THES STUDY OF ETHANOLIC EXTRACT OF BINAHONG LEAVES (ANREDERA CORDIFOLIA (TEN.) STEENIS) AND MULBERRY LEAVES (MORUS NIGRA L.) IN COMBINATION ON HYPERLIPIDEMIC INDUCED RATS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.14412Abstract
Objective: This study aimed to determine antihyperlipidemic activity of binahong leaves and murbey leaves extracts in combination.
Methods: A total of 28 rats were divided into seven groups: normal, control, simvastatin 3.6 mg/kg bw, binahong leaves extract 100 mg/kg bw (B100), mulberry leaves extract 200 mg/kg bw (M200), combination of binahong extract 50 mg/kg and mulberry 100 mg/kg (B50+M100), and combination of binahong extract 100 mg/kg bw and mulberry 200 mg/kg bw (B100+M200). Hyperlipidemia was induced by giving rats a high fat diet along with cholesterol (200 mg/kg), cholic acid (0.2% of chow), and propylthiouracil (10 mg/kg bw) for 30 days orally. Then, for the next 14 days, all groups except the control were given the tested substances. Measurement of lipid profile was performed immediately after induction (T0), seven and fourteen days after treatment (T7 and T14, respectively).
Results: There were significant differences on a group of M200, B50+M100, and B100+M200 compared to the control group at T14, with the value 33.68%, 34.39 %, and 44.81% (in reduction of total cholesterol) and 36.86%, 37.16%, and 49.99% (in reduction of triglyceride).
Conclusion: Group of M200, B50+M100, and B100+M200 reduced total cholesterol and triglyceride level remarkably at day 14. The combination of both extract (B100+M200) considerably shows better activity than either binahong extract or mulberry extract.Â
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References
Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM. 2011, Pharmacotherapy A Patophysiologic Approach, ed 8, Columbia: McGraw Hill; 2011.p. 1267 –79.
National Cholesterol Education Program (NCEP). ATP III Guidelines At-A-Glance Quick Desk Reference, United Stateds: National Institute of Health; 2011. 01-3305.
American Heart Association (AHA) Statistics Committee and Stroke Statistics Subcommittee, 2013, Heart disease and stroke statistics—2013 update: a report from the American Heart Association, American Heart Association, United States, Circulation. 2013;127:e6-e245.https://www.heart.org/idc/groups/heart-public/@wcm/@sop/@smd/documents/ downloadable/ucm_319586.pdf
Lestari D, Sukandar EY, Fidrianny I. Anredera cordifolia Leaves Extract as Antihyperlipidemia and Endothelial Fat Content Reducer in Male Wistar Rat. Int J Pharm Clin Res 2015;7(6): 435-9
Hou Y, Shao W, Xiao R, Xu K, Ma Z, Johnstone BH, Du Y. Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat hyperlipidemia model. Exp Gerontol 2009; 44(6): 434-9.
Lestari D. Efek Kombinasi Ekstrak Etanol Rimpang Rumput Teki (Cyperus rotunda L.) dan Daun Mulberry (Morus alba L.) terhadap Kolesterol dan Hambatan Pembentukan Plak Ateroma Pada Tikus Wistar Jantan. Theses Book of Master Degree, Bandung: Institut Teknologi Bandung; 2012
Rahman AA, 2015, Efek Preventif Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Peningkatan Kadar Kolesterol Pada Tikus Wistar Jantan Yang Diberi Makanan Tinggi Kolesterol. Theses of Master Degree, Bandung: Institut Teknologi Bandung; 2015
World Health Organization (WHO), 2012, Cardivascular Diseases Fact Sheets, World Health Organization (WHO), http://www.who.int/mediacentre/factsheets/fs317/en/
Woods SC, Seeley RJ, Rushing PA, D’Alessio D, Tso P. A controlled high-fat diet induces an obese syndrome in rats. J Nutr 2003; 133(4): 1081-7.
Balakumar P, Babbar L. Preconditioning the hyperlipidemic myocardium: Fact or fantasy? Cell Signal 2012;24(3):589-95.
Yadav HN, Singh M, Sharma PL. Modulation of the cardioprotective effect of ischemic preconditioning in hyperlipidaemic rat heart. Eur J Pharmacol 2010;643(1):78-83.
Munshi RP, Joshi SG, Rane BN. Development of an experimental diet model in rats to study hyperlipidemia and insulin resistance, markers for coronary heart disease. Indian J Pharmacol 2014; 46(3): 270.
Sukandar EY, Sigit JI, Adiwibowo LF. Study of Kidney Repair Mechanisms of Corn Silk (Zea mays L. Hair)-Binahong. Int J Pharmacol 2013; 9(1):12-23.
Widyarini KD, Sukandar EY, Fidrianny I. Xanthine oxidase inhibitory and antihyperuricemic activities of Anredera cordifolia (Ten) steenis, Sonchus arvensis L, and its combination. Int J Pharm Pharm Sci 2015; 7(3):86-90.
Garmana AN, Sukandar EY, Fidrianny I. Activity of several plant extracts against drug-sensitive and drug-resistant microbes. Procedia Chemistry 2014; 13:164-9.
Indriyanti N, Adnyana IK, Sukandar EY. Studi aktivitas antifungi dari binahong Anredera cordifolia dan akar Petiveria alliacea. The Final Project Book of Undergraduate Students, Bandung: Institut Teknologi Bandung; 2011.
Garmana AN, Sukandar EY, Fidrianny I. Preliminary Study of Blood Pressure Lowering Effect of Anredera cordifolia (Ten.) Steenis) on Wistar Rats. International Journal of Pharmacognosy and Phytochemical Research 2016; 8(2); 300-4
Salasanti CD, Sukandar EY, Fidrianny I. Acute and sub chronic toxicity study of ethanol extract of Anredera cordifolia (Ten.) v. Steenis leaves. Int J Pharm Pharm Sci 2014; 6(5): 348-52.
Urmila GH, Satyanarayana T, Ganga Rao B. Antihyperlipidemic activity of methanolic leaf extract of three different plants on high cholesterol diet induced hyperlipidemic rats, Int J Preclinic Pharm Res 2014; 5 (1) : 30-3.
Juwita R, Sukandar EY, Fidrianny I, Patonah. Uji efek antihiperlipidemia ekstrak etanol daun binahong (Anredera cordifolia) terhadap tikus Wistar jantan dengan metode homeostatis kolesterol. The Final Project Book of Undergraduate Students, Bandung: Institut Teknologi Bandung; 2011.
Sukandar EY, Kurniati NF, Nurdianti AN. Antiobesity effect of ethanol extract of anredera cordifolia (ten.) Steenis leaves on obese male wistar rats induced by high_carbohydrate diet. Int J Pharm Pharm Sci 2016; 8(4):171-3.
Souza LF, de barros IB, Mancini E, de martino L,Scandolera E, de Feo V. Chemical composition and biological activities of the essential oil from Anredera cordifolia grown in Brazil. Nat Prod Commun 2014; 9: 1003-6.
Sukandar EY, Kurniati NF. Evaluation of teratogenicity effects of ethanolic extracts of binahong leaves (Anredera Cordifolia (Ten) steenis) in wistar rat. Int J Pharm Pharm Sci 2014; 6(11):422-6.
Sukandar EY, Fidrianny I, Nofianti T, Safitri D. 2016. Subchronic Toxicity Study of Corn Silk (zea mays l.) in Combination with Binahong (Anredera cordifolia (ten.) Steenis leaves on Wistar Rats. Asian J Pharm Clin Res 2016; 9(1), 292-6.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4):555-559.
Chen CC, Liu LK, Hsu JD, Huang HP, Yang MY, Wang CJ. Mulberry extract inhibits the development of atherosclerosis in cholesterol-fed rabbits. Food Chem 2005; 91(4):601-7.
Gerasopoulos D, Stavorulakis G. Quality characteristics of four mulberry (Morus sp.) cultivars in the area of China, Greece. J Sci Food Agric 1997; 73: 261–4.
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