50
of DPPH.
Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid
compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum
assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo.
Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs.
50
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References
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