IDENTIFICATION AND CHARACTERIZATION OF POTENT ANTIMICROBIAL SECONDARY METABOLITE GENERATED FROM ENDOPHYTIC FUNGI ISOLATED FROM                 JAMBLANG PLANT ( Eugenia cumini L)

SHIRLY  KUMALAl; NG VINI  APRILIA; PARTOMUAN  SIMANJUNTAK

doi:10.22159/ajpcr.2017.v10i4.16725

Authors

SHIRLY KUMALAl Faculty of Pharmacy Pancasila University Jakarta Indonesia
NG VINI APRILIA
PARTOMUAN SIMANJUNTAK

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.16725

Abstract

Objective: Colletotrichium capsiciÂ endophytic fungi isolated from Jamblang plant (Eugenia cumini Â L). Â The secondary metabolites of this plant has the potent antibacterial efficacy Â as well asÂ diarrheal and anti-diabetic. This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites.

Methods: Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates with strongest antimicrobial activity against the bacteriaÂ Staphylococcus aureus, Escherichia coli wereÂ fermented inÂ Potato Dextrose YeastÂ (PDY) to produce Â large scale of the metabolites. Â Supernatant was extracted with ethyl acetate solvent. Ethyl acetate extract fractionated by column chromatography (SiO3,Â n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity.

Results: Â Antibacterial Â test Â results Â indicatedÂ that fraction IIIÂ had Â the antibacterial Â activityÂ StaphylococcusÂ aureusÂ with Â inhibition zoneÂ diameter Â of 10.7Â mm but no observed Â antibacterial activityÂ againstÂ EscherichiaÂ coli. Â Furthermore, identificationÂ byÂ GC-MS showed that compounds present in fraction III was mainly fatty acid and phenolic compounds.

Conclusion: Â In conclusion, secondary metabolites isolated from Jamblang plantsÂ branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti microbial activity.

Â Keywords : Endophytic fungi, Jamblang (Eugenia cumini L.), antimicrobial activity

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